Biotecnologia

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Programa de Pós-Graduação em Biotecnologia

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URL do programa: http://www.biotecnologia.ufes.br/pt-br/pos-graduacao/PPGBIOTEC

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Activity of prokaryotic communities from offshore oil reservoir and their adaptation to high hydrostatic pressure
(Universidade Federal do Espírito Santo, 2023-02-15) Santos, Luiza Favarato; Fernandes, Patricia Machado Bueno; https://orcid.org/0000000326953638; http://lattes.cnpq.br/2441925791593067; https://orcid.org/0000000173409358; http://lattes.cnpq.br/1217579607633423; Fernandes, Antonio Alberto Ribeiro; https://orcid.org/0000-0003-0535-9349; http://lattes.cnpq.br/4696507759154477; Santos, Alexandre Martins Costa; https://orcid.org/0000000288018875; http://lattes.cnpq.br/4144105396879016; Rodrigues, Silas Pessini; https://orcid.org/0000-0002-8672-3626; http://lattes.cnpq.br/9067991933812274; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; Biasi, Ronaldo Sergio de
Deep-sea microbes comprise a significant portion of Earth’s biomass, but they are still poorly studied. Although the subseafloor is an oligotrophic, anoxic, dark, and pressurized environment, life still thrives in such kind of environment. In this work, three samples collected 100 m, 3,060 m, and 6,000 m deep in the Brazilian coast were investigated by analysis of 16S rRNA. A difference was found in the biological diversity between these communities at the genus level. The three communities had acid-producing bacteria and sulfate-reducing bacteria, but only the community from 3,060 m deep had a methanogenic archaeon. From the 6,000 m deep sample was isolated a bacterium of the Halanaerobium genus. Two important variables for the environment and offshore industries, bacterial survival, and hydrogen sulfide (H2S) production under high hydrostatic pressure (HHP) were investigated. Cell survival and H2S production were affected by HHP. In the case of the 3,060 m bacteria, population and H2S production increase with increasing pressure, while for the 100 m bacteria, population is stable with increasing pressure but H2S production decreases. The results show the importance of studying the influence of pressure on bacterial growth and H2S production to define strategies to mitigate economic losses arising from activities in marine environments.
Alta pressão hidrostática como ferramenta de seleção direcionada em S. cerevisiae para produção de etanol 2g pela casca de coco verde
(Universidade Federal do Espírito Santo, 2019-03-11) Santos, Luiza Favarato; Fernandes, Patricia Machado Bueno; Santos, Alexandre Martins Costa; Torres, Fernando Araripe Gonçalves
The green coconut shell is a pollutant residue of the environment generated to tons in Brazil and that brings several environmental problems in its disposal. Therefore, using it for the production of renewable energy is an important sustainable solution for the mitigation of the environmental impact and for the generation of energy. Yeast Saccharomyces cerevisiae represents one of the microorganisms most used in the fermentation industries for the production of biofuels from lignocellulolytic residues. There are already on the market strains of this yeast capable of degrading lignocellulolytic residues, which have been developed to increase the yield of the process, making it feasible financially on an industrial scale. The high pressure hydrostatic (HHP) is a tool that simulates three types of different stresses in the yeast: the ethyl, the thermal and the oxidative, which are very common in fermentation dornas. In addition, studies have shown that fermentation occurs more rapidly when the yeast is under HHP. Therefore, HHP can be used as a targeted selection tool to obtain a strain with higher fermentation capacity and greater resistance to fermentation stresses, increasing the yield of the process. The aim of this work was to apply a selective pressure to the S. cerevisiae strain G2-104 using high hydrostatic pressure in order to obtain a strain with a higher fermentation capacity in green coconut shell culture medium, to increase the yield of the second generation ethanol production process.
Alterações na atividade fotossintética em folhas do mamoeiro ‘Golden’ infectadas com PMeV, detectadas por meio da fluorescência da clorofila a
(Universidade Federal do Espírito Santo, 2017-07-13) Hernández, Karla Verónica Alvarado; Silva, Diolina Moura; Moura, Andrea Bittencourt; Ventura, José Aires; Falqueto, Antelmo Ralph
Papaya (Carica papaya l.) is one of the most consumed fruits in the world due to its nutritional, pharmaceutical and use in the food and cosmetic industry. In Brazil, the second producer of papaya in the world, the production has been severely affected by meleira, disease which is caused by the complex of Papaya meleira virus (PMeV and PMeV2). In order to analyze the changes in photosynthetic activity in 'Golden' papaya leaves infected and not infected by PMeV, the experiment was carried out in which three groups of plants were evaluated: Control, without inoculation; Inoculated with sodium phosphate buffer (Treatment = ↓ [𝑃𝑀𝑒𝑉2]) and inoculated with sodium phosphate buffer and latex harvested from plants with meleira symptoms (Treatment = ↑[PMeV2]). The chlorophyll a fluorescence technique was used as a tool. There was a significant increase in quantum photochemical energy conversion in photosystem II (Y (II)) and an increase in the quantum yield of the regulated non-photochemical energy loss (Y (NPQ)) in the inoculated plants. At the same time, transient fluorescence curves showed greater efficiency in the reduction of the primary (Quinone A) and secondary (Quinone B) acceptors of electrons in photosystem II and greater efficiency of reduction of the plastoquinone pool and, consequently, greater efficiency in the reduction of acceptors of the Citb6f complex. The presence of two PMeV viruses and PMeV2 confirmed the presence of two PMeV viruses. A higher photochemical performance index (PItotal) was observed in the plants with the highest viral load from the 35th day after inoculation (DAI), and at 49 DAI, an increase in the reduction activity of photosystem I and PItotal was observed in plants with higher viral load and intermediate values in plants with lower viral load. These results suggest a defensive response of papaya cv. Golden to infection and confirm the presence of the two viruses, PMeV and PMeV2. Therefore, this technique proved to be a tool with great potential as a simple and fast method to study the interaction plant x pathogen and as the mechanisms of virus infection in plants and could also be used to detect the changes in photosynthetic activity in papaya infected with PMeV and PMeV2.
Alterações na parede celular e no metabolismo energético de Saccharomyces cerevisiae submetida à alta pressão hidrostática
(Universidade Federal do Espírito Santo, 2018-02-20) Carneiro, Tárcio; Fernandes, Patrícia Machado Bueno; Fernandes, Antonio Alberto Ribeiro; Santos, Alexandre Martins Costa; Zingali, Russolina Benedeta
Yeasts are one of the most important organisms in the processes of industrial biotechnology. Its great fermentative capacity and considered as a genetically safe organism, made this organism one of the first to be domesticated by the human being. Among its applications are not only its products generated from the fermentation, but also its cell itself, which moves a market ranging from organic yeast to yeast paste for human consumption. Understanding not only the fermentative process, but also the respiratory process are necessary. The production processes in which the yeasts are employed expose them to abiotic stresses of different types, such as temperature variation, pH, salinity and others. High hydrostatic pressure is now an important methodology in the study of its effects on the cells of Saccharomyces cerevisiae, acting as a model of stress. The understanding of this phenomenon on cells has helped not only to understand the processes of response to stress, but also its use as an important tool in industrial application. Atomic force microscopy, high performance liquid chromatography (HPLC) and microarray data were used to characterize the effects of high hydrostatic pressure on the cell wall of Saccharomyces cerevisiae and its energy metabolism. For the production of the experiments, BT0510 cells were submitted to high hydrostatic pressure (50 to 200 MPa) for 30 minutes and then analyzed by atomic force microscopy and in another experiment the cells were subjected to a pressure of 50MPa for 30 minutes and facing to agitation. The results showed the appearance of resistance bands in the cell wall at pressures of 100, 150 and 200 MPa, indicating possible fracture points, which leads to lesions that compromise its viability. At pressures of 50MPa the variation of the resistance of the cell wall resembles the control, which shows that this pressure is of sublethal character, not interfering so expressively in the cellular mortality. The response to 50 MPa treatment was then analyzed in the long term by HPLC, with collection points at 1, 2, 3, 4, 12, 36 and 60 hours after application of pressure. The results show high affinity with microarray generated data, where the cells increase the rate of glucose consumption in the fermentative phase (1 to 12 hours) as well as higher ethanol production. The cells also showed a greater efficiency in the ethanol metabolism which generated an increase in the cell mass in the cells treated with pressure. It was demonstrated that high hydrostatic 6 pressure has industrial application in cellular mass acquisition processes as in the production of biological yeast, thus showing the biotechnological capacity of the use of this stress mechanism and generating the deposit of the patent "Process for the increase of cellular mass in yeasts using high hydrostatic pressures ".
Análise da composição química e da atividade antibacteriana e antifúngica de clones de Schinus terebinthifolia Raddi
(Universidade Federal do Espírito Santo, 2023-03-01) Moraes, Rodrigo; Ventura, Jose Aires; https://orcid.org/0000-0003-1422-1739 ; http://lattes.cnpq.br/8687116881326074 ; https://orcid.org/0000-0001-9175-4825; http://lattes.cnpq.br/0598172220327707; Santos, Alexandre Martins Costa; https://orcid.org/0000-0002-8801-8875; http://lattes.cnpq.br/4144105396879016; Endringer, Denise Coutinho; https://orcid.org/0000-0001-9396-2097; http://lattes.cnpq.br/6424432214565576; Kuster, Ricardo Machado; https://orcid.org/0000-0002-8961-5348; http://lattes.cnpq.br/4149814906786366; França, Hildegardo Seibert ; https://orcid.org/0000-0001-6129-8793; http://lattes.cnpq.br/1284874997224988
Aroeira (Schinus terebinthifolia Raddi) is included in the Brazilian List of Essential Medicines due to its potential to generate bioactive compounds, including those with antimicrobial properties. The occurrence of antimicrobial resistance increases health care costs, length of stay in hospitals, morbidity, and mortality. WHO has published a list of antibiotic-resistant “priority pathogens” to guide and promote research, development, and innovation of new antibiotics including those that may combat oxacillin-resistant Staphylococcus aureus and carbapenemase-producing Klebsiella pneumoniae. Fungi of the genus Fusarium are important pathogens for plants, humans, and animals, in addition to producing mycotoxins in food, with a great lack of new molecules that can be used for their control. This research sought to characterize the phytochemical profile of polar and non-polar extracts of mature fruits of four Aroeira clones and their antimicrobial activity against oxaxilin-resistant Staphylococcus aureus, carbapenemase-producing Klebsiella pneumoniae and the fungus Fusarium guttiforme. The extracts of all aroeira clones showed antimicrobial activity for bacteria and fungus, varying according to the clone and solvent used. Polar extracts showed greater antifungal and antibacterial activity, being more active on Gram-positive bacteria. The antifungal and antibacterial activity varied according to the evaluated clone. Clone BAAr 5 showed greater antibacterial activity while clones BAAr 5 and BAAr 8 had greater antifungal activity. The Aroeira clones showed similarity in the chromatographic profile by HPLC. It was possible to observe a greater relative number of peaks in clone BAAr 5 when extracted using a hydroethanolic solvent. The chromatogram of the hexane extracts showed a greater diversity of peaks. There was a greater abundance of sesquiterpenes compared to monoterpenes when the samples were evaluated in GC-MS. Spectrometry using ESI(-)FT-ICR MS identified the presence of 29 ions, between m/z 195 and m/z 925 in the polar extracts of the clones. Twenty-four ions were observed in clone BAAr 5, 20 in clone BAAr 8, 23 in clone BAAr9 and 17 in clone BAAr 23. Although the clones were grown under the same edaphoclimatic conditions, they showed variation in antimicrobial activity and bioactive compounds identified. It is suggested that the commercial use of Aroeira should come from selected and cloned plants that present the desired characteristics
Análise da excitabilidade e conectividade cortical para um sistema de neurorreabilitação baseado em interface-cérebro máquina e monociclo robótico
(Universidade Federal do Espírito Santo, 2021-06-29) Cardoso, Vivianne Flávia; Bastos Filho, Teodiano Freire; https://orcid.org/0000000211852773; http://lattes.cnpq.br/3761585497791105; https://orcid.org/0000-0002-1654-3320; http://lattes.cnpq.br/1122896995621553; Nogueira, Breno Valentim; https://orcid.org/0000000221990635; http://lattes.cnpq.br/0011229320439147; Andrade, Adriano de Oliveira; https://orcid.org/0000-0002-5689-6606; http://lattes.cnpq.br/1229329519982110; Caldeira, Eliete Maria de Oliveira; https://orcid.org/0000-0002-3742-0952; http://lattes.cnpq.br/8508979665054143; Frizera Neto, Anselmo; https://orcid.org/0000000206873967; http://lattes.cnpq.br/8928890008799265; Rodriguez, Denis Delisle; https://orcid.org/000000028937031X; http://lattes.cnpq.br/7140331839822423
Recently, studies on cycling-based brain-computer interfaces (BCIs) have been standing out due to their potential for lower-limb recovery. In this scenario, the behavior of the sensory motor rhythms and the brain connectivity present themselves as source of information that can contribute to interpret the cortical effect of these technologies. This study aims to analyze how motor sensory rhythms and the cortical connectivity behave when volunteers command a reactive motor imagery (MI) BCI that provides passive pedaling feedback. Eight healthy subjects performed pedaling MI to command an Electroencephalography (EEG) based BCI with motorized pedal to receive passive movements as feedback. The EEG data were analyzed under the following four conditions: resting, MI calibration, MI online and receiving passive pedaling (online phase). Most subjects produced around Cz, corresponding to the foot area significant event-related desynchronization (ERD) patterns around Cz, when performing MI and receiving passive pedaling. The sharpest decrease was found for low β band (13 – 22 Hz). The connectivity results revealed an exchange of information between supplementary motor area (SMA) and parietal regions during MI and passive pedaling. Findings point the primary motor cortex activation for most participants and the connectivity between SMA and parietal regions during pedaling MI and passive pedaling.
Análise da expressão dos genes relacionados à parede celular e a membrana plasmática de Saccharomyces cerevisiae em resposta a alta pressão hidrostática
(Universidade Federal do Espírito Santo, 2012-02-28) Souza, Diego Trindade de; Fernandes, Patrícia Machado Bueno; Masuda, Cláudio Akio; Guimarães, Marco César Cunegundes; Bravim, Fernanda
The yeast Saccharomyces cerevisiaehas received much attention for its applications in the biotechnology industry. However, during these processes, yeasts are exposed to many different types of stress. Knowing the mechanisms S. cerevisiaeuses to respond to these stresses is essential for obtaining higher yields in the industrial sector. High hydrostatic pressure (HHP) is a stress that exerts a broad effect on S. cerevisiae, interfering with cell membranes, cellular architecture, and various biochemical processes. In this study, we used the microarray technique to investigate the influence of HHP on genes related to the cell wall and plasma membrane. For this, cells from BT0605 strain were subjected to hydrostatic pressure of 50 MPa for 30 min, and then incubated at ambient pressure for 5, 10 and 15 min.The microarray results clearly demonstrated that of the genes involved in biogenesis of the cell wall, those which are related to protection were readily activated by high pressure, whilst the genes involved in cell wall structure required 5 minutes on the ambient pressure to be induced.
Análise da expressão temporal de genes relacionados ao metabolismo de Saccharomyces cerevisiae em resposta ao estresse por alta pressão hidrostática
(Universidade Federal do Espírito Santo, 2012-02-29) Silva, Lucas Ferreira da; Fernandes, Patrícia Machado Bueno; Masuda, Cláudio Akio; Louro, Iuri Drummond
Living organisms maintain a complex internal balance, which enables them to optimise their growth and metabolism. However, fluctuations in environmental conditions can affect this balance. The yeast Saccharomyces cerevisiae is a unicellular, eukaryote model organism. The biotechnological processes it is used for include the production of bread, cheese, alcohol, drugs and pharmaceuticals. From a physiological standpoint, all these processes affect the normal operation of the cell and it is extremely important to study the molecular mechanisms that provide protection against these stressful situations. One of the models used to study stress is high hydrostatic pressure (HHP), which is known to cause morphological and metabolic changes in yeast. Several studies have demonstrated that the expression pattern of genes from yeast submitted to high hydrostatic pressure shows a complex profile, including genes related to oxidative, osmotic, and heat protection.
Análise da fibrose do tecido adiposo de mulheres submetidas à abdominoplastia e cirurgia bariátrica
(Universidade Federal do Espírito Santo, 2014-10-28) Castro, Eduardo José Passamai de; Errera, Flávia Imbroisi Valle; Nogueira, Breno Valentim; Nahas, Fábio Xefran
Fibrosis is an exceeding accumulation of extracellular matrix, resulting from an unbalance between the synthesis and degradation of its components. It is associated with the metabolic alterations in the adipose tissue, however, its occurrence in the different deposits and clinical repercussions are not yet fully understood. The study aims at assessing fibrosis in the adipose tissue regarding the presence of obesity, location of the deposit [subcutaneous abdominal adipose tissue (SAAT) and the visceral one (VAT)] and its association with clinical variables. Samples of fat from SAAT and VAT were obtained from 21 women undergoing bariatric surgery (IMC>40kg/m²) and 25 VAT samples from the ones undergoing abdominoplasty (IMV<30kg/m²). The samples were processed for conventional histology. The red stain picrosirius was used to evaluate the total collagen fibers. The pictures obtained were analyzed in ADIPOSOFT®. The fibrosis percentage found in SAAT and VAT were analyzed through non-parametric static tests, using the value of p<0,05. SAAT amount present was bigger in women with obesity (p<0.0006). The fibroses between the deposits of SAAT and VAT was only observed among mulatto and black women with obesity (p<0,012). The amount of fibrosis in SAAT was not correlated to the variable clinics in women without obesity. However, with the ones undergoing bariatric surgery, it was observed correlations of fibrosis in SAAT with body mass index (BMI), glycated hemoglobin (A1c), LDL, triglycerides; and in VAT with the percentage of preoperative fat loss, percentage of total fat loss, percentage of pre lean mass, Basal Metabolic Rate (BMR) and Basal Energetic Consumption (BEC). The metabolic parameters and the anthropometric profile of the bariatric surgery were associated to the fibrosis in SAAT, while the post-surgery parameters were associated to the fibrosis in VAT.
Análise da influência de polimorfismos do gene CYP1B1 materno na idade gestacional de nascimento : uma correlação clínica e molecular
(Universidade Federal do Espírito Santo, 2017-04-20) Santos, Jéssica Aflávio dos; Louro, Iúri Drumond; Carvalho, Elizeu Fagundes de; Santos, Marcelo dos
Currently, both prematurity and births occurring in the late preterm and in the early term have been highlighted by their negative influence on infant mortality and morbidity. Several risk factors have been associated with decreased gestational age at birth. Environmental risk factors include cigarette smoking, excessive alcohol intake, ethnicity, weight and maternal age. Regarding genetic factors, genetic polymorphisms involved in oxidative stress and xenobiotics metabolism have been strongly associated with preterm birth. This may generate oxidative stress, considered a primary etiological factor for preterm birth because it can cause a compromised placenta. This is the case of the CYP1B1 gene, which also plays an important role in the estrogen synthesis, one of the most important hormones in pregnancy maintenance. The action of the enzyme CYP1B1 may result in the production of reactive and carcinogenic compounds due to the preferential hydroxylation of 17β-estradiol at the 4-hydroxy position. The polymorphisms rs10012, rs1056827, rs1056836 were selected for this work because they are associated with variations in this enzyme activity. Thus, the present study aims to investigate the possible influence of these polymorphisms, as well as clinical data and maternal life habits in gestational age at birth. For such, a prospective cohort study was carried out with pregnant women from the Santo Antônio de Jesus city, in State of Bahia, Brazil. Mothers were followed during gestation, delivery and puerperium between 2009 and 2016. Maternal characteristics and habits were obtained through standardized questionnaires. Genotyping was performed from blood samples from pregnant women with TaqMan® assays using the Real Time Polymerase Chain Reaction (PCR) technique. Pregnant women who delivered in the initial full-term period had higher mean maternal age and pre-gestational Body Mass Index (BMI) than the preterm and full-term periods. Pre-gestational BMI intervals, race, smoking, and alcoholic showed no significant association with gestational age at birth. A significant correlation was detected between the SNP rs1056836 and the maternal age and the pre-gestational BMI. None of the other analyzed variables showed a significant association with any of the other polymorphisms. Non direct association was detected between any of the analyzed polymorphisms and gestational age at birth. From the results obtained by this work, a possible relationship of the CYP1B1 gene with the gestational age at birth can not be ruled out. Subsequent studies should analyze the possible association of these polymorphisms with gestational age at birth in other ethnic groups, as well as carry out more extensive investigations including other genes and polymorphisms, as well as prematurity environmental risk factors.
ANÁLISE DA MEMÓRIA EPISÓDICA E SUA RELAÇÃO COM A METILAÇÃO DO GENE NR3C1 EM ADULTOS BRASILEIROS: ASSOCIAÇÃO COM VARIÁVEIS SOCIODEMOGRAFICAS E DE ESTILO DE VIDA
(Universidade Federal do Espírito Santo, 2020-03-06) Moreno, Ivana Alece Arantes; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108; https://orcid.org/; http://lattes.cnpq.br/; Arantes, Lidia Maria Rebolho Batista; https://orcid.org/; http://lattes.cnpq.br/; Freitas, Flavia Vitorino; https://orcid.org/; http://lattes.cnpq.br/
The aim of this study was to assess cognitive impairment and analyze its relationship with socioeconomic variables, lifestyle, depressive symptoms, food and nutritional security and DNA methylation levels in the NR3C1 promoter region in the peripheral blo
ANÁLISE DA PRESENÇA DE DANO INESPECÍFICO MEDIADO POR CÉLULAS T CD8 NO PULMÃO DE PACIENTES COM COVID-19
(Universidade Federal do Espírito Santo, 2022-08-12) Lopes, Paola de Oliveira; Gomes, Daniel Claudio de Oliveira; https://orcid.org/; http://lattes.cnpq.br/3965075540969796; https://orcid.org/000000031062694X; http://lattes.cnpq.br/; Pereira, Fausto Edmundo Lima; https://orcid.org/; http://lattes.cnpq.br/4065537941002091; Goncalves, Ricardo; https://orcid.org/; http://lattes.cnpq.br/
abstract
Análise da relação entre depressão e hábito tabagista na metilação do gene BDNF
(Universidade Federal do Espírito Santo, 2021-07-07) Quaioto, Bárbara Risse; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108; https://orcid.org/0000-0002-3062-7619; http://lattes.cnpq.br/7948833367111238; Arantes, Lidia Maria Rebolho Batista; https://orcid.org/0000-0001-8230-1218; http://lattes.cnpq.br/2019308149950531; Hollais, André Willian; https://orcid.org/0000-0002-2991-8646; http://lattes.cnpq.br/0068789063590867
Depression is one of the most common diseases between the cause of global inability, with millions of people affected. It is a heterogeneous and complex disease, which involves several risk factors. Although there is no single theory to explain its development, studies suggest a relationship with lower levels of brain derived neurotrophic factor (BDNF). In this sense, BDNF gene methylation, influenced by environmental factors, may be involved. It is known that individuals with depression are frequently smokers and it has already been demonstrated that changes in BDNF protein levels can occur due to tobacco use. However, the investigation of tobacco effect on BDNF gene methylation has not yet been investigated. Thus, the aim of this study was to evaluate the relationship between depression, smoking and BDNF gene methylation levels. To that end, a cross-sectional study was carried out with individuals assisted by the SUS primary health care network in the municipality of Alegre – ES. Data collection was performed using a socioeconomic, health and lifestyle assessment questionnaire, as well as anthropometric and depression assessment, using the Beck Depression Inventory-II (BDI-II). In addition, blood was collected for genomic DNA extraction and posterior methylation analysis. According to the Poisson regression, the highest prevalence of depression in the sample was explained by the female gender, not having a marriage bond, lack of leisure activities, smoking and BDNF methylation. Mann-Whitney and Generalized Linear Model analysis showed that depressed patients had higher levels of methylation, as well as non-smokers. As demonstrated by the Kruskal-Wallis test, followed by Dunn's posthoc test, smoking habit reduces BDNF methylation levels in depressed individuals, but not in non-depressed individuals. These results provide support for depression prevention policies. Furthermore, the data contribute to the understanding of the pathophysiology and diagnosis molecular of depression, involving the BDNF gene. Additionally, the results expose the need to assess the influence of smoking habit in studies on epigenetic changes in depression.
Análise de custo do tratamento do câncer da cavidade oral e orofaringe: Uso do método de microcusteio por bottom-up para dados de um hospital de referência no Brasil
(Universidade Federal do Espírito Santo, 2024-03-11) Zorzaneli, Willene dos Santos Machado; Zara, Ana Laura Amâncio de Sene; Zeidler, Sandra Ventorin von; https://orcid.org/0000-0002-8897-5747; http://lattes.cnpq.br/5785612863130498; Paula, Flavia de; Guimarães, Michele Ferreira; Silva, Everton Nunes da
Cost analysis in OC/OPC is scarce, however, necessary since cost can be an essential tool for decision-making. This study aimed to estimate the direct costs of oral and oropharyngeal squamous cell carcinoma (OC/OPC) in a state in southeastern Brazil. To this end, a cost of illness study was carried out based on the bottom-up microcosting method as a partial economic assessment, considering the direct medical costs related to outpatients and inpatients from the perspective of reimbursement rates from the Unified Health System. Data were collected from the medical records of 41 randomized patients recruited, between 2013 and 2018. All cases were confirmed as squamous cell carcinoma of the mouth and oropharynx (C02 to C06, C09 and C10 according to the International Classification of Diseases for Oncology, 3rd Edition). Each cost value was adjusted for annual accumulated inflation. The statistical analysis of descriptive analyses, parametric tests, ANOVA and Student t test and non-parametric tests, Kruskal Wallis and Mann Whitney, was used using the SPSS® Statistical program. To adjust the differences in confidence intervals at 95%, the bootstrap method was used for 5.000 resamples using the Stata® statistical program. For all statistical tests, the significance level was set at 5%. Healthcare system cost estimates for 41 patients with OC/OPC amounted to a total cost of I$164,671.07 with an average of IS$4,042.5 per patient. 22,814 procedures and uses of health services were carried out. The sample was mostly made up of men (>80%) and aged between 51 and 60 years (41.5%). Carcinomas of the base of the tongue and floor of the mouth had the highest costs, I$4,288.25 and 6,586.88, respectively. Clinical stage III was the one with the most procedures and highest costs, I$5,415.11 (p<0.001) per patient. From the perspective of the Unified Health System, there is a high-cost demand for OC/OPC treatment, especially in advanced stages of the disease. This study contributes to decision- making regarding treatment as well as the implementation of programs aimed at early detection of diseases
Análise de EEG de pacientes pós-AVC: comparativo de movimento real de membro inferior e imaginação motora
(Universidade Federal do Espírito Santo, 2022-09-16) Ferreira, Matheus Modolo; Bastos Filho, Teodiano Freire; https://orcid.org/0000000211852773; http://lattes.cnpq.br/3761585497791105; Caldeira, Eliete Maria de Oliveira; Rodriguez, Denis Delisle; https://orcid.org/000000028937031X; http://lattes.cnpq.br/7140331839822423
Cerebral Vascular Accident (CVA) affects thousands of people around the world, consequently bringing motor and visual damage, speech difficulties and other damage. It is known that the voluntary movement of the limbs reflects in a cerebral activation mainly on the cerebral motor cortex that can be captured by electroencephalogram (EEG) electrodes, however, similarly, an increase in cortical activity is also noticed during Motor Imagination activities ( IM) situation where the individual just thinks about the movement without actually performing it. This research used data from two patients from the Centro de Reabilitação Física do Espírito Santo (CREFES) who suffered a stroke to participate in the MI and active pedaling tests. The data were submitted to filtering through the Fast Fourier Transform, from the English Fast Fourier Transform (FFT) and Common Average Reference, from the English Common Average Reference (CAR), after the data were analyzed by Matlab software to determine the Relative Power (PR ). The results of the PR evaluation were compared during the active pedaling and IM task for each research participant, analyzing the alpha (8 to 12 Hz), low beta (13 to 22 Hz) and high beta (23 to 30 Hz) bands. ). The similarity ratio between active pedaling and MI reached 85.6% for one of the analyses, demonstrating levels of brain activation during MI similar to active pedaling
ANÁLISE DE FATORES DE VIRULÊNCIA RELACIONADOS À ADESÃO E AO FENÓTIPO DE VARIANTE DE PEQUENAS COLÔNIAS EM CEPAS DE Staphylococcus aureus ISOLADAS DE INFECÇÃO ÓSSEA MAXILOFACIAL
(Universidade Federal do Espírito Santo, 2020-12-21) Soares, Dalliane Oliveira; Zeidler, Sandra Lucia Ventorin Von; https://orcid.org/0000000288975747; http://lattes.cnpq.br/5785612863130498; https://orcid.org/0000000217320727; http://lattes.cnpq.br/; Gomes, Daniel Claudio de Oliveira; https://orcid.org/; http://lattes.cnpq.br/3965075540969796; Santos, Andre Luis Souza dos; https://orcid.org/; http://lattes.cnpq.br/
Osteomyelitis is the main infectious disease that affects bone tissue. The most frequent etiologic agent in this type of infection is Staphylococcus aureus, which can be found in the normal microbiota of the skin and mucous membranes in humans, but in fav
Análise de ministr's em impressões digitais depositadas em suportes variados
(Universidade Federal do Espírito Santo, 2014-06-24) Vieira, Carolina Mayumi; Louro, Iúri Drumond; Paneto, Greiciane Gaburro; Rave, Cintia Fridman; Schuenck, Ricardo Pinto
The human fingerprint identification is a simple, fast and economical method. However, blurred prints deposited on rough surfaces constitute a challenge because of the loss of details. The development methods are insufficient to allow the analysis of the fingerprint, as observed points may be blurred or damaged. With the advances in DNA genotyping technology, small amounts of biological material such as a dozen cells can yield a complete DNA profile and be of great help in criminal investigations. In this context, fingerprints, previously only analyzed from the lophoscopic aspect, can now have a genetic approach. The use of MiniSTR 's combined with an increase in the number of PCR cycles can improve the detection of DNA in samples with degraded and/or limited amount of DNA. When investigating a crime scene, the forensic expert should keep in mind there is no hierarchy among evidence and every vestige should be analyzed considering its feasibility and effectiveness in generating proof.
Análise de mutações em formas recessivas de pacientes com osteogênese imperfeita do Espírito Santo : comparação de metodologias
(Universidade Federal do Espírito Santo, 2012-02-17) Quirino, Geise de Aguiar; Paula, Flavia de; Errera, Flavia Imbroisi Valle; Bortolini, Eliete Rabi
Osteogenesis Imperfecta (OI) is a genetic desease characterized by patient s bone fragility and deformity, in which severity ranges from a barely detectable connective tissue disorder to lethality in the perinatal period. The diversity of clinical variability in patients is caused by the different location or type of mutations in one of the ten genes related with the disease. This wide clinical variability difficults the perfect clinical diagnoses, due to that the use of molecular biology techniques becomes necessary to obtain a correct diagnoses and for genotype: phenotype correlation. One of the relevant genes associated with recessive forms of OI is the LEPRE-1 gene, responsible for encoding the prolyl 3 hidroxylase 1 protein. This protein and two others are components of the complex responsible for pro-collagen alfa 1 chains 3 prolyl hydroxylation. The target of this research was to analyze the LEPRE-1 gene in eight non consanguineous patients clinically diagnosed as severe Osteogenesis Imperfecta suggestive of autossomic recessive heritage by DNA sequencing of exons 1, 3, 5, 6 and 14 of the gene. In addition, the data obtained was used to analyze the efficiency of the SSCP technique by comparing the results between screening for mutations methodologies and gene sequencing methodologies. On exon 6, for instance, a mutation in one patient was found: a heterozygose base change (c.1087A>G / p.Lys363Glu), consequently, lysine was produced instead of glutamic acid. On the other exons, there was no mutation found on the patients chosen. All the results obtained in this research were compatible with datas generated by SSCP and suggest high efficient of SSCP technique for LEPRE-1 gene to recessive cases of Osteogenesis Imperfecta
Análise de um sistema de reabilitação para membros superiores utilizando ambiente de realidade virtual baseado em Kinect e sEMG
(Universidade Federal do Espírito Santo, 2016-02-29) Cardoso, Vivianne Flavia; Frizera Neto, Anselmo; Bastos Filho, Teodiano Freire; Nogueira, Breno Valentim; Ferreira, André; Andrade, Adriano de Oliveira
Impaired motor function appears as one of the most common symptoms stroke. When the upper limbs are affected, carrying out daily activities is compromised. The recent developments in the field of rehabilitation are exercises in virtual reality environment (RV). Another method used for rehabilitation of the upper limbs of patients after stroke is the EMG biofeedback. Through this biofeedback, the myoelectric signals from the muscle are converted into visual and audio information that allows the patient to control and regulate muscle activity. The objective of this work is to design and evaluate a new form of rehabilitation by biofeedback using RV and surface electromyography (sEMG) to complement conventional therapy upper limb post-stroke patients. The system was developed in accordance with the requirements in the design phase and design, considering the patient's functional limitations, residual skills, a motivating environment and ease of use. He is able to provide feedback of sEMG, the result (through the score in the game) and the angle described. The three games developed, aims to motivate the patient to perform elbow extension movements and meet the principles of motor learning. To develop and evaluate the system experiments were conducted with individuals without motor or neurological involvement. Analyzing the graph obtained by the myoelectric signal filtered and rectified is possible to observe a co-contraction of Bícpes Brachial (BB) and Trícpes Brachial (TB). When we analyze the results of feedback, the data show that and were not found significas differences (p = 0.9216) when playing with the dominant arm (BD) versus non-dominant (BND). However a significant difference is observed when comparing both arms versus BD (BD / BND) and p = 0.0003 vs. BND BD / BND, p = 0.0018. The results of the evaluations through the System Usability Scale (SUS), in the experiments of stage 2 (E2) was ± 81.4; SD ± 3.4 and stage 3 for participants (E3 / P) ± 82.5; SD ± 14.3 and rehabilitation professionals (E3 / PR) ± 81.1; SD ± 7.4). In Goal Attainment Scale (GAS), E2 was ± 73.3; SD ± 2.7; E3 / P ± 72.2; SD ± 6.1 and E3 / PR ± 73.5; SD ± 2.7. The results show good acceptance of the system by the users, and that the objectives expected during the use of the system have been achieved.
Análise de VEGF-C, COX-2 e IL-17A como biomarcadores do carcinoma epidermóide de cabeça e pescoço
(Universidade Federal do Espírito Santo, 2017-02-23) Bastos, Alliny Cristiny da Silva; Gouvêa, Sônia Alves; Guimarães, Marco Cesar Cunegundes; Santos, Hérica de Lima
Head and neck squamous cell carcinoma (HNSCC) is one of the most frequent types of cancers presenting a high mortality rate and morbidity rate for individuals worldwide. Although several studies present possible biomarkers (BM) for HNSCC there is no specific BM established for this disease. The objective of this work was to evaluate the role of VEGF-C, COX-2 and IL-17A as possible BM of HNSCC, since their high expression has been related to a worse prognosis of the disease. A cross-sectional clinical study, approved by the CEP/UFES Ethics Committee n°99.242/2012, was performed. Biological samples and clinical-pathological data from 124 patients with HNSCC and 29 healthy individuals were collected. The detection of VEGF-C mRNA and COX-2 expression was performed by qPCR trials on tumor tissues and normal tissue samples. The evaluation of VEGFC and COX2 protein expression was performed with serum from both HNSCC patients and a control group by Western blotting. The level of IL-17A (pg/ml) was measured by flow cytometry. Increased relative expression of VEGF-C mRNA in tumor tissue was observed in patients with lymph node metastasis (p=0,027) compared to without lymph node metastasis (p=0,043) and normal tissue samples (p=0,031);Variation of VEGF-C expression in serum of patients in advanced stages of the disease (III/IV) was observed (p=0,01) in comparison with patients in initial stages (I/II) (p = 0,03) and also the control group (p=0,003). COX-2 did not present a significant difference in any of the parameters of the biological samples analyzed. The level of IL-17A was higher in the serum samples from patients in the initial stages of HNSCC (I/II) (p=0,03), in smokers with the disease (p=0,04) and in those without regional lymph node metastases(p=0,04) when compared to the control group. VEGF-C and IL-17A were shown to be potential biomarker of HNSCC in serum samples, being a less invasive and more feasible way to evaluate the patient's condition, whereas for COX-2 new approaches are necessary to evaluate its role in HNSCC.

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