Biologia Vegetal

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http://repositorio.ufes.br/handle/10/17503

Programa de Pós-Graduação em Biologia Vegetal

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URL do programa: http://www.biologiavegetal.ufes.br/

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Ácidos húmicos extraídos do lodo de esgoto sanitário e seus efeitos em plantas
(Universidade Federal do Espírito Santo, 2014-02-27) Rocha, Lívia Dorsch; Dobbss, Leonardo Barros; Matsumoto, Silvia Tamie; Cuzzuol, Geraldo Rogério Faustini; Mazzeo, Dânia Elisa Christofoletti
The final disposal of sewage sludge has been presented as an environmental and public health problem. The sewage sludge is rich in organic matter but it also presents pollutants and pathogens. Thus, this work was extracted humic acid (HA) which is a compound resulting from the fractionation of humic substances that comprises a carbon group generated by the decomposition of organic waste that suffer resynthesis forming humus. Humic acid promotes many benefits in plants, such as growth cell elongation, stress tolerance, increased plasma membrane permeability. Based on these features, the present study aimed to evaluate the cytogenetic effects (Allium cepa test), physiological and biochemical in Oryza sativa culture treated with differents concentrations of humic acids derived from sewage sludge. Elemental characterization of HA was performed to define the doses (0,5; 1, 2 e 4 mM C L-1). After 20 days of treatment, samples of the material were taken and subsequently analyzed. The chemical characterization of HA indicated it as a good conditioner for cultures showing high rates of Carbon, Hydrogen and Nitrogen. No effect of toxicity, cytotoxicity, genotoxicity and mutagenicity of HA was observed. The results have shown significant increase of plant photosynthetic pigments on higher concentrations (2 mM C L-1 and 4 mM C L-1). In general the higher concentrations have presented more growth. Futhermore, there was an increase of ATPase expression in all treatments. And enzymes of oxidative stress increased relatively in differents concentrations. By the integration of these analyzes it was possible conclude that the humic acid from sewage sludge can be used as organic fertilizer as observed by the plant benefits showed in this work. Moreover, HA offers a good alternative for the disposal of sewage sludge residue, recycling the organic matter.
Acúmulo de nutrientes e carboidratos em populações da espécie Myrsine cariacea
(Universidade Federal do Espírito Santo, 2019-03-12) Leite, Kelvin de Jesus; Cavatte, Paulo Cezar; https://orcid.org/0000-0003-2963-7673; http://lattes.cnpq.br/8029279967950425; https://orcid.org/0000-0003-1830-3651; http://lattes.cnpq.br/3601125549486192; Martins, Samuel Cordeiro Vitor; https://orcid.org/0000-0002-2428-8649; http://lattes.cnpq.br/3010246075881689; Werner, Elias Terra; https://orcid.org/0000-0003-2549-7626; http://lattes.cnpq.br/3268068963656927; Garbin, Mario Luis; https://orcid.org/0000-0003-4137-2102; http://lattes.cnpq.br/4873285090929011
In dioecious species, female and male individuals may present differences in some ecophysiological characteristics. The sexual dimorphism in characteristics related to the absorption and use of resources is expressed mainly during the reproductive period, because the investments of the male individuals are limited to the flowering, whereas the feminine, in addition to producing the flowers, had to invest a great quantity of the resources available for fruit production. However, variations in environmental conditions, as occurs along an altitude gradient, affect the ecophysiological performance of plants, and may modify the differential demand of resources between the sexes and, consequently, the accumulation of nutrients and carbohydrates. The objective of this work was to examine the differential accumulation of nutrients and carbohydrates in individuals of eight populations of the Myrsine coriacea dioecious species, located along an altitude gradient in the Atlantic Forest. At the end of the fruiting period, leaf samples were collected from twelve individuals (six female and six male) from each of the eight populations analyzed. Analyzes were performed to determine the concentration of nutrients and non-structural carbohydrates in leaf tissue. Soil samples were collected in the canopy projection of each individual to study the relationship between soil chemical conditions and nutrient accumulation in leaves. The accumulation of carbohydrates and nutrients was similar between the sexes, with significant differences only between the populations. The population located at the highest altitude (1774 m altitude) had a higher accumulation of soluble carbohydrates (glucose and fructose) and less accumulation of starch, potassium and boron in relation to other populations. A negative relationship was observed between altitude and nitrogen, potassium and boron leaf accumulation; for the accumulation of iron and calcium the relation was positive. The availability of nutrients in the soil was significantly different between the sexes, but this response is dependent on the conditions of each population. The concentrations of potassium, calcium and magnesium in the leaves were associated with the availability of these macronutrients in the soil. However, the availability of phosphorus in the soil was not associated with the concentration of this macronutrient in the foliar tissues. It should be noted that the differences in soil chemical conditions verified between the sexes were not accompanied by significant variations in the accumulation of nutrients in the leaves. Thus, our findings indicate that male and female plants of M. coriacea must present variations in the mechanisms of production / absorption, assimilation and / or allocation of carbohydrates and nutrients, as a way of supplying differential reproduction costs, maintaining the same carbohydrate concentration and nutrients in leaf tissues.
Algas epilíticas em um reservatório tropical raso (Vitória, ES): estrutura e dinâmica em diferentes escalas temporais
(Universidade Federal do Espírito Santo, 2008-12-18) Cavati, Bruna; Silva, Diolina Moura; Fernandes, Valéria de Oliveira; Dias Júnior, Camilo; Rodrigues, Liliana
This research, developed in the Captation Lagoon of the water trucks supplying center of the City Prefecture of Vitória, a shallow tropical reservoir, aimed to evaluate changes in the structure and dynamics of the epilithics algae community in different temporal scales (succession and hydrological periods) in artificial limestone substratum. An experimental structure was confectioned, serving of support for limestone substrata, and implanted in the sampling site (located in the middle of the lagoon). The experiment had lasted 61 days in each season (dry and rainy). The measured abiotical variables were: wind velocity, light intensity, site depth, Secchi transparency, eufotic zone, air and water temperature, electric conductivity, total solids in suspension, turbidity, dissolved oxygen, pH, nitrite, nitrate, ammoniacal nitrogen, total nitrogen (kjeldahl), ortophosphate, total phosphorus dissolved and silica. The climatological data (precipitation and air temperature) had been obtained from the Incaper meteorological station. The structure and dynamics of the community had been evaluated based in the following attributes: specie richness, density, abundance, dominancy, diversity, evenness and biovolume. The chlorophylls a, b and c, carotenoids and phaeopigments concentrations had also been quantified in the epilithic algae. The lowest nitrogen-nutrients and electric conductivity values and the highest turbidity and phosphorus concentration values were reported in the higher precipitation period. According CONAMA resolution nº357/2005, the Captation lagoon can be classified as “water class 2” (destinated to parks and garden irrigation) and only the phosphorus values are higher than the resolution stardard quality values. 115 taxa were reported, with qualitative predominance of the Class Cyanophyceae, Chlorophyceae and Bacillariophyceae. In both periods, the density, biovolume and pigments concentrations were crescent; however, the most expressive density and pigments concentration increase occured in the dry season, as long as the biovolume increase occured in the rainy season. The diversity and evenness index showed temporal variations in short ans long-term. The succession standard was, quantitatively: initial colonization by Chlorophyceae, which was substituted by Cyanophyceae in the intermediary and final stages. Bacillariophyceae contributed in the intermediary stages, mainly in the dry season. Chlorophyceae showed the highest revelance to biovolume. Short-term community variability was determinated by autogenic process (competition and resources limitation), which were more accented in dry season. Pluviometric index and nitrogen and phosphorus-nutrients concentrations variation were determinants in long-term. The specie richness, density and pigments concentrations showed more sensible answers to nitrogen and to the disturbance features than the biovolume, which was masked in the rainy season by the taxonomic representation
Alocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperatura
(Universidade Federal do Espírito Santo, 2019-11-06) Gasparini, Xismenia Soares da Silva; Cuzzuol, Geraldo Rogerio Faustini; https://orcid.org/; http://lattes.cnpq.br/6127436626143032; https://orcid.org/; http://lattes.cnpq.br/; Aquije, Gloria Maria de Farias Viegas; https://orcid.org/0000-0002-8810-7253; http://lattes.cnpq.br/; Milanez, Camilla Rozindo Dias; https://orcid.org/; http://lattes.cnpq.br/1288447995978779; Gomes, Marcos Thiago Gaudio; https://orcid.org/; http://lattes.cnpq.br/9903805899337418; Cavatte, Paulo Cezar; http://lattes.cnpq.br/8029279967950425
PPaubrasilia echinata is a tree species originating from the Atlantic Forest. This species has different morphotypes, among them the small and medium that differ in morphology and ecological habit. The small morphotype is described as an ombrophilous plant, while the medium morphotype shows heliophilic behavior. From the climate reports released, it is noted that environmental disturbances may occur as a result of these changes. According to reports, for the northeast portion of the Atlantic Forest is expected intense warming and reduction of rainfall. For the south / southeast portion, trends indicate warmer weather, with intensification in rainfall patterns. The objective of this study was to evaluate the combined effect of vapor pressure deficit (DPV) and temperature on carbon allocation, as well as non-enzymatic antioxidant responses (flavonoids, phenols and MDA) as mechanisms of tolerance to these climate changes. Therefore, plants of small and medium morphotypes were submitted to four different controlled environments (A), being A1- low DPV and temperature (0.7 KPa and 25.9 ° C); A2- low DPV and high temperature (31.6 ° C and 1.0 KPa); A3- high DPV and temperature (32.6 ° C and 3.1 KPa) and A4- high DPV and temperature (26 ° C and 1.7 KPa). The analysis of soluble sugars (AS), sucrose, reducing sugars (AR), starch and wall polymers (cellulose, hemicellulose and lignin) were performed in the three organs (leaf, stem and root) of plants of both morphotypes. Already the analyzes referring to the non- enzymatic oxidant system were performed in the leaves. Both morphotypes showed, in the A3 environment, higher concentration of leaf AS in relation to the stem and root. This result presented higher RA concentration and lower leaf sucrose concentration, since this carbohydrate can be used as osmoregulator, besides being the main translucent photoassimilate in plants and can be used in other reactions. The roots of the small morphotype, under elevated temperature, presented higher root concentration of sucrose compared to the other environments. In both morphotypes, the RA concentration was higher in leaves, especially in A3. Under high DPV, especially in A3, the small morphotype showed higher root starch concentration and lower leaf concentration. In the mean morphotype,. starch allocation was higher in leaves than in other organs. The highest starch concentration is related to storage organs (seeds, tubers, roots ...) and its lower leaf concentration is related to stress conditions. The high concentration of lignin in the roots of both morphotypes may favor water transport, besides protecting plant tissues from chemical / biological degradation. The highest concentration of cellulose and hemicellulose was observed in the medium morphotype A1 stems, and the opposite for the small morphotype. Regarding the oxidative reaction, the small morphotype presented higher content of H2O2 in A4, where the lipid peroxidation, indicated by the higher MDA content, was higher. The same result was observed for the average morphotype in A1. Under these conditions, the non enzymatic antioxidant system was inefficient due to the lower concentrations of phenols and flavonoids. In higher temperature environment, the small morphotype (A2) and the medium morphotype (A3) presented lower concentration of H2O2 and MDA. This result may suggest less cellular damage due to lower lipid peroxidation. Thus, it can be concluded that A1 and A4 appear to be stressful conditions for the small and medium morphotype, respectively. However A2 and A3 can be considered favorable environments, respectively, for the small and medium morphotypes.
Análise comparativa dos teores de fenois e flavonoides e de atividades biológicas de cinco genótipos clonais de Schinus terebinthifolius Raddi (aroeira)
(Universidade Federal do Espírito Santo, 2020-02-27) Lima, Savio Cabral Lopes de; Batitucci, Maria Do Carmo Pimentel; https://orcid.org/0000-0002-3485-4448; http://lattes.cnpq.br/0010148251489155; https://orcid.org/; http://lattes.cnpq.br/3103652143909015; Franca, Hildegardo Seibert; https://orcid.org/0000-0001-6129-8793; http://lattes.cnpq.br/1284874997224988; Dutra, Jean Carlos Vencioneck; https://orcid.org/0000-0003-3893-9470; http://lattes.cnpq.br/4101176432227035
The Schinus terebinthifolius Raddi belong to the Anacardiaceae family, popularly known as pink pepper, is native from South America, and is present in traditional medicine and knowledge as a medicinal plant with healing and anti-inflammatory potential. The fruits are used as a culinary condiment in Brazil and even more so in European countries and North American, to which they are exported, therefore, they have significant economic importance and the present study aims, through analysis, to differentiate and select the genotypes that stand out. The leaves and the bark are used in infusions and decoctions by people without any evaluation and safety in consumption, which can cause damage to health, since studies have found a series of secondary metabolites with beneficial and harmful actions to human health, among them flavonoids, tannins, alkaloids, coumarins and terpenes. In this context, four different antioxidant tests of the ethanolic extract of five clonal genotypes provided by INCAPER (Institute of Rural Research and Extension) were carried out, based on the results obtained, a low antioxidant activity of the extracts is observed, except for the FRAP test, in which the IC50 values are close to that found in the standard. In the cell viability assay (MTT), significant cytotoxicity was found in the 48-hour exposure treatments, especially regarding lymphocytes. A data worth mentioning is that the concentration of 10 µg / mL of the extracts that promotes a proliferative action of Sarcoma 180 cells, after exposure for 48 hours. The anti-cytotoxic test shows that all genotypes have good preventive activity, as well as demonstrating a significant increase in cell viability after treatment in all concentrations used. Concluding then, that the extract of the pink pepper fruit, in certain concentrations, can be cytotoxic and even cause the proliferation of tumor cells, however, they also seem to work in preventing damage to cells as well as in cell recovery
Análise da composição química e das atividades biológicas de Schinus terebinthifolia Raddi (aroeira) submetida a diferentes tipos de adubação
(Universidade Federal do Espírito Santo, 2020-02-28) Aleluia, Rene Lemos; Batitucci, Maria Do Carmo Pimentel; https://orcid.org/0000-0002-3485-4448; http://lattes.cnpq.br/0010148251489155; https://orcid.org/0000-0002-8531-7044; http://lattes.cnpq.br/6002113805684020; Ventura, Jose Aires; https://orcid.org/0000000314221739; http://lattes.cnpq.br/8687116881326074; Kuster, Ricardo Machado; https://orcid.org/0000000289615348; http://lattes.cnpq.br/4149814906786366
Schinus terebinthifolia Raddi (Anacardiaceae) is a plant native to South America, popularly known as Brazilian peppertree. It is one of the most used plant species in traditional medicine and gastronomy. The use of aroeira and its products is still based on extractivism, which does not guarantee uniformity regarding its chemical composition and the biological activity resulting from those compounds, due to the interference of environmental factors. The objective of this paper was to evaluate the influence of different types of fertilization on the production of secondary metabolites of S. terebinthifolia plants regarding the biological activities exhibited by the ethanol extracts produced from the leaves and fruits of those plants. The plant material used in the present study was collected in an experimental plantation at INCAPER, Linhares - ES. Different fertilization conditions were used, with the following characteristics: T1 = control; T2 = 100g of NPK fertilizer; T3 = T2 + 2g of boron (H3BO3); T4 = T2 + 4g zinc (ZnSO4); T5 = T2 + 2g of boron (H3BO3) + 4g of zinc (ZnSO4), and T6 = 500g of organic fertilization (bovine manure). After the collection, the plant material (leaves and fruits) of each treatment was dried, crushed and crude ethanolic extracts were produced with them. Leaf and fruit samples were evaluated for their chemical nutrient profile. All the extracts produced were evaluated for their chemical profile using Mass Spectrometry (ESI (-) FT-ICR MS and MS / MS), phytochemical composition (phenols, flavonoids and tannins), antioxidant activity (ABTS, DPPH, FRAP and Fe2+ chelation), cytotoxicity and anti-cytotoxicity in human lymphocytes and antiproliferative effect in in vitro Sarcoma 180 cells (MTT assay) and allelopathic potential (Allium cepa test system). The analysis of the chemical content of the plant material of S. teribinthifolia revealed that the leaves were the organs that showed the greatest alteration in the content of macro and micronutrients, as they contained low levels of P, Fe, Cu, Zn and Mn. The fruits of plants fertilized with T1, T2, T3, T4 and T6 showed a lack of Zn and Mn, but a high content of copper (Cu). Spectrometric analyses identified different classes of substances in the ethanol extracts of fruits and Brazilian peppertree leaves, with emphasis on anacardic acids and their derivatives. The extracts of fruits and leaves of S. terebinthifolia showed different responses in the phenolic quantification tests and in the antioxidant tests, with T1F and T6F having higher content of phenolic compounds. Multivariate analysis (PCA) demonstrated that the presence of those bioactive compounds influenced better responses of these extracts in antioxidant action, when evaluated by ABTS, DPPH and FRAP tests. However, in the Fe+2 chelation test, fruit extracts from treatments T3 and T5 were more efficient. In the evaluation of cell viability in human lymphocytes, most extracts tested showed cytotoxic activity at the concentrations and times evaluated, with the T2 treatment being the one with the greatest selectivity of action in relation to tumor cells. In the anticytotoxic evaluation, in the simultaneous treatments and in post-treatment, the extracts of T1F and T6F demonstrated a protective capacity to human lymphocytes against cisplatin. Regarding the allelopathic potential (AI), all extracts tested interfered in the germination of seeds exposed to them, as well as in the parameters of germination (GSI) and root growth (RML and RGSI)
Análise da expressão gênica e atividade enzimática antioxidante em Passiflora edulis SIMS sob diferentes concentrações de alumínio
(Universidade Federal do Espírito Santo, 2012-02-29) Pretti, Irany Rodrigues; Batitucci, Maria do Carmo Pimentel; Ferreira, Marcio Alves; Fontes, Renata Venturim
The passion fruit is cultivated in almost all the brazilian territory. The Espírito Santo State currently ranks fourth in production in the country. The production of Espírito Santo, Passiflora edulis, is expanding, given that in recent years there has been an increase in planted area of 500 ha of passion, in 1990 to 2,429 ha in March 2008, reaching 2010 in December, 2800 ha planted. However, most of the production in Espírito Santo is still concentrated in the northern region of the state, especially the municipalities of the Jaguaré and Sooretama. While the numbers show progress in the production of fruit, the Espírito Santo is characterized by acidic soils and high aluminum content. Among the responses triggered by exposure to aluminum are: inhibition of root growth, inhibition of cell division, nutrient deficiency, and the activation of signaling pathways and changes in level of protein and RNA transcripts. Thus, it becomes important to study the components of the antioxidant defense system in plants undergoing exposure to aluminum. The ROS can pose serious threat to the cell, one of the responses to environmental stresses such as toxic aluminum. However, few studies have reported the effects of Al on the plant canopy. Therefore, the objective of this study was to evaluate the response of the antioxidant system P.edulis under such conditions. The study of the oxidative stress induced by aluminum showed that the CAT and SOD gene expression of was increased in plants in the nutrient solution and only cv. FB100 this increase was accompanied by increment enzymatic activity of SOD, indicating the higher efficiency of the removal of ROS. Plants grown in the field showed that the expression of antioxidant enzymes in the crop with high aluminum content was not activated at the time of collection, despite the high activity of SOD and APX. This response was possibly determined by the extended time to which the plants were exposed to stress.
Análise do desenvolvimento de sementes de mamoeiros (Carica papaya L.) infectados com Papaya ringspot vírus e Papaya meleira vírus
(Universidade Federal do Espírito Santo, 2015-02-25) Salgado, Raquel Pereira Passos; Fernandes, Patricia Machado Bueno; Costa, Adelaide de Fátima Santana da; Rodrigues, Silas Pessini
The papaya (Carica papaya L.) is a fruit tree very important for the State of Espírito Santo, which is the second largest producer and the largest exporter of papaya in Brazil. However, the diseases papaya sticky desease (or meleira) and mosaic of papaya, caused, respectively, by Papaya meleira virus (PMeV) and Papaya ringspot virus-P (PRSV-P), are the main limiting factors. The seeds of Carica papaya are intermediate, for tolerance to desiccation, its reserve tissue is the endosperm, has asynchronous germination due to the presence of inhibitors in the sarcotesta region. It is known that viral transmission by seeds is a very important route for the spread of PMeV, and PRSV-P is not typically transmitted through this route. This study aims to evaluate if there is interference of PMeV and PRSV-P on the germination of seeds from infected plants. This research found that the PMeV causes the reduction of seeds biomass, germination rate, growth and biomass of the plants, and damage in the surface structure of the embryos. The PRSV-P virus demonstrated does not compromise any of the parameters. In conclusion, PMeV virus affect the formation and development of the seeds of infected papaya cv. Golden plants, and the PRSV-P virus does not cause any damage to seed as the PMeV virus does
Análise do nível de vulnerabilidade dos reservatórios de carbono em arbóreas funcionais de restinga e manguezal às variações do clima
(Universidade Federal do Espírito Santo, 2018-03-08) Knupp, Karina Tiussi Batisti; Cuzzuol, Geraldo Rogério Faustini; Menezes, Luis Fernando Tavares; Saiter, Felipe Zamborlini
Changes in climate have been taking place with implications for thermal regimes and precipitation. How plants will respond to these changes is still uncertain. In particular carbon pools, which account for up to 80% of the structural mass of plants. It is interesting to investigate the vulnerability level of some species of the coastal formations of restinga and mangrove of the Mata Atlântica biome of Greater Vitória - ES in relation to the variations of the climate. Therefore, the carbon storages contents in leaf and drain (stem) organs of Clusia hilariana, Ocotea notata and Protium icicariba restinga, and Avicennia schaueriana, Laguncularia racemosa and Rhizophora mangle were evaluated in the autumn of 2016 and summer of 2017. Data were related to temperature and precipitation values. Non-structural carbohydrates (starch, sucrose and total soluble carbohydrates) and cell wall structural polymers (cellulose, hemicellulose and lignin) were determined. Data were submitted to analysis of variance and Tukey test at the 5 % level (P <0.05) of significance, when necessary. The summer showed more precipitation. In the autumn of 2016 the average temperature was 27 ° C and in the summer of 2017, 25 ° C. The species of the two coastal formations responded differently to climate variations. In the mangrove, the concentration of cellulose in stem and leaf varied, being higher in the summer. In the restinga, the concentration of cellulose and starch in stem and leaf was higher in the summer. Similarly, in the restinga, the non-structural sucrose carbohydrate from the leaf, was also higher in summer. The concentration of lignin in the restinga was higher in the autumn. The restinga, due to changes in the concentrations of its carbon storages to temperature and precipitation variations between autumn and summer, can be considered, with greater vulnerability to temperature and precipitation changes in which ecosystems are being submitted.
Análise fitoquímica e avaliação das antioxidante, antimutagênica e citotóxica do estrato hidoalcoólico de Coriandrum sativum L.
(Universidade Federal do Espírito Santo, 2016-03-28) Santos, Patricia Carara dos; França, Hildegardo Seibert; Batitucci, Maria do Carmo Pimentel; Corte, Viviana Borges; Jamal, Claudia Masrouah
Coriandrum sativumL., known as coentro, is included in the Apiaceae family and It is a vegetable originating from European and African Continent. Flowers, leaves and fruits are commonly used of this plant. In addition it plays an important role in folk medicine and It is recommended for treatment of various diseases. This plant is rich in phenolic compounds that are related to Its antioxidant effects. The substrate involved in medicinal plant cultivation process can interfere in the production of secondary metabolites with bioactive principles . Another important factor in the production of metabolites is the development stage in which the plant is and there is no scientific works with this approach involvingC. sativum. The aims of this study was evaluate the influence of fertilization on the production of secondary metabolites in two stages of development of Coriandrum sativumand relate these conditions with quimioprotetor, antimutagenic and antioxidantpotential of hydroalcoholic extract of the leaves of thisplant. Theplants were grown in the Venda Nova do Imigrante/ES –Brazil under the organic fertilizer regimes (bovine manure) and chemical fertilizer (NPK), field conditions, and was evaluated two stages of development (vegetative and flowering). Aerial parts were dried and subjected to maceration in 70:30 ethanol/water (vol/vol) to obtain the crude extract which were underwent a phytochemical characterization by colorimetric methods and mass spectrometry. All cultivation conditions showed that the extracts had same phytochemical classes of metabolites (coumarins, flavonoids and steroids) andthe mass spectrometry indicated similarities between the extracts evaluated. The crude extract of C. sativumin the vegetative stage and chemical fertilization showed better antioxidant activity, according to the DPPH test when compared to other treatment groupsandthe extracts obtained from vegetative stage of the 8plantsunder different cultivation conditionsshowed a reduction in the micronucleus frequencyin relation to the positive control in the pretreatment and simultaneously assay.
Análises morfofisiológicas de plantas de pau-brasil (Caesalpinia echinata Lam.) cultivadas em pleno sol e em sombreamento natural
(Universidade Federal do Espírito Santo, 2013-02-26) Gama, Vinícius Novo; Cuzzuol, Geraldo Rogério Faustini; Corte, Viviana Borges; Gonçalves, José Francisco de Carvalho
Caesalpinia echinataLam. is a endangered tree of the Atlantic Florest. Published data has demonstrated that this specie threedifferent leaf morphological variants, and variant small leaflet considered a secondary classification plant forest succession, in contrast with the results of growth medium leaflet variant observed in the field. The central aim of this study was to characterize some morphological, biochemical and physiological leaf morphotype average C. echinata contrasting under conditions of irradiance. This work was conducted at the Reserva Natural da Valein the municipality of Sooretama, ES. We analyzed average leaflets of fully expanded leaves us third-fourth of young plants of Brazil wood in two different light conditions: full sun and natural shade. Plants in full sun showed greater efficiency in capturing light energy, followed by a photochemical better utilization and higher net carbon assimilation, which possibly resulted in a higher content of soluble carbohydrates. Because of the imbalance photosynthetic presumed to have been an excess plant photorespiratory activity in shading in an attempt to prevent the formation of free radicals by reducing compounds formed. The energy that should be being used in photochemical reactions and / or biochemical plants to shading was, for the most part, dissipated as heat or re-emitted as fluorescence translocated to the production of antioxidative defense compounds of secondary metabolism. Therefore, the high light condition provided greater growth of population exposed to full sun in relation to plants exposed to low light. The morphology and anatomic levels of photosynthetic pigments of plants exposed to the sun presented in this study also indicate a strong tolerance to this variant of intense light condition. The results presented in this paper show that the direct solar radiation is configured as a condition more suitable for the planting of shading variant mean leaf of Brazil wood. Given these differences in morphology and ecophysiological variants of the species C. echinata, a restructuring is necessary taxonomic species in possible sub-specific taxa, for thus reestablish anew classification of Brazil wood in forest succession, and thus provide support for proper planting and management of this plant in reforestation programs
Aspectos ecofisiológicos das cianobactérias em uma lagoa costeira urbana (Lagoa Juara, Serra, ES)
(Universidade Federal do Espírito Santo, 2015-02-24) Alves, Fernanda Brêda; Fernandes, Valéria de Oliveira; Dias Júnior, Camilo; Suzuki, Marina Satika
This study aimed to evaluate the cyanobacterial community structure analyzing taxonomic richness, composition, total density (individuals / mL), abundance, dominance of species beyond their biomass in Juara pond in spatial scale, vertical and temporal. Two samples were performed, one in the dry season (Sept / 2013) and another held in the rainy season (Mar / 2014) at three sampling stations located in a longitudinal gradient in the lagoon, representing regions near the mouth of streams (including those backers of matter organic resulting from domestic effluent discharge "in natura" is primarily via stream Laranjeiras), the sea andthe deployment area of the cages. Sub-surface samples were collected at depths of 50% brightness 1% light water column, always in the morning. Qualitative samples were collected with a plankton net of 20 microns and quantitative bottle with van Dorn. The cyanobacterial community structure was evaluated based on its key attributes: taxa richness, total density, abundance and dominance. The biovolume was calculated based on geometric shapes approximating the shape of the cell. Were determined: temperature (air / water), euphotic zone depth, pH, dissolved oxygen (DO), turbidity, electrical conductivity (EC) and major nutrients (silicate, nitrate, ammonia nitrogen, total nitrogen, orthophosphate, total phosphorus). We recorded 43 species of cyanobacteria, belonging to 17 genera, 8 families and 4 orders, 3 genres confirmed in literature as microcystin producers. The total density of cyanobacteria showed higher values in samples influenced by precipitation with no differences between the EA1 and EA2 station, just sampling station AE3 significant difference between the period of drought and rain, with higher density figures in the dry season. The biovolume data were higher in the rainy season and EA2 sampling station. The vertical variation of biovolume showed a significant difference only in the sampling stations EA1 and EA3, with uniform EA2 station at different depths. So the Microcystis genus had a higher contribution in terms of biovolume. The microcystin produced mainly by this genre was present in the aquatic environment, both in the dry season as in the rainy, with values within the parameters allowed by law. The values of total phosphorus, total nitrogen and nitrate showed temporal and spatial variation. The vertical variation was slight in the depths of the three sampling stations. The highest recorded concentrations of orthophosphate, total phosphorus, total nitrogen and nitrate, especially in EA2 and EA3 stations. The EA2 and EA3 stations showed limnological and biological characteristics of similarity. Both stations suffer human impact due to intensive fish farming system and through the discharge of domestic sewage "in natura" via stream Laranjeiras respectively. The Juara pond has multiple uses, and the development of subsistence activity as intensive fish farming, this practice together with the impacts associated with the injection of wastewater, showed signs of changes regarding the environmental conditions in the pond leading to the development of species-producing cyanobacteria microcystin. The presence of this toxin at high levels may impair the use of the pond, especially intensive farming of fish for consumption and leisure and recreation.
Aspectos fisiológicos em Mangifera indica L. produzidas com rejeitos de mineração e tratadas com fertilizante organomineral
(Universidade Federal do Espírito Santo, 2020-02-13) Silva Junior, Romario de Oliveira ; Silva, Diolina Moura; https://orcid.org/000000033885280X; http://lattes.cnpq.br/0341541450627705; https://orcid.org/0000-0003-2044-3661; http://lattes.cnpq.br/2462240761208325; Araujo, Wagner Luiz; https://orcid.org/0000-0002-4796-2616; http://lattes.cnpq.br/8790852022120851; Cavatte, Paulo Cezar; https://orcid.org/0000-0003-2963-7673; http://lattes.cnpq.br/8029279967950425
This work aimed to evaluate the physiological performance, in the field, of two cultivars of Mangifera indica L. (‘Palmer’ and ‘Rosa’) produced on substrates containing 0%, 20% and 50% of mining tailings. During field cultivation, no longer under the direct influence of mining waste, the plants were subjected to two organomineral fertilization treatments, dividing the crop into unfertilized and fertilized plants. The physiological responses were obtained in different experimental periods: 30, 150 and 300 days from the beginning of fertilization (DAF). The levels of leaf pigments, the kinetics of chlorophyll a fluorescence, the levels of non-structural carbohydrates and the activity of enzymes in the anti-oxidant system were analyzed. The results showed that the production of seedlings with mining tailings did not change the concentration of chlorophylls and carotenoids, but direct relationships were found with organomineral fertilization. On the other hand, chlorophyll a fluorescence analyzes at 30DAF showed lower photochemical performance in both cultivars that had a higher concentration of tailings in the substrate, during the seedling phase. The OJIP curves and the JIP test showed damage linked to photosystem II. There was less performance of photosystem II (PIABS) due to the drop in electron flow and electron transport performance (ΨE0 and φE0). In plants fertilized with the organomineral product, the best photochemical performance was clear. There was greater absorption and energy capture (ABS / RC and TR0 / RC), greater efficiency and efficiency in electron transport (ΨE0 and φE0) and in the performance index that represents electron transport to the final FSI acceptors (PITOTAL). At 150 and 300 DAF, the OJIP curves and the JIP test showed a different picture than what was observed at 30 DAF. The plants of the cultivars Palmer and Rosa showed a photochemical 'stability', however the fertilized plants showed recovery in less time. The results obtained by the analysis of chlorophyll a fluorescence also allowed to observe a better photochemical performance in cv. Rosa. There was no difference in the levels of non-structural carbohydrates in both cultivars or in the different treatments. Similar to the results obtained in the fluorescence kinetics of chlorophyll a, at 30 DAF the enzymes catalase, ascorbate peroxidase and gauiacol peroxidase had greater activity while at 150 and 300 DAF these enzymes had less activity. Finally, it is concluded that organomineral fertilization, in fact, promoted greater physiological recovery in less time, in addition to promoting better physiological performance for mango plants
Atividade fotoquímica em folhas do mamoeiro Carica papaya L. durante o período reprodutivo e caracterização da ontogenia dos frutos
(Universidade Federal do Espírito Santo, 2007-12-14) Santos, Ketney Tonetto dos; Oliveira Júnior, Luis Fernando Ganassali de; Silva, Diolina Moura; Schneider, Edgar; Aguilar, Marco Antonio Galeas
In this work the photochemistry activity in leaves of plants of the papaya tree Carica papaya L. cv. Golden was evaluated fotnightly in a commercial plantation. The measures had beginning in the anthesis and concluded after the first crop (January for junho/2007). The kinetics of the emission of the fast fluorescence of the chlorophyll was she obtained with aid of a portable fluorometer (Handy-PEA, Hansatech, UK) using leaves previously adapted to the darkness. The results showed that the alterations happened in the apparel photosynthetic were more severe in the month where there was an abrupt fall in the precipitation. Low values of the initial fluorescence (F0), maximum fluorescence (FM) and of the revenue quantum potential maximum of the PS II (FV/FM) they indicated the occurrence of chronic photoinhibition in this period. For they be still scarce the studies on the chemical and biochemical changes that happen starting from the ontogeny of the fruits of the papaya tree, in this work it was also evaluated some physical changes, chemistries and biochemistries in the fruits of this the anthesis to the matureness. The activity of pectinametylesterase (PME), enzyme that prepares the substratum so that the other enzymes pectinolíticas acts in the process of matureness of these fruits, it increased starting from 120 DPA in the same way that the index of SS. On this same occasion it happened a decrease of the pH of the cellular juice and of the titratable acidity (ATTN) contained in the pulp of the fruits. These results confirm the participation of the PME, the presence of the acids organic and the beginning of the degradation of the it seems cellular before even of the crop.
Atributos funcionais como resposta à plasticidade de Protium heptaphyllum (Aubl.) Marchand em área de restinga em condições de inundação do solo
(Universidade Federal do Espírito Santo, 2022-12-22) Jesus, Vera Lucia Pereira de; Menezes, Luis Fernando Tavares de; http://lattes.cnpq.br/4502113593775550; http://lattes.cnpq.br/9896749366602962 ; Almeida, Stefano Zorzal de; http://lattes.cnpq.br/3680096174839424; Antar, Guilherme de Medeiros; http://lattes.cnpq.br/0400818944403964
Functional attributes are the characteristics of an organism that were inherited in evolution and expressed according to the environment in which it is found. Based on this, the leaf characteristics of Protium heptaphyllum (Aubl.) Marchand were investigated in two restinga plant formations, one floodable and the other not, through the biometry of Protium leaves, stomatal density and chlorophyll pigments. Ten individuals of P. heptaphyllum were selected per area and 20 leaves were collected from each one for biometric analysis. The criterion for choosing the individual was based on the height and diameter of the species and its occurrence in the two restinga areas (flooding and non-flooding), in addition to being the only one of the genus Protium present in these areas. From the collection of leaves, leaf area and specific leaf area, fresh and dry mass, leaf and petiole thickness, leaf and petiole length, sclerophyll index, stomatal density, chlorophyll a, b and carotenoids were obtained. Mean values and the standard deviation of each biometric parameter were determined, considering the significant values between the two areas. Comparing the flooded and non-flooded areas, the results showed that there was no significant difference for leaf thickness and for chlorophyll a, b and carotenoid pigments. Stomatal density was higher in non-flooded. The attributes leaf area, specific leaf area, fresh mass, dry mass, petiole thickness, leaf and petiole length and sclerophily index were higher in the floodplain area. It is concluded that the biometric parameters that were most relevant represent the ability of P. heptaphyllum to adjust its morphological characteristics as a function of water stress, proving its plasticity, as well as readjusting its mechanisms as found in the chlorophyll contents, which in the two areas, did not change significantly.
Atributos quantitativos de espécies arbóreas pioneiras e não pioneiras da Floresta Atlântica
(Universidade Federal do Espírito Santo, 2015-02-25) Macieira, Bernardo Pretti Becacici; Cuzzuol, Geraldo Rogério Faustini; Falqueto, Antelmo Ralph; Musa, Bruna Cersózimo Arenque
The succession classification is based on very inconsistent quality attributes. The present study aimed to quantifysome phenotypicaspects in pioneer and non-pioneer tree species of the Atlantic Forest in the state of Espírito Santo. Leaves and stem segments of three pioneer species (Senna multijuga var. verrucosa, Miconia cinnamomifoliaandBixa arborea) and three non-pioneer species (Melanoxylon brauna, Carpotroche brasiliensisandNeoraputia alba)were analyzed. The pioneer species showed higher chloroplastid pigments; higher stomatal density and vessels elements diameters; higher glucose (6% of the dry mass –DM), fructose (2.3% DM) and sucrose contents (7.9% DM) found on the stem. Non-pioneer species showed higher leaf thickness, higher starch (2.8% DM) and cell wall polymers contents in their leaves (10% cellulose, 15% hemicellulose and 4% lignin DM). In the stem, the wall cell polymers contents in non-pioneer species (33% cellulose, 24% hemicellulose and 22% lignin DM) were higher than the pioneer species. The hemicellulose for both functional groups suggest that xylan (stem) and arabinogalactan (leaves) type. Whereas the soluble carbohydrates, starch and pigments are unstable molecules subject to environmental variations, we conclude that cell wall polymersare safer to characterize the functional classes. However, the lignin compound that was more varied between two functional groups (>100%) indicating that the content of polymer is more reliable quantitative attribute to identify pioneer and non-pioneer species from Atlantic Forest
Avaliação alelopática, mutagênica e fitoquímica de extratos vegetais de três espécies exóticas invasoras
(Universidade Federal do Espírito Santo, 2017-02-17) Moreira, Schírley Aparecida Costalonga; Batitucci, Maria do Carmo Pimentel; França, Hildegardo Seibert; Matsumoto, Silvia Tamie; Jamal, Claudia Masrouah; Belcavello, Luciano
Biological contamination is one of the most serious environmental problems nowadays and contributes to the extinction of native species. Studies aimed at understanding the mechanisms of invasion used by alien species are very important in the search for effective solutions to combat these species allelopathy is a promising field of investigation; however, the relationships between allelopathic effects and intracellular changes are rarely established. This study aimed to analyze the phytochemistry of the foliar ethanolic extract of Acacia mangium Willd (forest mangrove), Artocarpus heterophyllus Lam (jackfruit) and Eriobotrya japonica (Thunb.) Lindl (loquat) as well as infer about their allelopathic actions through four test organisms (Lactuca sativa, Allium cepa, Leucaena leucocephala e Urochloa brizantha) and its mutagenic potential using the A. cepa system. In allelopathic assay, seeds of test organisms germinated on Petri dishes covered with filter paper soaked with deionized water (negative control) or four concentration of each extract (1, 5, 10 and 50 mg/mL); were measured the germination index (GI), germination speed index (GSI), allelopathic index (AI), radicles growth speed index (RGSI), germination mean time (GMT), germination mean speed (GMS) and radicles mean length (RML). For mutagenic assay A. cepa seeds were submitted to continuous and discontinuous (acute and chronic) treatments in medium with deionized water, the herbicide trifluralin (1,9 µL/mL) - positive control -or one concentration of extracts and were measured the mitotic index (MI), aneugenic effect index (AEI), clastogenic effect index (CEI) and anomaly index (AI). A. mangium Willd extract presented in its composition saponins, triterpenes and tanins and affected the GI, GSI and AI of L. sativa, A. cepa and U. brizantha; in relation to GMT, L. leucocephala was the only specie affected. The GMS changed only in L. sativa. All species had RML and RGSI modified. The extract shown cytotoxic properties, since it reduced the MI of A. cepa without changed the AEI, AI and CEI. To A. heterophyllus Lam extract, was detected flavonoids, triterpenes and tanins and occurred significant changes in AI and GI to L. sativa, A. cepa e U. brizantha; the GSI was affected in L. sativa e U. brizantha, while GMT reduced in L. leucocephala e U. brizantha. RML and RGSI of all test organisms were altered. Besides reducing the MI, the extract caused an increase in CEI demonstrating that the observed allelopathic effects are reflections of both a cytotoxic and a genotoxic action. In turn, Eriobotrya japonica (Thunb.) Lindl extract shown flavonoids, steroids, saponins and alkaloids in its phytochemical profile and reduced the GI, GSI and AI in L. sativa, A. cepa and U. brizantha. There was a decline in GMS of L. sativa. The extract of Eriobotrya japonica (Thunb.) Lindl presented cytotoxic and mutagenic potentials and affected RML and RGSI of all test organisms. Of the three species tested, Eriobotrya japonica presented potential for the development of a Biological control for other alien species.
Avaliação da atividade alelopática, antioxidante, fotoprotetora e análises fitoquímicas do extrato de folhas de Euterpe edulis Martius
(Universidade Federal do Espírito Santo, 2020-03-20) Mariquito, Anderson; Corte, Viviana Borges; https://orcid.org/0000000254886578; http://lattes.cnpq.br/3694434981958328; https://orcid.org/0000-0003-4690-3865; http://lattes.cnpq.br/9625913121404212; Milanez, Camilla Rozindo Dias; https://orcid.org/0000-0002-9516-2031; http://lattes.cnpq.br/1288447995978779; Werner, Elias Terra; https://orcid.org/0000-0001-7781-4342; http://lattes.cnpq.br/3268068963656927; França, Hildegardo Seibert; https://orcid.org/0000-0001-6129-8793; http://lattes.cnpq.br/1284874997224988; Colman, Carine Coneglian de Farias; https://orcid.org/0000-0003-2967-3420; http://lattes.cnpq.br/9011970705908248; Seibert, Janaina Brandão; https://orcid.org/0000-0002-6112-5574; http://lattes.cnpq.br/5564487665806327
Euterpe edulis Martius (Palmeira Juçara) is a native Brazilian palm (Arecaceae), about which there are very few reports regarding its biological activity and chemical composition. The objective of this research was to perform bioprospecting and evaluate the biological potential of the ethanolic extract of leaves from E. edulis leaves and its fractions: hexane, dichloromethane, ethyl acetate, butanol and aqueous, determining the chemical classes present in these extracts. The germination index (GI), initial growth, enzymatic activity, content of chloroplast pigments and starch in seeds and seedlings of Lactuca sativa L. (lettuce) and Panicum maximum Jacq were evaluated at concentrations of 0.0, 0.25, 0.50, 0.75 and 1.0 mg/mL of these extracts and their fractions. Interpolating through a regression analysis, we observed that the hexane 0.75 mg mL and aqueous 1.0 mg/L fractions showed better results in relation to the GI variables (51.2 and 69.5%), germination speed index (3.45 and 4.55), allelopathy index (39.90 and 18.55) and root growth (2.87 and 3.11 cm), respectively. There was a significant difference in enzymatic activity for all treatments with the hexane fraction 0.75 mg/mL on lettuce and on colonization grass and for most aqueous fractions 1.0 mg/mL, with the exception of catalase on lettuce and peroxidase on colonial grass. Chloroplast pigments, as well as starch, increased significantly, but it was not enough to protect grass that had IG and initial growth negatively affected. The contents of phenolics, tannins and total flavonoids were evaluated. For the ethanolic extract and its polar fractions, the HPLC test was performed and for the non-polar fractions the GC-MS. The results obtained for the contents of phenols and tannins were significant for the crude extract and all its fractions, being thak acetate fraction having showed the best results. The best results for the phenolic content were obteined for the aqueous fraction. In the HPLC analysis, the analyzed patterns were not found. The GC-MS of the hexane and dichloromethane fraction demonstrated the presence of 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (Phytol) and vitamin E. The antioxidant activity was significant for the crude extract and its fractions in all concentrations for the ABTS, DPPH, FRAP and TAC-Phosphomolybdenum assays, having a dose-dependent effect. In the maximum absorbance test, the crude extract and its fractions could be used in a possible composition in formulations of a sunscreen, since antioxidants were found in large quantities and would act as a complement to sunscreens. The results allow concluding a possible sustainable use of E. edulis leaves due to their allelopathic potential their antioxidant activity and sun protection factors.
Avaliação da atividade alelopática, antioxidante, fotoprotetora e do perfil fitoquímico de extratos de folhas de Geonoma schottiana (Arecaceae)
(Universidade Federal do Espírito Santo, 2020-03-20) Gomes, Alessandro Bermudes; Corte, Viviana Borges; https://orcid.org/0000000254886578; http://lattes.cnpq.br/3694434981958328; https://orcid.org/0000-0003-0627-4996; http://lattes.cnpq.br/4784366298051203; Milanez, Camilla Rozindo Dias; https://orcid.org/0000-0002-9516-2031; http://lattes.cnpq.br/1288447995978779; Werner, Elias Terra; https://orcid.org/0000-0001-7781-4342; http://lattes.cnpq.br/3268068963656927; França, Hildegardo Seibert; https://orcid.org/0000-0001-6129-8793; http://lattes.cnpq.br/1284874997224988; Colman, Carine Coneglian de Farias; https://orcid.org/0000-0003-2967-3420; http://lattes.cnpq.br/9011970705908248; Seibert, Janaina Brandao; https://orcid.org/0000-0002-6112-5574; http://lattes.cnpq.br/5564487665806327
Geonoma schottiana Mart. is a native plant of the Atlantic Forest and it shows few studies on its biological and phytochemical potential. Therefore, this study aimed at bioprospecting of the G. schottiana leaves, evaluating its allelopathic, antioxidant, photoprotective potential and the phytochemical constitution of the ethanolic extract and its fractions (hexane, dichloromethane, ethyl acetate and butanol). The allelopathic activity was developed through biological tests with analysis of the germination variables and the initial growth, in which the hexane and butanol fractions showed the best results, as they potentiated the germination and growth variables on the lettuce and sensitized the colonio grass. The analysis of the hexane and butanol fractions at a concentration of 1.0 mg / mL promoted an increase in the activity of the antioxidant enzymes Catalase (CAT), Peroxidase (POX) and superoxide dismutase (SOD)as well as lipid peroxidation (MDA) on seed and roots of colonio grass. The contents of phenolic compounds and total flavonoids were evaluated by the Folin-Ciocalteau and aluminum chloride methods, respectively, while the tannins were determined by the casein precipitation method. The antioxidant activity was determined by ABTS, DPPH, FRAP, TAC-Phosphomolybdenum assay. The analysis of ABTS redox activity increased in the butanol fraction, as well as in the DPPH in the ethyl acetate and dichloromethane fractions and in the FRAP for the dichloromethane fraction, compared to the trolox equivalent (µMol Trolox / g of the extract). The TAC-Phosphomolybdenum assay was significant for the hexane and dichloromethane fractions compared to ascorbic acid equivalents (µg EAA g-1). Phytochemical prospecting was performed by thin layer chromatography (CCD), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (CG/EM). steroids, lignin, mono and diterpenes, alkali and flavonoids were identified in phytochemical prospecting by CCD for the hexane and butanol fractions. The HPLC analysis demonstrated the presence of chlorogenic acid in the crude extract and hyperoside in the ethyl acetate fraction, with the patterns isoquecertine, mirecithin and rutin found in the ethyl acetate and butanol fraction. The CG/EM of the hexane and dichloromethane fractions demonstrated the presence of phytol, a precursor to vitamin E. The photoprotection factor of the ethanolic extract and its fractions was evaluated, and the hexane fraction obtained the best result for the sun protection factor (6.39). In summary, the results obtained in this work indicate that the ethanol extract from G. schottiana leaves and its fractions have potential as a source of compounds with allelopathic, antioxidant and photoprotective activities.
Avaliação da resistência à antracnose em frutos de diferentes genótipos de bananeira
(Universidade Federal do Espírito Santo, 2005-02-28) Alves, Eliomara Sousa Sobral; Fernandes, Patricia Machado Bueno; Ventura, José Aires; Costa, Hélcio; Chiaradia, Ana Cristina Nascimento
In Brazil, the banana culture has a great economical importance. It usually has a family character in Espírito Santo state, being produced in small farms. However, both pre-and post-harvest diseases have caused severe crop-lost. Yellow and black-sigatoka and panama disease can affect the plants still in the field. After the harvest, the fruits can be infected by the Colletotrichum musaeresponsible for the anthracnose in bananas. The choice of resistant genotypes for those diseases is a viable strategy; they can be achieved through improvement programs. In this work the resistance of different bananas genotypes that showed pre-harvested resistance (‘Prata’; ‘Pacovan’; ‘Ouro da Mata’; ‘Prata Zulu’; FHIA01; PV 42-68; PV 42-81; PV 42-142; ST 12-31; ST 42-08; YB 42-21), against the anthracnose were evaluated, using the ‘Prata’ cultivar as control. Additionally, chemical and physical features of the resistant fruits physiology were determined. For the resistance analysis and the genotypes characterization it was used ten and three repetitions, respectively. Statistical variance was measured by 5 % Tukey test. Four hybrids (ST 12-31; PV 42-68; PV 42-81; PV 42-142) were more resist to disease confirmed by small lesion diameter and pulp preserved. ST 12-31, PV42-81 and PV 42-142 had higher weight values than control, being 150,7g, 187,8g and 243,5g, respectively. The values for pulp weight vary from 72,4g (control) to 138,5g (PV 42-142) and the peel/pulp ratio from 1,6 (control) to 3,9 (‘Prata Zulu’). The fruit size varies from 2,6 cm (control) to 3,59 cm (‘Prata Zulu’) in diameter and 13,6 cm (control) to 19,95 cm (PV 42-142) in length. The peel thickness values varied from 1,7 mm (‘Prata Zulu’) to 4,6 mm (PV42-142), different of control (3,0 mm). The biochemical analysis showed perceptual values 0,36, 0,69 and 0,63 for total titulable acids (ATT) presented in PV 42-68, ‘Ouro da Mata’ and control, respectively. The genotypes YB 42-21, ST 42-08 and ‘Prata’ had 4,53, 4,52 and 4,28 pH values, respectively. The total soluble solids (SST) vary from 24,8 % (control) to 27,4 % (‘Prata Zulu’) and the ATT/SST ratio vary from 38,72 (control) to 67,56 (‘Prata Zulu’). For starch meaning, we did not found variation among ‘Pacovan’, PV 42-68 and ‘Ouro da Mata’, although all of them had highest values than control. Among the evaluated genotypes, PV 42-142, PV 42-68, PV 42-81 and ST 12-31 self showed more attractive to be used for agriculture practice and they can be recommended for the small farms. The results presented are the primer steps for future improvement programs

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