Análise de fatores de virulência relacionados à adesão e ao fenótipo de variante de pequenas colônias em cepas de Staphylococcus aureus isoladas de infecção óssea maxilofacial
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Data
2020-12-21
Autores
Soares, Dalliane Oliveira
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Universidade Federal do Espírito Santo
Resumo
Osteomyelitis is an infectious disease that affects bone tissue and has the most frequent etiologic agent Staphylococcus aureus. Osteomyelitis in the maxillofacial region has a significant impact on oral health in developing countries, has high morbidity, low cure rates and often the need for multiple surgeries that can lead to deformations and chronic sequelae, compromising the quality of life of patients, in addition to high hospital cost. Thus, this study is important, as it is proposed to elucidate some mechanisms involved in the pathogenesis of maxillofacial osteomyelitis. The aim of this study was to compare strains of Staphylococcus aureus isolated from maxillofacial osteomyelitis and asymptomatic nasal carriers for the detection of virulence genes associated with the expression of type I collagen#binding adhesives (cna) and sialoprotein (bbp), in addition to ability to form the SCVs phenotype (small colony variants). Twelve strains of S. aureus were analyzed, isolated from maxillofacial osteomyelitis and 6 from asymptomatic nasal carriers. For bacterial identification, phenotypic tests were used, such as: morphotintorial analysis, coagulase test, catalase and fermentation in mannitol agar. Selective pressure with the use of gentamicin antibiotic was used to identify the SCVs phenotype and amplify the adhesin genes cna and bbp using the PCR technique. Our results showed that virulence genes associated with the expression of adhesins bbp and cna were not determinants for the establishment of bone infections considering the analyzed strains of Staphylococcus aureus isolated from maxillofacial osteomyelitis and asymptomatic nasal carriers, suggesting that other mechanisms are involved in this process, as the manifestation of the SCV phenotype, which has shown to play an important role in the establishment and persistence of chronic suppurative osteomyelitis.
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Infecção óssea maxilofacial , SCVs , Adesinas bbp e cna