Doutorado em Engenharia Ambiental

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Navegando Doutorado em Engenharia Ambiental por Assunto "Água"

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    Avaliação da qualidade de águas associadas a sistemas hemodialíticos
    (Universidade Federal do Espírito Santo, 2020-02-12) Gomes, Yohanna Mayelle; Cassini, Servio Tulio Alves; https://orcid.org/0000-0001-5200-3666; http://lattes.cnpq.br/2519874649699294; https://orcid.org/0000-0002-3045-9694; http://lattes.cnpq.br/0187262358266943; Goncalves, Ricardo Franci; https://orcid.org/0000000220489451; http://lattes.cnpq.br/9604327349698525; Mendonca, Antonio Sergio Ferreira; https://orcid.org/0000-0003-4273-0266; http://lattes.cnpq.br/9841888526169409; Santos, Kenia Valeria dos; https://orcid.org/0000-0001-6871-3128; http://lattes.cnpq.br/9074173162086323; Schuenck, Ricardo Pinto; https://orcid.org/0000-0001-9825-5762; http://lattes.cnpq.br/1211608551542058
    The objective of the research was to characterize the water quality for hemodialysis and to describe microorganisms present in the water. Ion chromatography methodologies were used to determine nutrient concentration in water, Elisa for microcystins detection, MALD-TOF MS for identification of isolated microorganisms, antibiogram test discs and minimum inhibitory concentration, action of disinfectants (UV, hypochlorite of sodium and peracetic acid) and metagenomic analysis to characterize the bacterial community present in the water treatment system. It was identified that the levels of nitrate, fluoride and BHT found may present a risk to the patient on hemodialysis. In a drinking water sample the presence of microcystin was verified. Of the isolates, Ralstonia sp. was the most frequent. About 60% of the isolated bacteria showed a pattern of multidrug resistance to antimicrobials, mainly colistin, gentamicin and carbapenemics. The water treatment system was able to remove 95% of the gene sequences detected in the incoming water. The most prevalent species were Sphingomonas alpina, Curvibacter gracilis, Bradyrhizobium sp., Ralstonia picketti, Escherichia coli, Serratia liquefaciens and Serratia proteamaculans. The presence of emerging pathogenic bacteria and multiresistance profiles detected in this study indicate the need for microbiological monitoring of water for hemodialysis