Caracterização de isolados de escherichia coli resistentes a antimicrobianos clinicamente importantes em bivalves marinhos nas cidades de Vitória e Vila Velha, Espírito Santo
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Data
2025-12-16
Autores
Gaigher , Gustavo Venturin
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Universidade Federal do Espírito Santo
Resumo
Antimicrobial resistance in coastal ecosystems represents an increasingly environmental and public health concern, and Escherichia coli is the predominant species in this scenario in Brazil. So, this study characterised E. coli isolates resistant to clinically relevant antimicrobials recovered from wild-harvested marine bivalves from Southeast Brazil, along a one-year surveillance study. Sampling was carried out at ten different sites at the Atlantic coast of Espírito Santo State. E. coli isolates obtained from selective media were identified by MALDI-TOF, evaluated for antimicrobial resistance profiles by phenotypic and molecular methods, and had phylogroups and clonal profiles evaluated by Clermont method and ERIC-PCR, respectively. A total of 151 isolates were recovered, of which most (90.73%) showed MAR index values > 0.2. ESBL production was predominant (93.38%), mainly codified by blaCTX-M-like genes. AmpC-producing isolates, carrying blaCMY-like; carbapenem-resistant strains, harboring blaKPC-2 or blaNDM-1; fluoroquinolones-resistant isolates, carrying qnrA, qnrB, qnrS or aac(6′)-Ib; and mcr-1-positive isolates, were also identified. Phylogrouping analysis revealed a predominance of commensal groups (E, A, and B1), while virulenceassociated phylogroups were less frequent. ERIC-PCR analysis revealed clonal diversity, with 19 clusters, and evidenced both long-term stability and geographic dissemination. Besides, the detection of different antimicrobial resistance genes in isolates from identical fingerprint patterns suggests horizontal gene transfer. Finally, occurrence of high-risk antimicrobial resistance determinants in marine bivalves poses potential threat to environmental and food safety, as well as public health, reinforcing the urgency of integrated surveillance strategies within a One Health approach.
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Moluscos , Microbiologia marinha , Testes de sensibilidade bacteriana