Doutorado em Biotecnologia

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    Uso da gelatina fibrilar para hemostasia na neurocirurgia pediátrica
    (Universidade Federal do Espírito Santo, 2025-08-22) Colodetti, Rafael; Gouvea, Sônia Alves; https://orcid.org/0000-0001-5180-471X; http://lattes.cnpq.br/7268228122543743; https://orcid.org/0000-0001-7642-3702; http://lattes.cnpq.br/0217670945206928; Kusahara, Denise Miyuki; https://orcid.org/0000-0002-9498-0868; http://lattes.cnpq.br/2666393667209812; Bezerra, Itala Maria Pinheiro; https://orcid.org/0000-0002-8604-587X; https://lattes.cnpq.br/; Paula, Flavia de; https://orcid.org/0000-0001-8679-2982; https://lattes.cnpq.br/
    Introduction: Pediatric neurosurgery has peculiarities in that it treats patients with a variety of sizes and physical development, covering diseases that are sometimes non-existent among adults. These characteristics make them more susceptible to surgical complications due to low tolerance to bleeding. Therefore, the use of topical hemostatics is suitable for rapid control of hemorrhages in all surgical periods. Objective: To evaluate the feasibility, performance and safety of fibrillar gelatin-based hemostatic (HBGF) in pediatric neurosurgery. Material and Methods: The selection of the sample group was random, intentional and non-probabilistic. The performance assessment of the HBGF was carried out by applying a performance questionnaire containing a classification into 5 topics (excellent, good, satisfactory, moderate and poor), focusing on its characteristics in stopping bleeding, absorption capacity, general handling, adherence to the lesion and ease of repositioning. Clinical and neuroimaging parameters were monitored 24 hours and 30 to 60 days after the surgical approach. Appropriate statistical tests were used to analyze the data. Results: The sample was divided into 4 groups, with Group 1 (G1) open surgeries for brain tumor resection; Group 2 (G2) open surgeries, no brain tumor; Group 3 (G3) minimally invasive procedures; and, Group 4 (G4), tumors in the posterior fossa. The highest prevalence was between 2 and 5 years old, in girls and with total biodegradation of HBGF within 8 weeks. In all groups, the majority had a hemostasis time of “gelatin-based hemostatic in fibrillar presentation. This demonstrated feasibility, good performance and safety in several pediatric neurosurgery procedures.
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    Qualidade de vida de policiais militares : fatores determinantes e relação epigenética do gene BDNF
    (Universidade Federal do Espírito Santo, 2025-05-23) Moreno, Ivana Alece Arantes; Silva, Adriana Madeira Álvares da ; https://orcid.org/0000-0002-8078-0304; http://lattes.cnpq.br/6445492335035108; https://orcid.org/0000-0003-3407-4019; http://lattes.cnpq.br/4011255253507606; Errera, Flávia Imbriosi do Valle ; https://orcid.org/0000-0002-8069-6372; http://lattes.cnpq.br/9337327437538048; Freitas, Flávia Vitorino ; https://orcid.org/0000-0003-3722-9987; http://lattes.cnpq.br/0564698519017379; Sartório, Carmem Luiza ; https://orcid.org/0000-0002-2341-1596; http://lattes.cnpq.br/1299417616233163; Rocha, José Cláudio Casali da ; https://orcid.org/0000-0002-1838-2153; http://lattes.cnpq.br/6612912880886333
    In the context of the numerous challenges and high levels of stress routinely experienced by police officers, this study aims to investigate the association between sociodemographic factors, lifestyle, psychological aspects, and BDNF gene promoter methylation with the quality of life of these professionals. This is a cross-sectional study conducted with 166 police officers from the state of Espírito Santo, Brazil. Validated instruments were used to assess quality of life, symptoms of stress, depression, use of psychotropic medications, and other sociodemographic and lifestyle variables. Statistical analysis included regression multivariate models to identify predictors of quality of life domains and the association between quality of life and BDNF gene methylation. The results indicated that symptoms of stress and depression, use of anxiolytics and antidepressants, sex, and education level were significantly associated with poorer quality of life across different domains. Furthermore, higher levels of BDNF gene methylation were associated with lower scores in the psychological domain. These findings underscore the importance of interdisciplinary approaches to promoting mental health and quality of life among military police officers, as well as the potential use of epigenetic biomarkers in populations exposed to chronic stress conditions
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    Obtenção de peptídeos com atividades biológicas obtidas a partir das vísceras de tilápia (Oreochromis spp.)
    (Universidade Federal do Espírito Santo, 2025-06-27) Vasconcelos, Antônio Victor Baioco; Co-orientador1; Orientador1; https://orcid.org/; 1º membro da banca; 2º membro da banca; 3º membro da banca
    The intensive production of tilapia (Oreochromis spp.) in Brazil generates large volumes of organic waste, particularly viscera, which are rich in proteins and enzymes and hold biotechnological potential. This study aimed to obtain, fractionate, and characterize bioactive peptides from tilapia viscera, with an emphasis on antioxidant, antimicrobial, and toxicological activities, in alignment with principles of circular economy and sustainable development. Proteins were extracted using organic solvents, desalted by molecular exclusion chromatography (Sephadex G-25), and fractionated by molecular exclusion chromatography (Sephadex G-50), yielding seven peptide fractions (F1 to F7). The antioxidant activity was evaluated by the ABTS•⁺ radical scavenging assay, using Trolox as the standard. Fractions F4, F5, and F6 showed the highest antioxidant capacities (1933.4; 1520.5; and 1546.7 µmol·L⁻¹ Trolox equivalents, respectively), suggesting the presence of low molecular weight peptides enriched in amino acid residues such as tyrosine, tryptophan, and histidine. Toxicological evaluation was performed using larvae of Tenebrio molitor as an alternative in vivo model in compliance with the 3Rs principles. The fractions were injected at three different concentrations (10, 40, and 80 µg), and larval survival was monitored over 120 hours. A binomial generalized linear model (GLM) indicated dose- and time-dependent toxicity. Fractions F4, F5, and F6 were the most toxic (≥ 93.3 % mortality at 80 µg), while F2 showed the lowest toxicity, with mortality remaining below 40 % even at the highest dose. Conversely, none of the peptide fractions exhibited detectable antimicrobial activity against Staphylococcus aureus, Escherichia coli, or Pseudomonas aeruginosa under the disc diffusion assay, possibly due to the absence of bactericidal peptides or limitations in compound diffusion through the agar matrix. In conclusion, the tilapia viscera represent a promising source of antioxidant peptides, with fractions F4, F5, and F6 standing out as candidates for further development. The use of T. molitor proved to be a viable alternative model for preliminary toxicity screening. These findings support the valorization of aquaculture by-products as bioactive resources and provide a foundation for developing functional ingredients for the food, pharmaceutical, or cosmetic industries
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    Patogênese molecular da COVID-19: o envolvimento de fatores genéticos, hemostáticos e imunológicos na evolução de quadros graves, desenvolvimento de coagulopatias e COVID Longa.
    (Universidade Federal do Espírito Santo, 2025-03-21) Silva, Danielle Ribeiro Campos da Silva; Meira, Débora Dummer; https://orcid.org/0000-0002-6092-2459; http://lattes.cnpq.br/7199119599752978; Louro, Iúri Drumond; https://orcid.org/0000-0001-5160-9615; http://lattes.cnpq.br/3817361438227180; https://orcid.org/0009-0004-3821-7376; http://lattes.cnpq.br/8642525842646026; Paula, Flávia de; https://orcid.org/0000-0001-8679-2982; http://lattes.cnpq.br/7913201450663683; Figueiras, Paulo Roberto; https://orcid.org/0000-0002-6068-1982; ; Prezotti, Alessandra Nunes Loureiro; https://orcid.org/0000-0003-1079-7140; http://lattes.cnpq.br/9069306254016368; Pereira, Fausto Edmundo Lima; https://orcid.org/0000-0002-9219-3537; http://lattes.cnpq.br/4065537941002091
    Introduction: The development of the inflammatory process triggered by the SARS-CoV-2 virus leads to abnormal activation of the coagulation system, pathologically manifesting as generalized endothelial dysfunction in small vessels and microthrombosis in the alveolar region. Following the pandemic, persistent symptoms and sequelae are frequently associated with this infection—referred to as “Long COVID”—and generally have a negative impact on individuals’ daily activities. The investigation of coagulation-related markers, cytokines, antiphospholipid antibodies, and genotyping of interleukins and thrombophilia-associated genes that influence hyperinflammation, endothelial dysfunction, and hypoxia is highly relevant for the prognosis of surviving patients. Objective: To investigate molecular and genetic alterations in the hemostatic and inflammatory systems, ABO blood group, and the presence of acquired antibodies in patients who tested positive for COVID-19, as well as the association of these factors with disease severity, potential occurrence of thrombotic complications, and the development of Long COVID. Methods: A total of 226 unvaccinated volunteers (considering two doses) with RT-PCR-confirmed COVID-19 were recruited between November 2020 and December 2021. The cohort was divided into three groups: Mild (60), Moderate (65), and Severe (101), according to WHO guidelines. All participants signed an Informed Consent Form, completed a questionnaire, and had blood samples collected for analysis. The questionnaire assessed relevant preexisting clinical conditions, medical record data, symptoms, and the occurrence of sequelae. Laboratory tests were conducted to assess thrombosis, blood typing, hemostatic/inflammatory biomarkers, and genotyping. Participants whose test results were outside reference ranges were re-evaluated six months later, and after two years, they completed a follow-up questionnaire focused on Long COVID and quality of life. Appropriate statistical tests were used for data analysis. Results: A significant relationship was identified between disease severity and sociodemographic and biomarker data. Statistically significant factors included: age (P age (P<0.0001), lower educational level/ social vulnerability (P<0.0001), ethnicity (P=0.003), overweight (P<0.0001), MTHFR rs1801133 polymorphism (P=0.035), previous cardiovascular disease (P=0.002), diabetes mellitus (DM) (P=0.006), Factor VIII (FVIII) (P=0.046), von Willebrand Factor (vWF) (P=0.0020), and D-dimer (DD) (P<0.0001). After six months, FVIII (P=0.0005), vWF (P=0.0022), and DD (P<0.0001) levels significantly decreased, but only DD returned to normal levels.Sequelae affecting the musculoskeletal, cardiorespiratory, and vascular systems were investigated. Individuals with chronic pulmonary disease (CPD) and DM had higher chances of developing joint pain and myalgia. CPD was also associated with dyspnea and palpitations. Elevated DD levels contributed to palpitations. Prolonged dry cough was associated with lower education and non-white ethnicity. Women were more likely to develop phlebitis. FVIII was associated with a higher risk of cramps, and regular physical activity showed a protective effect against swelling. The EQ-5D-5L questionnaire assessed current quality of life, with the most severely affected participants reporting greater impairment across all measured domains. Conclusion: The findings suggest that endothelial dysfunction is a central factor and may contribute to the development of Long COVID.
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    Análise de custo do tratamento do câncer da cavidade oral e orofaringe: Uso do método de microcusteio por bottom-up para dados de um hospital de referência no Brasil
    (Universidade Federal do Espírito Santo, 2024-03-11) Zorzaneli, Willene dos Santos Machado; Zara, Ana Laura Amâncio de Sene; Zeidler, Sandra Ventorin von; https://orcid.org/0000-0002-8897-5747; http://lattes.cnpq.br/5785612863130498; Paula, Flavia de; Guimarães, Michele Ferreira; Silva, Everton Nunes da
    Cost analysis in OC/OPC is scarce, however, necessary since cost can be an essential tool for decision-making. This study aimed to estimate the direct costs of oral and oropharyngeal squamous cell carcinoma (OC/OPC) in a state in southeastern Brazil. To this end, a cost of illness study was carried out based on the bottom-up microcosting method as a partial economic assessment, considering the direct medical costs related to outpatients and inpatients from the perspective of reimbursement rates from the Unified Health System. Data were collected from the medical records of 41 randomized patients recruited, between 2013 and 2018. All cases were confirmed as squamous cell carcinoma of the mouth and oropharynx (C02 to C06, C09 and C10 according to the International Classification of Diseases for Oncology, 3rd Edition). Each cost value was adjusted for annual accumulated inflation. The statistical analysis of descriptive analyses, parametric tests, ANOVA and Student t test and non-parametric tests, Kruskal Wallis and Mann Whitney, was used using the SPSS® Statistical program. To adjust the differences in confidence intervals at 95%, the bootstrap method was used for 5.000 resamples using the Stata® statistical program. For all statistical tests, the significance level was set at 5%. Healthcare system cost estimates for 41 patients with OC/OPC amounted to a total cost of I$164,671.07 with an average of IS$4,042.5 per patient. 22,814 procedures and uses of health services were carried out. The sample was mostly made up of men (>80%) and aged between 51 and 60 years (41.5%). Carcinomas of the base of the tongue and floor of the mouth had the highest costs, I$4,288.25 and 6,586.88, respectively. Clinical stage III was the one with the most procedures and highest costs, I$5,415.11 (p<0.001) per patient. From the perspective of the Unified Health System, there is a high-cost demand for OC/OPC treatment, especially in advanced stages of the disease. This study contributes to decision- making regarding treatment as well as the implementation of programs aimed at early detection of diseases