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INFLUÊNCIA MITOCONDRIAL NA REGULAÇÃO DE GENES ENVOLVIDOS COM ENVELHECIMENTO EM Saccharomyces cerevisiae SUBMETIDAS À ALTA PRESSÃO HIDROSTÁTICA
(Universidade Federal do Espírito Santo, 2020-02-07) Spagnol, Brigida de Almeida Amorim; Fernandes, Patricia Machado Bueno; https://orcid.org/0000000326953638; http://lattes.cnpq.br/2441925791593067; https://orcid.org/; http://lattes.cnpq.br/; Lomeli, Monica Montero; https://orcid.org/; http://lattes.cnpq.br/; Fernandes, Antonio Alberto Ribeiro; https://orcid.org/; http://lattes.cnpq.br/4696507759154477; Santos, Alexandre Martins Costa; https://orcid.org/0000000288018875; http://lattes.cnpq.br/4144105396879016; Pinto, Nadja Cristhina de Souza
Saccharomyces cerevisiae is a well-accepted eukaryotic model organism for studies on stress response and aging, WHEREas preserves several biochemical, molecular and organelle functioning mechanisms. Mitochondria is an organelle involved with stress respon
Fluorescência da clorofila a como indicador de porta enxertos de manga ‘Ubá’ mais tolerantes ao déficit hídrico
(Universidade Federal do Espírito Santo, 2020-02-13) Silva, Leonardo Faria; Silva, Diolina Moura; https://orcid.org/000000033885280X; http://lattes.cnpq.br/0341541450627705; https://orcid.org/0000000192822818; http://lattes.cnpq.br/; Santos, Alexandre Martins Costa; https://orcid.org/0000000288018875; http://lattes.cnpq.br/4144105396879016; Araujo, Wagner Luiz; https://orcid.org/; http://lattes.cnpq.br/; Ventura, Jose Aires; https://orcid.org/0000000314221739; http://lattes.cnpq.br/8687116881326074; Nesi, Adriano Nunes
In order to select the photochemical parameters extracted from the chlorophyll a fluorescence kinetics as being the simplest, fastest and most efficient for early identification of the most vigorous rootstock in the formation of drought tolerant 'Ubá' see
INFECÇÃO POR PAPILOMAVÍRUS HUMANO E EXPRESSÃO DAS PROTEÍNAS P16 E EGFR COMO BIOMARCADORES DE PROGNÓSTICO EM CARCINOMA EPIDERMOIDE ORAL E OROFARÍNGEO
(Universidade Federal do Espírito Santo, 2020-03-30) Abreu, Priscila Marinho de; Zeidler, Sandra Lucia Ventorin Von; https://orcid.org/0000000288975747; http://lattes.cnpq.br/5785612863130498; https://orcid.org/0000000164537171; http://lattes.cnpq.br/; Rotta, Rejane Faria Ribeiro; https://orcid.org/; http://lattes.cnpq.br/; Rabenhorst, Silvia Helena Barem; https://orcid.org/; http://lattes.cnpq.br/; Paneto, Greiciane Gaburro; https://orcid.org/0000000180354199; http://lattes.cnpq.br/8176374147579841; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; Podesta, Jose Roberto Vasconcelos de
Oral and oropharyngeal squamous cell carcinoma has as main risk factors the consumption of tobacco, alcohol and infection by Human Papillomavirus (HPV). Studies indicate that HPV-positive patients have results more favorable in relation to survival and re
Sazonalidade, epidemiologia molecular e virulência do Vírus Sincicial Respiratório (VSR): uma perspectiva dentro do programa brasileiro de vigilância da influenza
(Universidade Federal do Espírito Santo, 2021-05-24) Vianna, Lucas Alves; Louro, Iuri Drumond; https://orcid.org/0000000151609615; http://lattes.cnpq.br/3817361438227180; https://orcid.org/0000-0001-8487-9929; http://lattes.cnpq.br/6931449640908148; Arruda Neto, Eurico de; https://orcid.org/0000-0002-0978-410X; http://lattes.cnpq.br/1632065399563444; Fernandes, Patricia Machado Bueno; https://orcid.org/0000000326953638; http://lattes.cnpq.br/2441925791593067; Spano, Liliana Cruz; https://orcid.org/0000000262056988; http://lattes.cnpq.br/7451382455806895; Silva, Paola Cristina Resende; https://orcid.org/0000-0002-2884-3662; http://lattes.cnpq.br/3725179069720464; Sousa, Rita Catarina Medeiros; https://orcid.org/0000-0002-8330-6667; http://lattes.cnpq.br/3560941703812539
Respiratory Syncytial Virus (RSV) is the main cause of pediatric morbidity and mortality. The complex evolution of RSV creates a need for worldwide surveillance, which may assist in the understanding of multiple viral aspects. This study aimed to investigate RSV features under the Brazilian Influenza Surveillance Program, evaluating the role of viral load and genetic diversity in disease severity and the influence of climatic factors in viral seasonality. We have investigated the prevalence of RSV in children up to 3 years old with severe acute respiratory infection (SARI) in the Espirito Santo State (ES), Brazil, from 2016 to 2018. RT-qPCR allowed for viral detection and viral load quantification, to evaluate association with clinical features and mapping of local viral seasonality. Gene G sequencing and phylogenetic reconstruction demonstrated local genetic diversity. Of 632 evaluated cases, 56% were caused by RSV, with both subtypes A and B co-circulating throughout the years. A discrete inverse association between average temperature and viral circulation was observed. No correlation between viral load and disease severity was observed, but children infected with RSV-A presented higher clinical severity score (CSS) median, stayed longer in the hospital, required intensive care and ventilatory support more frequently than those infected by RSV-B. Regarding RSV diversity, some local genetic groups were observed in the main genotypes circulation RSV-A ON1 and RSV-B BA, with strains showing modifications in the G gene amino acid chain. Local RSV studies using the Brazilian Influenza Surveillance Program are relevant to assess the suitability and viability of a single network for the surveillance of other respiratory viruses. Understanding seasonality, virulence and genetic diversity can support the suitability of future antiviral drugs and vaccines and assist in the administration of prophylactic strategies.
ANÁLISE DA EXCITABILIDADE E CONECTIVIDADE CORTICAL PARA UM SISTEMA DE NEURORREABILITAÇÃO BASEADO EM INTERFACE-CÉREBRO MÁQUINA E MONOCICLO ROBÓTICO
(Universidade Federal do Espírito Santo, 2021-06-29) Cardoso, Vivianne Flavia; Filho, Teodiano Freire Bastos; https://orcid.org/0000000211852773; http://lattes.cnpq.br/3761585497791105; https://orcid.org/; http://lattes.cnpq.br/; Nogueira, Breno Valentim; https://orcid.org/0000000221990635; http://lattes.cnpq.br/0011229320439147; Andrade, Adriano de Oliveira; https://orcid.org/; http://lattes.cnpq.br/; Caldeira, Eliete Maria de Oliveira; https://orcid.org/; http://lattes.cnpq.br/; Neto, Anselmo Frizera; https://orcid.org/0000000206873967; http://lattes.cnpq.br/8928890008799265; Rodriguez, Denis Delisle; https://orcid.org/000000028937031X; http://lattes.cnpq.br/7140331839822423
CARDOSO V.F. 2021. 110f. Analysis of excitability and connectivity cortical for a neurorehabilitation system based on brain machine interface and robotic monocycle. 2021. 121f. Thesis (doctoral in biotechnology)- Postgraduation Biotechnological Program, U
Estudo de associação das variantes dos genes TCF7L2 e ADIPOQ com diabetes, obesidade e longevidade em uma coorte de idosos de São Paulo
(Universidade Federal do Espírito Santo, 2021-08-11) Bride, Lais de Lima; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; https://orcid.org/0000-0003-3703-2631; http://lattes.cnpq.br/4221199792517120; Bastos Filho, Teodiano Freire; https://orcid.org/0000000211852773; http://lattes.cnpq.br/3761585497791105; Maranduba, Carlos Magno da Costa; https://orcid.org/0000-0001-7327-1934; http://lattes.cnpq.br/4763153859701731; Errera, Flavia Imbroisi Valle; https://orcid.org/0000-0002-8069-6372; http://lattes.cnpq.br/9337327437538048; Paneto, Greiciane Gaburro; https://orcid.org/0000000180354199; http://lattes.cnpq.br/8176374147579841; Luizon, Marcelo Rizzatti; https://orcid.org/0000-0002-8331-3525; http://lattes.cnpq.br/1264026443614775
Type 2 diabetes mellitus (DM2) and obesity are complex diseases directly related to the quality and expectancy of life of elderly across the world in the current century. There are several reports that genetic factors play a crucial role in the risk for complex diseases. Therefore, the investigation of genetic biomarkers is an important biotechnological tool for the prevention and treatment of complex diseases, also for better healthy aging and lifespan. The TCFL72 and ADIPOQ genes encode important proteins participating in metabolic processes and these genes have been widely described by association studies. Thus, the main objectives of this study were to evaluate the association of the rs7903146 polymorphism in TCF7L2 gene with DM2 and obesity, as also the relation of the SNPs rs17300539 (-11391G/A), rs266729 (- 11377G/C), rs2241766 (+45T/G) and rs1501299 (+276 G/T) in ADIPOQ gene with longevity status. More than 1,000 participants from an elderly cohort named SABE (Saúde, Bem Estar e Envelhecimento - Health, Welfare and Aging) were evaluated over 10 years (2000 to 2010) and the anthropometric, clinical and genetic data were collected during this period. The TCF7L2 and ADIPOQ genotypes were extracted from the ABraOM (Online Archive of Brazilian Mutations) database. Logistic regression models and additional statistical analysis were performed and the main findings were: the association of TCF7L2 rs7903146 T allele with the risk for DM2 in inverse relation to the BMI status; the TCF7L2 rs7903146 C allele increased the risk for obesity; Association of the ADIPOQ variants rs2241766 with longevity and rs1501299 with protection for longevity; The “T/T” haplotype from rs2241766 and rs1501299 variants showed association with less chance for longevity, whereas the “G/G” haplotype from the same variants was positively associated with longevity risk. When gender was considered, we detected stronger associations of rs7903146 SNP with diabetes in men, while more significant values concerning the association of ADIPOQ variants with longevity were observed in women. Thus, the present study confirms the TCF7L2 rs7903146 SNP association with diabetes and obesity and that ADIPOQ variants are associated with longevity status in our studied cohort.
FATORES DETERMINANTES DA DOR CRÔNICA E O PAPEL DA METILAÇÃO DO GENE NR3C1
(Universidade Federal do Espírito Santo, 2021-08-30) Branco, Alexandre Lima Castelo; Louro, Iuri Drumond; https://orcid.org/0000000151609615; http://lattes.cnpq.br/3817361438227180; https://orcid.org/0000-0002-1704-9877; http://lattes.cnpq.br/7332472547330240; Guimarães, Marco Cesar Cunegundes; https://orcid.org/0000000321460180; http://lattes.cnpq.br/0261991057482057; Arantes, Lidia Maria Rebolho Batista; https://orcid.org/0000-0001-8230-1218; http://lattes.cnpq.br/2019308149950531; Carvalho, Marcos Brasilino de; https://orcid.org/0000-0001-6854-2680; http://lattes.cnpq.br/6208433886573740; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108
Chronic pain is a multidimensional health condition with high prevalence in Brazil and its chronic condition may be related to depression and anxiety, diseases recognized as the most prevalent mental disorders in the world and major causes of functional incapacity, suffering and reduced quality of life. The relationship between depression, anxiety, pain, suffering and epigenetic alterations have already been described in the literature, but this relationship is not completely clear yet. Epigenetic alterations can affect gene expression and are related to the individual's adaptation to the environment in a relationship between genotype, phenotype and environment. The glucocorticoid receptor gene, NR3C1, is regulated by epigenetic mechanisms and acts to control the neuroendocrine axis via cortisol, which also links the gene to depression and other psychiatric illnesses. Thus, this research evaluated the determinants of chronic pain, biopsychosocial, biochemical and molecular factors in the epigenetic modifications of the NR3C1 gene in adults aged between 20 and 59 years, users of the Brazilian Unified Health System. The results of the biopsychosocial assessment in the sample showed a profile of people over 40 years old, with lower per capita income and education, low levels of cortisol, more reports of stress and anxiety, higher consumption of continuous medications, less physical activity and more prevalence of chronic pain. Pain was related to a statistical model that pointed out pain-related variables. Thus, the research showed indicators that point to a predominant profile of individuals with chronic pain, with determinant factors being: hypomethylation of the DNA of the NR3C1 gene in CpG 42, age over 40 years and low cortisol.
Variabilidade genética em NOTCH1: associação com o excesso de peso e implicações terapêuticas na Leucemia Linfocítica Crônica em uma coorte de idosos
(Universidade Federal do Espírito Santo, 2021-09-03) Barcelos, Estevão Carlos Silva; Zeidler, Sandra Lúcia Ventorin Von; https://orcid.org/0000000288975747; http://lattes.cnpq.br/5785612863130498; https://orcid.org/0000-0001-7076-6165; http://lattes.cnpq.br/6899125943013073; Maranduba, Carlos Magno da Costa; https://orcid.org/0000-0001-7327-1934; http://lattes.cnpq.br/4763153859701731; Naslavsky, Michel Satya; https://orcid.org/0000-0002-9068-1713; http://lattes.cnpq.br/5084104783599564; Nogueira, Breno Valentim; https://orcid.org/0000-0002-2199-0635; http://lattes.cnpq.br/0011229320439147; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683
NOTCH1 is critical for the maintenance of stem cells and adult tissues, and appears to play as a key molecule in aging and in a variety of chronic conditions, including obesity and cancer, especially Chronic Lymphocytic Leukemia (CLL). Recently, the NOTCH1 signaling pathway has been identified as fundamental in adipocyte homeostasis, in addition to being directly linked to the inflammatory process. Furthermore, constitutive activation of NOTCH1 signaling is associated with poor prognosis in some types of cancer such as CLL where recurrent NOTCH1 mutations are responsible for stabilizing the signaling state. Given the involvement of the NOTCH1 gene in cell metabolism and development, the hypothesis of the present study is that genetic variations of NOTCH1 are associated with increased risk for overweight (overweight and obesity) and also alter the response of CLL cells to induced-stress. Therefore, as a first objective, we investigated whether genetic variations in NOTCH1 are associated with overweight/obesity in a Brazilian elderly cohort. To assess the association of NOTCH1 polymorphisms and overweight/obesity, anthropometric, biochemical, medical history, and lifestyle data were collected. Sequencing data from 1.024 individuals (aged 59 to 99 years) were analyzed: 424 were overweight, 320 were obese, and 280 were normal weight. We analyzed one hundred and sixty-one tag SNPs spanning the entire NOTCH1 gene and borders using the SNP tagging approach (minor allele frequency≥0.01 and pairwise linkage disequilibrium r2≥0.8). We observed the association of SNP rs9411207 with risk of overweight/obesity under the additive model, and the genotype distribution showed an increased frequency of homozygous TT (OR = 1.50, 95% CI: 1.20-1.88; P = 0.0002). The GAT haplotype constructed from this and other SNPs in high linkage disequilibrium (LD) was more frequent in overweight/obese subjects (P = 0.003). In silico analysis suggested that these SNPs likely affect the transcription of NOTCH1 and other genes. This is the first study reporting the association between NOTCH1 SNPs and risk of overweight/obesity. Considering the possibility of modulation of NOTCH1, additional population studies are needed to replicate these results and confirm the usefulness of these risk genotypes for new therapeutic strategies. A second objective was to evaluate whether the mutation in the NOTCH1 gene and the deregulation of the NOTCH1 pathway in CLL cases alter the response to curcumin. For this, we investigated the anti-CLL effect of curcumin and its ability to interfere in the integrated stress response (ISR) and in the NOTCH1 signaling pathway in primary CLL cells with mutation in the NOTCH1 gene and in the murine model of CLL Eμ-TCL1. Initially, gene expression data from cells with c.7544- 7545 delCT mutation in NOTCH1 were analyzed against data from Wild-Type (WT) cells. In silico analysis of gene expression of CLL cells mutated in NOTCH1 showed an increase in the expression of genes involved in Endoplasmic Reticulum (ER) and ISR stress. Primary CLL cells were collected and cultured with curcumin, a natural compound that also targets ER stress, for further analysis by Western blotting and real-time PCR. After in vitro exposure to curcumin, stress-related mechanisms were observed in primary cells with mutated NOTCH1 compared to WT cells. This response was preceded by an early increase in Ca2+ in the cytoplasm of CLL cells mutated in NOTCH1, which can trigger and maintain ER stress. Furthermore, curcumin increased the apoptosis of CLL cells carrying the NOTCH1 mutation, regardless of allelic load, when compared to WT cells. NOTCH1 signaling also appears modulated after exposure to curcumin. In particular, CLL cells mutated in NOTCH1 showed a reduction in the active portion of NOTCH1 (ICN1) and in the antiapoptotic proteins of the BCL2 family (BCL2 and MCL1), and the combination of curcumin with venetoclax, an anti-BCL2, shown to have a synergistic effect on CLL cells. In addition, we used the murine model Eμ-TCL1 to evaluate treatment with curcumin. In vivo administration of curcumin in the Eμ-TCL1 model significantly reduced the percentage of CD5+/CD19+ leukemic cells infiltrating the liver, spleen and bone marrow, with concomitant inhibition of NOTCH1 signaling in leukemic cells in the bone marrow. Our results suggest that ER stress-induced ISR activation and NOTCH1 signaling inhibition converge to amplification of CLL cell death and provide a crucial target for CLL treatment.
Novas abordagens no diagnóstico não invasivo de câncer bucal utilizando espectroscopia ftir em amostras de sangue e saliva
(Universidade Federal do Espírito Santo, 2021-09-29) Thebit, Marcela Marçal; Gouvea, Sonia Alves; https://orcid.org/000000015180471X; http://lattes.cnpq.br/7268228122543743; https://orcid.org/0000-0002-7305-5753; http://lattes.cnpq.br/7117284804597883; Abreu, Gláucia Rodrigues de; https://orcid.org/0009-0008-8772-8470; http://lattes.cnpq.br/0229590907405570; Endlich, Patrick Wander; https://orcid.org/0000-0002-7597-3603; http://lattes.cnpq.br/9669721267985878; Carvalho, Luis Felipe das Chagas e Silva de; https://orcid.org/0000-0003-1063-4624; http://lattes.cnpq.br/5669726117269568; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108
Oral cancer is usually diagnosed through invasive methods, for although systemic changes can be easily detected, the identification of an effective systemic biomarker using a simple and affordable approach has yet to take place. Attenuated total reflection Fourier transform infrared (ATR-FTIR) appears as an alternative for the chemical and structural characterization of organic and inorganic materials, including liquid samples as serum, plasma and saliva and a possible bridge between molecular analysis and clinical practice. Objective: The aim of this study was to evaluate if ATR-FTIR spectroscopy can be applied as a tool in oral cancer screening through the analysis of plasma and salivary samples. Methodology: Plasma and saliva samples from oral cancer patients and controls were analyzed by ATR-FTIR spectroscopy. Experimental data were fed into the MATLAB 2018b software and them analyzed through two different routines in order to identify points of differentiations between the groups. Results: Biochemical analysis of the plasma samples through ATR-FTIR yielded 92% sensitivity and 100% specificity in differentiating between groups. The biggest difference was found between peaks that might be related to nucleic acids and nucleic acid phosphate. Pilot study with salivary samples reached similar accuracy Conclusion: We conclude that ATR-FTIR successfully discriminated between oral cancer patients and healthy subjects using plasma samples, with nucleic acids being found as major biomarkers.
Dynamics of the papaya meleira virus complex during the development of papaya (Carica papaya L.)
(Universidade Federal do Espírito Santo, 2021-11-11) Araujo, Marlonni Maurastoni; Fernandes, Patricia Machado Bueno; https://orcid.org/0000000326953638; http://lattes.cnpq.br/2441925791593067; https://orcid.org/0000000260643126; http://lattes.cnpq.br/; Aragao, Francisco Jose Lima; https://orcid.org/; http://lattes.cnpq.br/; Fernandes, Antonio Alberto Ribeiro; https://orcid.org/; http://lattes.cnpq.br/4696507759154477; Junior, Francisco Murilo Zerbini; https://orcid.org/; http://lattes.cnpq.br/; Rodrigues, Silas Pessini; Withfield, Anna Elizabeth
MAURASTONI, M. A. Dynamics of the papaya meleira virus complex during the development of papaya (Carica papaya L.). 2021. 173p. Thesis for the Degree of Ph.D. in Biotechnology Postgraduation Biotechnology Programme, UFES, Espirito Santo. Brazil. Among t
“CARACTERIZAÇÃO DO PADRÃO DE MUTAÇÕES DA OSTEOGÊNESES IMPERFEITA E AVALIAÇÃO IN VITRO DOS EFEITOS DE ANTIOXIDANTES RESVERATROL, ÁCIDO ASCÓRBICO E CURCUMINA EM CÉLULAS-TRONCO MESENQUIMAIS”
(Universidade Federal do Espírito Santo, 2022-02-03) Trancozo, Maira; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; https://orcid.org/0000000185623482; http://lattes.cnpq.br/; Batitucci, Maria do Carmo Pimentel; https://orcid.org/; http://lattes.cnpq.br/0010148251489155; Maranduba, Carlos Magno da Costa; https://orcid.org/; http://lattes.cnpq.br/; Meira, Debora Dummer; https://orcid.org/; http://lattes.cnpq.br/; Nishimura, Agnes Lumi
Osteogenesis Imperfecta (OI) is an inherited disorder of connective tissues that contain collagen in their formation. More than 20 OI-related genes have been reported in the last years. Most cases happen due mutations inherited with autossomic dominant in
Valor prognóstico do infiltrado linfocitário tumoral e da expressão de PLK1 e FOXM1 em carcinoma epidermoide oral
(Universidade Federal do Espírito Santo, 2022-07-25) Damasceno, Thabata Coeli Dias; Zeidler, Sandra Lucia Ventorin Von; https://orcid.org/0000000288975747; http://lattes.cnpq.br/5785612863130498; https://orcid.org/0000000244434025; http://lattes.cnpq.br/; Mendonca, Elismauro Francisco de; https://orcid.org/; http://lattes.cnpq.br/; Lima, Sheila Coelho Soares; https://orcid.org/; http://lattes.cnpq.br/; Peterle, Gabriela Tonini; https://orcid.org/; http://lattes.cnpq.br/; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108
abstract
Identificação de cochonilhas em cafeeiros utilizando DNA Barcoding e High Resolution Melting e distribuição potencial de Planococcus citri no Espírito Santo
(Universidade Federal do Espírito Santo, 2023-01-26) Oliveira, Pablo Viana; Paneto, Greiciane Gaburro; https://orcid.org/0000000180354199; http://lattes.cnpq.br/8176374147579841; https://orcid.org/000000021785178X; http://lattes.cnpq.br/9623188128443119; Serrão, José Eduardo; https://orcid.org/0000-0002-0477-4252; http://lattes.cnpq.br/6663553463256293; Telles, Mariana Pires de Campos; https://orcid.org/0000-0002-9023-0007; http://lattes.cnpq.br/4648436798023532; Ferreira, Marcia Flores da Silva; https://orcid.org/0000-0003-1541-6634; http://lattes.cnpq.br/5719813884063445; Ventura, José Aires; https://orcid.org/0000000314221739; http://lattes.cnpq.br/8687116881326074
Mealybugs (Hemiptera: Pseudococcidae) are agricultural pests of several crops, which can transmit viruses and cause great economic losses. For the control and management of these pests is essential a rapid and accurate identification of these insects. In this study, a rapid and low-cost method for identifying mealybug species was developed. The objectives are presented in the Results and Discussion section written in three chapters in Scientific Paper format. The first chapter presents an article accepted for publication in the Diversity journal, entitled "Molecular Species Delimitation Using COI Barcodes of Mealybugs (Hemiptera: Pseudococcidae) from Coffee Plants in Espírito Santo, Brazil". The dataset containing mitochondrial COI gene sequences of 26 putative of species Pseudococcidae recorded in Brazil were explored using the ASAP, GMYC, mPTP delimitation methods. An incongruence between the methods was observed, with the number of species ranging from 22 to 30 putative species. Of these, 10 species were identified among the new specimens collected in Brazil. In the second chapter, a paper to be submitted in a Journal with Impact Factor (≥ 3) is presented, entitled "Development of a High-Resolution Melting method based on COI minibarcodes to identify mealybug (Hemiptera: Pseudococcidae) pest species". A 76 bp primer pair was designed based on COI gene sequence alignment for 5 species of mealybugs. Analysis was performed by PCR immediately followed by HRM and the 5 species tested were discriminated, including two closely related species, Planococcus citri and Pl. minor. In addition, intraspecific variation was also detected in Pl. citri. The third chapter presents a paper for submission to a Journal with an Impact Factor (≥ 3), entitled "Potential distribution of the citrus mealybug, Planococcus citri (Risso, 1813), an important pest from coffee tree in Brazil”. The most abundant species found in coffee trees in Espirito Santo was chosen as a model for potential distribution prediction. Species distribution modelling was performed based on MaxEnt. North and northwest of the state was revealed to have the highest potential occurrence of this pest. This assumption was confirmed with our collection data, presented in the first chapter. This thesis presented important results on the diversity of species of mealybugs occurring in ES and another brazilian states. The tools used will help in the rapid identification of these insects and can be implemented in strategies for prophylaxis, monitoring and control of quarantine pests of agricultural crops in Brazil and other countries.
Activity of prokaryotic communities from offshore oil reservoir and their adaptation to high hydrostatic pressure
(Universidade Federal do Espírito Santo, 2023-02-15) Santos, Luiza Favarato; Fernandes, Patricia Machado Bueno; https://orcid.org/0000000326953638; http://lattes.cnpq.br/2441925791593067; https://orcid.org/0000000173409358; http://lattes.cnpq.br/1217579607633423; Fernandes, Antonio Alberto Ribeiro; https://orcid.org/0000-0003-0535-9349; http://lattes.cnpq.br/4696507759154477; Santos, Alexandre Martins Costa; https://orcid.org/0000000288018875; http://lattes.cnpq.br/4144105396879016; Rodrigues, Silas Pessini; https://orcid.org/0000-0002-8672-3626; http://lattes.cnpq.br/9067991933812274; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; Biasi, Ronaldo Sergio de
Deep-sea microbes comprise a significant portion of Earth’s biomass, but they are still poorly studied. Although the subseafloor is an oligotrophic, anoxic, dark, and pressurized environment, life still thrives in such kind of environment. In this work, three samples collected 100 m, 3,060 m, and 6,000 m deep in the Brazilian coast were investigated by analysis of 16S rRNA. A difference was found in the biological diversity between these communities at the genus level. The three communities had acid-producing bacteria and sulfate-reducing bacteria, but only the community from 3,060 m deep had a methanogenic archaeon. From the 6,000 m deep sample was isolated a bacterium of the Halanaerobium genus. Two important variables for the environment and offshore industries, bacterial survival, and hydrogen sulfide (H2S) production under high hydrostatic pressure (HHP) were investigated. Cell survival and H2S production were affected by HHP. In the case of the 3,060 m bacteria, population and H2S production increase with increasing pressure, while for the 100 m bacteria, population is stable with increasing pressure but H2S production decreases. The results show the importance of studying the influence of pressure on bacterial growth and H2S production to define strategies to mitigate economic losses arising from activities in marine environments.
Análise da composição química e da atividade antibacteriana e antifúngica de clones de Schinus terebinthifolia Raddi
(Universidade Federal do Espírito Santo, 2023-03-01) Moraes, Rodrigo; Ventura, Jose Aires; https://orcid.org/0000-0003-1422-1739 ; http://lattes.cnpq.br/8687116881326074 ; https://orcid.org/0000-0001-9175-4825; http://lattes.cnpq.br/0598172220327707; Santos, Alexandre Martins Costa; https://orcid.org/0000-0002-8801-8875; http://lattes.cnpq.br/4144105396879016; Endringer, Denise Coutinho; https://orcid.org/0000-0001-9396-2097; http://lattes.cnpq.br/6424432214565576; Kuster, Ricardo Machado; https://orcid.org/0000-0002-8961-5348; http://lattes.cnpq.br/4149814906786366; França, Hildegardo Seibert ; https://orcid.org/0000-0001-6129-8793; http://lattes.cnpq.br/1284874997224988
Aroeira (Schinus terebinthifolia Raddi) is included in the Brazilian List of Essential Medicines due to its potential to generate bioactive compounds, including those with antimicrobial properties. The occurrence of antimicrobial resistance increases health care costs, length of stay in hospitals, morbidity, and mortality. WHO has published a list of antibiotic-resistant “priority pathogens” to guide and promote research, development, and innovation of new antibiotics including those that may combat oxacillin-resistant Staphylococcus aureus and carbapenemase-producing Klebsiella pneumoniae. Fungi of the genus Fusarium are important pathogens for plants, humans, and animals, in addition to producing mycotoxins in food, with a great lack of new molecules that can be used for their control. This research sought to characterize the phytochemical profile of polar and non-polar extracts of mature fruits of four Aroeira clones and their antimicrobial activity against oxaxilin-resistant Staphylococcus aureus, carbapenemase-producing Klebsiella pneumoniae and the fungus Fusarium guttiforme. The extracts of all aroeira clones showed antimicrobial activity for bacteria and fungus, varying according to the clone and solvent used. Polar extracts showed greater antifungal and antibacterial activity, being more active on Gram-positive bacteria. The antifungal and antibacterial activity varied according to the evaluated clone. Clone BAAr 5 showed greater antibacterial activity while clones BAAr 5 and BAAr 8 had greater antifungal activity. The Aroeira clones showed similarity in the chromatographic profile by HPLC. It was possible to observe a greater relative number of peaks in clone BAAr 5 when extracted using a hydroethanolic solvent. The chromatogram of the hexane extracts showed a greater diversity of peaks. There was a greater abundance of sesquiterpenes compared to monoterpenes when the samples were evaluated in GC-MS. Spectrometry using ESI(-)FT-ICR MS identified the presence of 29 ions, between m/z 195 and m/z 925 in the polar extracts of the clones. Twenty-four ions were observed in clone BAAr 5, 20 in clone BAAr 8, 23 in clone BAAr9 and 17 in clone BAAr 23. Although the clones were grown under the same edaphoclimatic conditions, they showed variation in antimicrobial activity and bioactive compounds identified. It is suggested that the commercial use of Aroeira should come from selected and cloned plants that present the desired characteristics
Diversidade e frequência haplotípica de X-STRS na população do Espírito Santo e sua contribuição para elucidação de casos forenses complexos
(Universidade Federal do Espírito Santo, 2023-03-03) Rodrigues, Fernanda Mariano Garcia de Souza; Louro, Iuri Drumond; https://orcid.org/0000000151609615; http://lattes.cnpq.br/3817361438227180; https://orcid.org/0000000270355005; http://lattes.cnpq.br/0479360701927218; Meira, Debora Dummer; https://orcid.org/0000-0002-6092-2459; http://lattes.cnpq.br/7199119599752978; Carvalho, Elizeu Fagundes de; https://orcid.org/0000-0003-4620-7253; http://lattes.cnpq.br/2742420738858309; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; Rave, Cintia Fridman
Genetic markers Short Tandem Repeats (STR) are the center of human genetic identification, STR markers on autosomal chromosomes and on the Y chromosome being the most used. However, in some forensic situations, such as cases of suspected incest, paternity without a maternal sample for comparison, traces with mixed DNA, the use of only these markers may not be enough to solve these cases. The study of STRs markers of the X sex chromosome (X-STRs) significantly increases the probability of identification by complementing the data obtained for autosomal and Y chromosome markers. Statistical analyzes that must be included in the issuance of expert reports. Thus, the general objective of this work is to carry out a macro-regional survey of the haplotype frequencies of 12 loci of X-STRs in Espírito Santo, to estimate the genetic diversity, the dynamics of populations and promote the gain of statistical power and the updating of this information in databases international data. In this sense, the survey of haplotype frequencies of X-STRs was carried out with a sample group of 571 unrelated individuals born in the 4 macro-regions of the state, in order to obtain a reliable estimate of the genetic diversity of ES. Analyzing the set of 12 XSTRs, no statistically significant differences were found between the macro regions of the state. The allele and haplotype frequencies found here have high rates of allelic and haplotype variability, demonstrating that this X-STR set is very informative in terms of individual discrimination, and may contribute to building knowledge and the importance of using the X chromosome in routine of laboratories that use DNA technology in human identification.
Avaliação da expressão de microRNAs e proteínas como biomarcadores de diagnóstico em carcinoma epidermoide de língua
(Universidade Federal do Espírito Santo, 2023-11-22) Có, Anna Clara Gregório; Camillo, Cláudia Malheiros Coutinho; https://orcid.org/0000-0001-9016-2668; http://lattes.cnpq.br/3184503163639480; Zeidler, Sandra Lúcia Ventorin von; https://orcid.org/0000-0002-8897-5747; http://lattes.cnpq.br/5785612863130498; https://orcid.org/0000-0002-7737-0371; http://lattes.cnpq.br/3678557620411441; Nunes, Fabio Daumas; https://orcid.org/0000-0002-7785-6785; http://lattes.cnpq.br/4909755821591847; Errera, Flavia Imbroisi Valle; https://orcid.org/0000-0002-8069-6372; http://lattes.cnpq.br/9337327437538048; Arantes, Lidia Maria Rebolho Batista; https://orcid.org/0000-0001-8230-1218; http://lattes.cnpq.br/2019308149950531; Paula, Flavia de; https://orcid.org/0000-0001-8679-2982; http://lattes.cnpq.br/7913201450663683
Oral squamous cell carcinoma (OSCC) is the 16th most commonly diagnosed form of cancer globally, with a higher prevalence in the tongue compared to other areas of the oral cavity. However, the lack of effective biomarkers for diagnosis, especially for precancerous lesions, poses a limitation, as visual or histological examination cannot predict the progression of dysplastic lesions, making it difficult to determine whether they will develop into cancer or return to normal epithelium. In this context, the present research aims to investigate molecular targets that may indicate the irreversible transformation of these cells, to provide a basis for broader studies aimed at using these targets as biomarkers for early OSCC diagnosis. To achieve this goal, this experimental study addressed the evaluation of the expression of a panel of microRNAs and proteins in tumour tissues and adjacent tumour-adjacent epithelium obtained from patients with tongue squamous cell carcinoma and oral epithelium from healthy individuals. Additionally, the research explored the association between these biomarkers, seeking to determine their potential application as diagnostic biomarkers for tongue squamous cell carcinoma. A total of 75 cases of tongue squamous cell carcinoma and adjacent tumouradjacent epithelium were included in the study, and the expression of the proteins survivin, Bcl-2, PLK1, p16, p40, p63, EGFR, and cyclin D1 was analysed by immunohistochemistry. The analysis of the microRNA panel's expression involved 31 samples of tongue squamous cell carcinoma, 10 samples of healthy gingival tissue, and 10 samples of serum from healthy individuals, as well as 7 samples of serum from patients diagnosed with OSCC, using the RT-qPCR technique. In silico analysis by bioinformatics validated the findings related to the expression of differentially expressed microRNAs in the sample group. The results showed differences in the expression of miRNA-31-5p (p<0.001) and miRNA-21-5p (p=0.001) in tumour samples compared to control samples. Significant differences were not observed in the expression of miRNA-24-3p, while miRNA-542-3p and 196a-5p were not detected in the sample group. No significant difference was observed in the expression of miRNAs in serum samples. The assessment of the diagnostic potential of microRNAs included ROC curve analysis, which revealed that miR-21-5p had an area under the curve (AUC) of 0.803, while miR-31-5p obtained an AUC of 0.777. The results also identified differential expression among the proteins survivin, PLK1, and p63, all of which showed increased expression in tumour tissue. Additionally, a correlation was observed between the expression of miR-21-5p and the protein p40 (chi-square: p=0.047; Spearman correlation: r=0.402; p=0.023). In conclusion, the results suggest that miR-21-5p and miR-31-5p may be potential diagnostic biomarkers for tongue squamous cell carcinoma, providing a foundation for further exploration for large-scale studies to explore miRNA-protein correlations, considering the site specificity of miRNAs.
Caracterização físico-química de hidrolases de proteoforma de tripsina bovina e lipases de resíduos pesqueiros
(Universidade Federal do Espírito Santo, 2023-12-18) Cruz, Fabiano Torres; Santos, Alexandre Martins Costa; https://orcid.org/0000000288018875; http://lattes.cnpq.br/4144105396879016; https://orcid.org/0000-0003-2510-5354; http://lattes.cnpq.br/2548521743413029; Teixeira, Kadima Nayara; Oliveira, Jairo Pinto de; https://orcid.org/0000000175951183; http://lattes.cnpq.br/2228283301316218; Koblitz, Maria Gabriela Bello; Cicilini, Maria Aparecida
Hydrolases have significant biotechnological importance, with lipases and proteases being highlighted as essential catalysts in industrial biotechnological processes, playing fundamental roles in the production of food, beverages, biofuels, and pharmaceuticals, with positive impacts both economically and environmentally. Integrating two chapters dedicated to the detailed study of trypsin and lipase, the research aims to deepen the understanding of their properties and contributions to the evolution of biotechnology. Notable advances in enzyme research have revealed a new proteoform of bovine trypsin, showing significant differences in activity, kinetic properties, and conformational stability compared to other known forms, opening new perspectives for understanding physiological pathways of enzyme inactivation. In the context of lipases, the study focuses on the economic and environmental potential of lipases derived from tilapia viscera, exploring purification, enzyme shelf life, and economic and environmental improvements. The results indicate that lipases from tilapia viscera can be purified and become viable alternatives with economic and environmental potential for Brazil.
Potencial prognóstico de células inflamatórias e PD-L1 solúvel em carcinoma epidermoide de cabeça e pescoço
(Universidade Federal do Espírito Santo, 2024-04-16) Daniel, Camila Batista; Co-orientador1; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador2; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador3; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador4; ID do co-orientador4; Lattes do co-orientador4; Zeidler, Sandra Lúcia Ventorin von ; https://orcid.org/0000-0002-8897-5747; http://lattes.cnpq.br/5785612863130498; Orientador2; https://orcid.org/; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; Santos, Eldamária de Vargas Wolfgramm dos ; https://orcid.org/; http://lattes.cnpq.br/; Errera, Flavia Imbroisi Valle ; https://orcid.org/; http://lattes.cnpq.br/; Arantes, Lidia Maria Rebolho Batista ; https://orcid.org/; http://lattes.cnpq.br/; Mendonça, Elismauro Francisco de ; http://lattes.cnpq.br/; 5º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 6º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 7º membro da banca; https://orcid.org/; http://lattes.cnpq.br/
Immunosuppression is recognized as a hallmark of cancer and has been associated with worse outcomes. In head and neck squamous cell carcinoma (HNSCC), immunosuppression is a hallmark of the tumour and may be mediated by immunosuppressive inflammatory cells and PD-L1 expression. Given their participation in this process, our study aimed to describe the prognostic impact of these elements in tumour tissue and peripheral blood HNSCC patients. We conducted a multicentre retrospective study with biological samples and clinical data from HNSCC patients recruited at two centres in Brazil and the United Kingdom and healthy individuals. To analyse the inflammatory infiltrate, we used tumour tissue slides stained with haematoxylin and eosin and immunohistochemistry for PD-L1 analysis and neutrophil quantification. Absolute leukocyte counts were retrieved from pretreatment blood counts available in medical records. To evaluate the prognostic value of PD-L1 in liquid biopsy, we used serum and plasma samples obtained from patients and healthy individuals for quantification of soluble PD-L1 (sPD-L1) by ELISA. Fresh tumour tissue samples were used to analyse CD274 gene expression levels using the RT-qPCR technique. Furthermore, we analysed PD-L1 expression by flow cytometry in HNSCC cell lines and quantified sPD-L1 levels in the supernatant. The tumour microenvironment analysis showed that low levels of lymphocytes are associated with worse overall survival and disease-free survival. However, we did not observe this relationship with tumour-associated neutrophils and tumour PD-L1. When analysed together in a scoring system, we demonstrated that low levels of lymphocytes, high expression of PD-L1 and high infiltration of neutrophils are associated with a worse outcome. In blood, a high ratio of neutrophils and lymphocytes (NLR) was also associated with worse survival but was not correlated with inflammatory components of the tumour. High levels of sPD-L1 protein were associated with reduced overall survival, however our study did not identify a relationship between sPD-L1 levels in the blood and PD-L1 expression in tumour tissue, determined by immunohistochemistry and RT-qPCR. In contrast, the in vitro study showed that the levels of sPD-L1 released 14 into the supernatant are strongly correlated with cytoplasmic and membrane expression. Our data further showed that sPD-L1 levels in patients were positively correlated with the number of leukocytes, neutrophils and monocytes in peripheral blood. Taken together, our results highlight the prognostic potential of markers of the tumour microenvironment analysed in a combined manner, in a scoring system, as a way of providing a more comprehensive overview of tumour behaviour, highlighting important events, such as immunosuppression. Our results highlight the prognostic potential of PD-L1 detected by liquid biopsy in HNSCC and indicate that the levels detected in the blood do not necessarily correspond to what is observed in the tumour. Therefore, it is believed that it should be analysed as an independent marker whose immunosuppressive role is carried out at a systemic level
Avaliação do potencial bioativo de frações peptídicas de coprodutos de peroá (balistes capriscus)
(Universidade Federal do Espírito Santo, 2024-04-30) Ribeiro, Monique Lopes; Santos, Alexandre Martins Costa; https://orcid.org/0000-0002-8801-8875; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; Cicilini, Maria Aparecida; https://orcid.org/; http://lattes.cnpq.br/; Oliveira, Jairo Pinto de; https://orcid.org/; http://lattes.cnpq.br/; Koblitz, Maria Gabriela Bello; https://orcid.org/; http://lattes.cnpq.br/; Souza, Bartolomeu Warlene Silva de; http://lattes.cnpq.br/
Fish industry generates a significant amount of waste which have high biological value and potential for industrial use. The aim of this study was to evaluate the antioxidant and ACE-inhibitory activities of peptide fractions from triggerfish (Balistes capriscus) processing coproducts. Molecular mass distribution of the proteins extracted from fish viscera was determined by molecular exclusion chromatography presenting fractions with a wide range of molecular mass (<1.2 kDa to > 440 kDa). Soluble Protein Extract (SPE) was hydrolysate using papain (HP), bromelain (HB) and trypsin (HT) (3% p.p-1, 6h). Samples were fractionated (>100 MWCO, 30–100, 10-30 and < 10 MWCO). Antioxidant activity of fractions was evaluated and fraction SPE4 (<10 MWCO) showed the highest value of Trolox Equivalent Antioxidant Capacity - TEAC (10,157.7 μmol Trolox. g-1) and Ferric Reducing Antioxidant Power - FRAP (1,588.71 μmol FeSO4. g-1 ). SPE and hydrolysates (<10MWCO) were separated by ion-exchange chromatography. Fraction F1 showed the highest value for TEAC capacity (8,839.04 μmol Trolox. g-1) and FRAP (1,749.94 μmol FeSO4. g-1). ACE-inhibitory activity was evaluated for non-hydrolysate and hydrolysate fractions. Fractions F3, F5 and HP3 showed the lowest IC50 value (30.1, 42.7 e 37.7 µg, respectively). Antimicrobial activity was observed in samples SPE (against S. aureus), F1 and F4 (against Pseudomonas sp.) It was identified 20 amino acid sequences that could contribute to the biological activity of the peptide fractions. Proteins extracted from triggerfish viscera demonstrated to be a good source of bioactive peptides that may have food and pharmaceutical applications.

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