Mestrado em Ciências Veterinárias
URI Permanente para esta coleção
Nível: Mestrado Acadêmico
Ano de início: 2008
Conceito atual na CAPES: 4
Ato normativo: Homologado pelo CNE (PORTARIA 609 de 14 de março de 2019) Publicado no D.O.U - 18/03/2019
Periodicidade de seleção: Semestral
Área(s) de concentração: Ciências Veterinárias
Url do curso: https://cienciasveterinarias.ufes.br/pt-br/pos-graduacao/PPGCV/detalhes-do-curso?id=1451
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Submissões Recentes
- ItemPerfil produtivo e endoparasitário da bovinocultura leiteira do município de Alegre–ES: uma análise multivariada(Universidade Federal do Espírito Santo, 2025-07-09) Almeida, Rafael Assis Torres de; Torres, Rodrigo de Nazaré Santos; https://orcid.org/0000-0002-0453-542X; http://lattes.cnpq.br/8459074474066495; Almeida, Marco Túlio Costa; https://orcid.org/0000-0001-8730-0425; http://lattes.cnpq.br/7297285577963405; http://lattes.cnpq.br/0949110783844972; Martins, Isabella Vilhena Freire; https://orcid.org/0000-0002-8700-3065; http://lattes.cnpq.br/6446528818006897; Guerson, Yuri Barbosa; https://orcid.org/0000-0001-7796-0921; http://lattes.cnpq.br/6290312600632479 ; Rego, Rafael Otaviano do; https://orcid.org/0000-0001-9291-7219; http://lattes.cnpq.br/7032024290799824Despite ongoing evolution in milk production, it's crucial to acknowledge potential obstacles that could adversely impact profitability if not properly identified and addressed. This study aimed to assess production profiles and endoparasite prevalence in dairy herds in Alegre, ES, using multivariate analysis. Research was conducted on local farms using surveys and fecal samples from cattle. Descriptive statistics and principal component analysis (PCA) were used to identify the main factors influencing dairy farming in the region.Findings revealed that 79% of farms employed family labour, 46% received technical support, and 82% of producers resided on site. Farms averaged 44 hectares with approximately 50 predominantly Girolando breed cattle. Average daily production was 96 litres per farm and 7.1 litres per animal. Seventy percent practised rotational grazing, with milk chilled in communal (46%) or individual (50%) tanks. Health management was robust, with vaccination rates exceeding 93% for rabies, clostridiosis, and brucellosis. Bovine parasitic diseases, notably helminthiases (27%), were prevalent. Cypermethrin (42%) and levamisole (73%) were commonly used for parasite control. Natural mating predominated for reproductive management (33%), often combined with biotechnologies (50%). Endoparasite prevalence analysis from 97 adult mixed breed cattle showed helminthiases at 64%, varying by severity. Eggs of Strongylidea (77%), Trichuris sp. (6%), Moniezia sp. (13%), and Ascaridae (13%) were identified. Coccidiosis affected 74% of farms, particularly in young animals (68%), often co-occurring with helminth eggs (77.4%). Fasciola hepatica was found in 16.1% of farms. Multivariate analysis highlighted variables such as animal count, daily farm milk production, paddock numbers, milk yield per hectare, labour, farm size, EPG (eggs per gram), OOPG (oocysts per gram), and rainfall patterns. These findings underscore the importance of targeted policies and programs to bolster Alegre's dairy sector and enhance parasite management. Integrating traditional and modern practices, along with robust technical support and effective sanitary practices, is pivotal for
- ItemSoroprevalência e fatores de risco do coronavírus felino (FCoV) no sul capixaba(Universidade Federal do Espírito Santo, 2025-03-25) Caprioli, Ana Amélia; Delatorre, Edson Oliveira; https://orcid.org/0000-0002-5746-0820; http://lattes.cnpq.br/9814839314541002; https://orcid.org/0009-0000-1875-5303; http://lattes.cnpq.br/2835032810980724; Guerson, Yuri Barbosa; https://orcid.org/0000-0001-7796-0921; http://lattes.cnpq.br/6290312600632479; Archanjo, Anderson Barros; https://orcid.org/0000-0002-9305-271X; http://lattes.cnpq.br/5529149503714764Coronaviruses (CoVs) are zoonotic viruses belonging to the Coronaviridae family, capable of infecting various species, including felines. Feline Coronavirus (FCoV) can evolve into feline infectious peritonitis (FIP), a severe and often fatal disease. This study aimed to investigate the seroprevalence of FCoV in domestic cats from the municipalities of Alegre and Jerônimo Monteiro, in southern Espírito Santo, and to identify the main risk factors associated with infection. Blood samples were collected from 120 cats, aged between 41 days and 13 years, treated at the Patinhas Alegres veterinary clinic. Antibody detection was performed using the ImmunoComb FCoV test, an ELISA-based immunoassay that quantifies serological levels into categories of negative, weak, moderate, and strong positive. The seropositivity rate was 69.2%, with a distribution of 5.8% weak positive, 29.2% moderate positive, and 34.2% strong positive. Univariate analysis indicated a significant association between FCoV infection and age (p = 6.18 × 10⁻⁷) and reproductive status (p = 3.09 × 10⁻⁸), while sex (p = 0.4035) and housing conditions (p = 0.2628) were not statistically significant. Binary logistic regression confirmed that cats aged 3–8 years had an 11-fold higher risk of infection (OR = 10.96; 95% CI: 2.56 – 56.57; p = 0.002), and cats older than 8 years had a 9-fold increased risk (OR = 9.27; 95% CI: 1.42 – 86.91; p = 0.029), suggesting that cumulative exposure to the virus over time is a key determinant of infection. Ordinal logistic regression revealed that older cats not only had higher seropositivity rates but also exhibited elevated antibody levels. Compared to kittens (<1 year), cats aged 3–8 years were 8.8 times more likely to have high antibody titers (OR = 8.84; 95% CI: 2.45 – 34.94; p = 0.0012), while cats older than 8 years had a 19-fold increased risk (OR = 19.12; 95% CI: 4.11 – 100.37; p = 0.0003). Housing conditions showed a statistical trend, with cats living alone exhibiting a higher infection risk (OR = 9.48; 95% CI: 1.08 – 216.87; p = 0.041). However, this factor did not significantly influence antibody levels, suggesting that social contact may affect viral transmission but not necessarily the immune response. Given these findings, controlling FCoV infection in Brazil requires not only large-scale and continuous epidemiological studies to monitor high-risk groups, such as adult and elderly cats, but also the implementation of integrated biosecurity measures, including proper environmental management, targeted vaccination protocols, and increased awareness among cat owners to prevent viral spread and minimize the risk of progression to FIP.
- ItemO impacto da Haematobia irritans na prevalência da mastite subclínica em rebanhos leiteiros(Universidade Federal do Espírito Santo, 2025-03-12) Serafim Júnior, Alclecir Bitencourt; Torres, Rodrigo de Nazaré Santos; https://orcid.org/0000-0002-0453-542X; http://lattes.cnpq.br/8459074474066495; Almeida, Marco Túlio Costa; https://orcid.org/0000-0001-8730-0425; http://lattes.cnpq.br/7297285577963405; https://orcid.org/0009-0006-4125-2572; http://lattes.cnpq.br/6571871986330673; Valença, Roberta de Lima; https://orcid.org/0000-0003-1341-1602; http://lattes.cnpq.br/8772231390273217; Silva, Maria Aparecida da; https://orcid.org/0000-0003-0967-3925; http://lattes.cnpq.br/9360093834818219The present study aimed to evaluate the ability of the horn fly (Haematobia irritans) to carry bacterial agents causing subclinical mastitis in cattle, relating the microbiota present on the surface of these insects to the identification of bacteria in milk samples and swabs from the teat surfaces of cows diagnosed with subclinical mastitis. The study was conducted on two dairy farms in the municipality of Alegre, Espírito Santo, Brazil, where technical visits were carried out to assess sanitary conditions and milk production management.The identification of cows with subclinical mastitis was performed using the California Mastitis Test (CMT), and milk and teat surface swab samples were collected from positive animals. Horn flies were captured in the milking area, near the animals. The samples were subjected to bacteriological cultures on MacConkey Agar, Brain Heart Infusion (BHI) Agar, and Sabouraud Agar, and the isolated colonies were characterized based on colony morphology, Gram staining, and biochemical tests for catalase and coagulase. The results indicated that Staphylococcus aureus was the main etiological agent isolated, being present in most milk and teat swab samples from cows with subclinical mastitis. Additionally, microbiological analysis of the flies revealed the presence of S. aureus, Gram-positive bacilli, and Gram-negative bacilli, demonstrating that these insects can act as mechanical vectors for pathogen transmission in the milking environment. The multivariate statistical analysis (PCA) revealed a strong association between the fly microbiota and the presence of S. aureus in milk and teat samples, especially in Farm 1, where the pathogen was predominant. In Farm 2, Streptococcus spp. was identified more frequently, suggesting a closer relationship between milking hygiene and the presence of these microorganisms. Based on these findings, it is concluded that the horn fly acts as a vector for bacterial agents associated with subclinical mastitis in cattle, making it an epidemiologically relevant factor in the dairy production chain. Thus, effective control of this ectoparasite is essential to reduce the spread of microorganisms in the milking environment and mitigate the impact of subclinical mastitis on dairy productivity. Furthermore, continuous monitoring of the isolated bacteria can support more effective sanitary management strategies on dairy farms, minimizing economic losses and improving milk quality.
- ItemAtividade antifúngica e antibiofilme de derivados triazólicos de eugenol e timol frente a Candida spp(Universidade Federal do Espírito Santo, 2025-04-28) Gouvêa, Monique Vargas de; Ignacchiti, Mariana Drummond Costa; https://orcid.org/0000-0003-3768-949X; http://lattes.cnpq.br/0533640513430050; Resende, Juliana Alves; https://orcid.org/0000-0002-5476-3754; http://lattes.cnpq.br/8223821041049149; https://orcid.org/0009-0000-6897-0167; http://lattes.cnpq.br/7150204118206054; Costa, Adilson Vidal; https://orcid.org/0000-0002-7968-8586; http://lattes.cnpq.br/1638000107452454; Dias, Vanessa Cordeiro; https://orcid.org/0000-0001-7681-3268; http://lattes.cnpq.br/5371121263113723Fungal infections, mainly caused by species of the genus Candida, represent a growing global health problem. Available treatment is limited, with emphasis on azoles, widely used due to their broad spectrum, efficacy, and safety. However, the development of antifungal resistance has compromised their efficacy, highlighting the need for new molecules. This study evaluated the antifungal activity of 09 triazole compounds derived from eugenol and 19 derivatives of thymol against Candida albicans and non-albicans Candida spp. The results demonstrated that the eugenol-derived compounds 3a, 3e and 3i, and the thymol derivative 3g, have significant antifungal action, with antibiofilm activity, acting on the permeability, cell wall and ergosterols of the cell membrane of several Candida species. 3e presented more homogeneous MIC and MFC values among the species tested, ranging from 125 to 250 ppm, and presented relevant values in biofilm inhibition, especially against Pichia kudriavzevii (Candida krusei) and Nakaseomyces glabratus (Candida glabrata). In the disarticulation of mature biofilms, it demonstrated significant results for C. albicans. It also promoted substantial changes in the integrity of the cell membrane, particularly in P. kudriavzevii (C. krusei) and Candida tropicalis. The triazole derivative 3g demonstrated superior antifungal and antibiofilm activity to pure thymol against Candida species, including N. glabratus (C. glabrata), C. albicans and Candida parapsilosis. MIC and MFC tests revealed its inhibitory efficacy, while membrane permeability and ergosterol synthesis assays confirmed its mechanism of action, which compromises the structural integrity of fungal cells. Furthermore, 3g was effective both in preventing biofilm formation and in disrupting mature biofilms. Among the compounds evaluated, 3e and 3g demonstrated multiple mechanisms of action, which highlights promising characteristics for the development of new antifungal agents. In vivo studies are needed to validate the clinical use and evaluate the safety and effectiveness of the compounds.
- ItemFarinha de mosca soldado negro (Hermetia illucens), na nutrição do lambari (Astyanax bimaculatus)(Universidade Federal do Espírito Santo, 2024-09-27) Souza, Erivelto Oliveira de; Bolzan, Raphael Pires; https://orcid.org/0000-0002-3269-5180; http://lattes.cnpq.br/7880129709674385; Mendonça, Pedro Pierro; https://orcid.org/0000-0001-5128-0020; http://lattes.cnpq.br/3992381145892105; https://orcid.org/0000-0001-7212-1651; http://lattes.cnpq.br/6216330251223509; Vargas Junior, José Geraldo de; https://orcid.org/0000-0001-6801-5629; http://lattes.cnpq.br/6468487749303265; Gonçalves Junior, Lucas Pedro; https://orcid.org/0009-0000-2232-8443; http://lattes.cnpq.br/6111474381561776The growing global aquaculture production is increasing the demand for feed, with fishmeal being a notable but expensive component. Sustainable alternatives, such as black soldier fly (Hermetia illucens) meal, are being explored due to their efficiency and nutritional profile. This study nutritionally characterized black soldier fly meal in its various life stages and evaluated its effectiveness as an ingredient in diets for juvenile yellow tail-lambari (Astyanax bimaculatus). The nutritional characterization of the samples determined moisture, lipids, ash, crude protein, and gross energy content of the black soldier fly meals. The analyses were conducted in laboratories at IFES and UFES. Data were subjected to ANOVA and Tukey's test to check for significant differences. In performance experiments, the experimental feeds were formulated with levels of (T1 = control, T2 = 12.5%, T3 = 25%, T4 = 37.5%, and T5 = 50%) of black soldier fly meal from the pupal and adult stages. The fish were distributed through a completely randomized design, with 5 treatments and 4 replications, containing 10 fish per experimental unit, and the trial lasted 45 days. Statistical data were analyzed using ANOVA and polynomial regression. According to the nutritional characterization data, it can be deduced that the larval and pupal stages are the most suitable for use. The adult stage is less ideal due to the high chitin content. Performance data showed that diets replacing fishmeal with pupal black soldier fly meal yielded good results, indicating that this stage is usable. Conversely, diets containing adult black soldier fly meal did not perform well, likely due to excessive quitin.