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- ItemEstudos sobre a diferenciação de sinais transitórios de fluorescência da clorofila a em plantas de mamoeiro com e sem sintomas na presença dos vírus PRSV-P e PMeV(Universidade Federal do Espírito Santo, 2025-11-30) Medeiros, Weverton Pereira de; Silva, Diolina Moura; https://orcid.org/0000-0003-3885-280X; https://lattes.cnpq.br/; https://orcid.org/0000-0001-9193-894X; https://lattes.cnpq.br/; Paula, Flávia de; https://orcid.org/0000-0001-8679-2982; https://lattes.cnpq.br/; Gouvea, Sônia Alves; https://orcid.org/0000-0001-5180-471X; https://lattes.cnpq.br/; Nascimento, Vitor de Laia; https://orcid.org/0000-0001-5276-2638; https://lattes.cnpq.br/; Costa, Lucas Calvacante da; https://orcid.org/0000-0002-4891-9185; https://lattes.cnpq.br/Infecções virais representam uma ameaça crítica às espécies de plantas cultivadas. No cultivo de mamão, duas doenças virais mosaico do mamão (causada pelo vírus da mancha anelar do mamão tipo P PRSV-P) e doença pegajosa do mamão (causada por um complexo viral do vírus da meleira do mamão PMeV e do vírus da meleira do mamão PMeV2) são prevalentes e capazes de devastar plantações inteiras, incorrendo em perdas econômicas substanciais. As práticas atuais de diagnóstico dependem da identificação visual dos sintomas e da eliminação de plantas infectadas (roguing). O monitoramento da eficiência fotossintética em pomares propensos à coinfecção por PRSV-P e PMeV2 pode permitir uma intervenção precoce, mitigando perdas de produtividade e reduzindo a qualidade dos frutos. Este estudo teve como objetivo avaliar a fluorescência da clorofila a como um biomarcador para comprometimento fotossintético e severidade dos sintomas em mamão infectado com PRSV-P e/ou PMeV2 e explorar a viabilidade da detecção precoce da infecção por esses patógenos duplos, como um estudo exploratório em condições de campo. A fluorescência da clorofila a revelou detalhes sobre a fisiologia de plantas coinfectadas com o complexo de PMeV2 e PRSV-P: a força motriz eletrônica dentro do fotossistema II (FSII) diminui em plantas infectadas e naquelas sem sintomas visuais de infecção, sendo proporcional à idade e ao estágio de desenvolvimento das plantas. Uma desaceleração na rotatividade de transferência de múltiplos elétrons do FSII e uma diminuição na eficiência das reações redox do fotossistema I foram observadas em plantas com ou sem detecção visual da infecção. As evidências geradas sugerem que a técnica de fluorescência da clorofila a pode ser usada para monitorar o estado fisiopatológico de plantas sob estresse biótico.
- ItemNovo epóxi para plastinação: análise de viabilidade e aplicabilidade na conservação de tecidos biológicos(Universidade Federal do Espírito Santo, 2025-11-24) Monteiro, Yuri Favalessa; Bittencourt, Athelson Stefanon; https://orcid.org/0000-0003-1378-2577; http://lattes.cnpq.br/3498043196182770; Nogueira, Breno Valentim; https://orcid.org/0000-0002-2199-0635; http://lattes.cnpq.br/0011229320439147; https://orcid.org/0000-0001-8521-5593; http://lattes.cnpq.br/7269203136333787; Guimarães, Marco Cesar Cunegundes; https://orcid.org/0000-0003-2146-0180; http://lattes.cnpq.br/0261991057482057; Soares, Kinglston; https://orcid.org/0000-0003-2669-391X; http://lattes.cnpq.br/7671657131724834 ; Carretta Júnior, Moacir; https://orcid.org/0000-0002-5959-9105; http://lattes.cnpq.br/4748325408764080 ; Baptista, Carlos Augusto de Camargo Souza; http://lattes.cnpq.br/3554852890141199Plastination is a technique for preserving biological tissues, the principle of which is the replacement of bodily fluids with a curable polymer. The polymer classes most commonly used in plastination are silicone and polyester and epoxy resins. Epoxy is utilized in 2- to 5-millimeter-thick sections of biological tissues or anatomical segments, with the German resin Biodur® E12 being the most well-known and globally used. There are few alternative epoxies to the reference one (E12) for use in the technique. The objective of this work was to apply a commercial epoxy resin formulation with potential as an alternative to E12 and its curing system in the plastination process of thin sections of biological tissues. The methodology was divided into prospecting for a nationally available resin, adapting the formulation, applying the formulation in the plastination of Wistar rat sections, characterizing the resin, and evaluating the final specimens. From a market search, the E48 epoxy (brand not disclosed) was found, which met the initial prerequisites for testing, and its formulation was altered with the addition of a plasticizer for use in the forced impregnation step. A total of 200 Wistar rat transverse sections were plastinated with the control (E12) and test (E48) polymers in 4 distinct replicates. The results of the shrinkage analysis show no statistical differences between the E12 and E48P resins (with plasticizer, developed in this research). In general, images taken with a confocal microscope showed greater sharpness and contrast for the sections of the E48P formulation. Regarding the characterization of the resins, the E48P formulation stands out for a much lower viscosity compared to the other epoxies tested, resulting from the presence of 35% of plasticizer in its formulation. Additionally, it showed greater flexibility, a faster yellowing rate, and a lower refractive index. Based on the results of the shrinkage analysis, confocal and stereoscopic microscopy, and characterization, it was concluded that the E48P epoxy is a viable alternative to E12.
- ItemUso da gelatina fibrilar para hemostasia na neurocirurgia pediátrica(Universidade Federal do Espírito Santo, 2025-08-22) Colodetti, Rafael; Gouvea, Sônia Alves; https://orcid.org/0000-0001-5180-471X; http://lattes.cnpq.br/7268228122543743; https://orcid.org/0000-0001-7642-3702; http://lattes.cnpq.br/0217670945206928; Kusahara, Denise Miyuki; https://orcid.org/0000-0002-9498-0868; http://lattes.cnpq.br/2666393667209812; Bezerra, Itala Maria Pinheiro; https://orcid.org/0000-0002-8604-587X; https://lattes.cnpq.br/; Paula, Flavia de; https://orcid.org/0000-0001-8679-2982; https://lattes.cnpq.br/Introduction: Pediatric neurosurgery has peculiarities in that it treats patients with a variety of sizes and physical development, covering diseases that are sometimes non-existent among adults. These characteristics make them more susceptible to surgical complications due to low tolerance to bleeding. Therefore, the use of topical hemostatics is suitable for rapid control of hemorrhages in all surgical periods. Objective: To evaluate the feasibility, performance and safety of fibrillar gelatin-based hemostatic (HBGF) in pediatric neurosurgery. Material and Methods: The selection of the sample group was random, intentional and non-probabilistic. The performance assessment of the HBGF was carried out by applying a performance questionnaire containing a classification into 5 topics (excellent, good, satisfactory, moderate and poor), focusing on its characteristics in stopping bleeding, absorption capacity, general handling, adherence to the lesion and ease of repositioning. Clinical and neuroimaging parameters were monitored 24 hours and 30 to 60 days after the surgical approach. Appropriate statistical tests were used to analyze the data. Results: The sample was divided into 4 groups, with Group 1 (G1) open surgeries for brain tumor resection; Group 2 (G2) open surgeries, no brain tumor; Group 3 (G3) minimally invasive procedures; and, Group 4 (G4), tumors in the posterior fossa. The highest prevalence was between 2 and 5 years old, in girls and with total biodegradation of HBGF within 8 weeks. In all groups, the majority had a hemostasis time of “gelatin-based hemostatic in fibrillar presentation. This demonstrated feasibility, good performance and safety in several pediatric neurosurgery procedures.
- ItemQualidade de vida de policiais militares : fatores determinantes e relação epigenética do gene BDNF(Universidade Federal do Espírito Santo, 2025-05-23) Moreno, Ivana Alece Arantes; Silva, Adriana Madeira Álvares da ; https://orcid.org/0000-0002-8078-0304; http://lattes.cnpq.br/6445492335035108; https://orcid.org/0000-0003-3407-4019; http://lattes.cnpq.br/4011255253507606; Errera, Flávia Imbriosi do Valle ; https://orcid.org/0000-0002-8069-6372; http://lattes.cnpq.br/9337327437538048; Freitas, Flávia Vitorino ; https://orcid.org/0000-0003-3722-9987; http://lattes.cnpq.br/0564698519017379; Sartório, Carmem Luiza ; https://orcid.org/0000-0002-2341-1596; http://lattes.cnpq.br/1299417616233163; Rocha, José Cláudio Casali da ; https://orcid.org/0000-0002-1838-2153; http://lattes.cnpq.br/6612912880886333In the context of the numerous challenges and high levels of stress routinely experienced by police officers, this study aims to investigate the association between sociodemographic factors, lifestyle, psychological aspects, and BDNF gene promoter methylation with the quality of life of these professionals. This is a cross-sectional study conducted with 166 police officers from the state of Espírito Santo, Brazil. Validated instruments were used to assess quality of life, symptoms of stress, depression, use of psychotropic medications, and other sociodemographic and lifestyle variables. Statistical analysis included regression multivariate models to identify predictors of quality of life domains and the association between quality of life and BDNF gene methylation. The results indicated that symptoms of stress and depression, use of anxiolytics and antidepressants, sex, and education level were significantly associated with poorer quality of life across different domains. Furthermore, higher levels of BDNF gene methylation were associated with lower scores in the psychological domain. These findings underscore the importance of interdisciplinary approaches to promoting mental health and quality of life among military police officers, as well as the potential use of epigenetic biomarkers in populations exposed to chronic stress conditions
- ItemObtenção de peptídeos com atividades biológicas obtidas a partir das vísceras de tilápia (Oreochromis spp.)(Universidade Federal do Espírito Santo, 2025-06-27) Vasconcelos, Antônio Victor Baioco; Co-orientador1; Orientador1; https://orcid.org/; 1º membro da banca; 2º membro da banca; 3º membro da bancaThe intensive production of tilapia (Oreochromis spp.) in Brazil generates large volumes of organic waste, particularly viscera, which are rich in proteins and enzymes and hold biotechnological potential. This study aimed to obtain, fractionate, and characterize bioactive peptides from tilapia viscera, with an emphasis on antioxidant, antimicrobial, and toxicological activities, in alignment with principles of circular economy and sustainable development. Proteins were extracted using organic solvents, desalted by molecular exclusion chromatography (Sephadex G-25), and fractionated by molecular exclusion chromatography (Sephadex G-50), yielding seven peptide fractions (F1 to F7). The antioxidant activity was evaluated by the ABTS•⁺ radical scavenging assay, using Trolox as the standard. Fractions F4, F5, and F6 showed the highest antioxidant capacities (1933.4; 1520.5; and 1546.7 µmol·L⁻¹ Trolox equivalents, respectively), suggesting the presence of low molecular weight peptides enriched in amino acid residues such as tyrosine, tryptophan, and histidine. Toxicological evaluation was performed using larvae of Tenebrio molitor as an alternative in vivo model in compliance with the 3Rs principles. The fractions were injected at three different concentrations (10, 40, and 80 µg), and larval survival was monitored over 120 hours. A binomial generalized linear model (GLM) indicated dose- and time-dependent toxicity. Fractions F4, F5, and F6 were the most toxic (≥ 93.3 % mortality at 80 µg), while F2 showed the lowest toxicity, with mortality remaining below 40 % even at the highest dose. Conversely, none of the peptide fractions exhibited detectable antimicrobial activity against Staphylococcus aureus, Escherichia coli, or Pseudomonas aeruginosa under the disc diffusion assay, possibly due to the absence of bactericidal peptides or limitations in compound diffusion through the agar matrix. In conclusion, the tilapia viscera represent a promising source of antioxidant peptides, with fractions F4, F5, and F6 standing out as candidates for further development. The use of T. molitor proved to be a viable alternative model for preliminary toxicity screening. These findings support the valorization of aquaculture by-products as bioactive resources and provide a foundation for developing functional ingredients for the food, pharmaceutical, or cosmetic industries