Fluorescência da clorofila a como ferramenta indicadora de estresse mineral em Schinus terebinthifolia Raddi
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Data
2025-03-31
Autores
Dalla, Rhaira Rodrigues
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Universidade Federal do Espírito Santo
Resumo
The aim of this study was to evaluate the effectiveness of chlorophyll a fluorescence as a diagnostic tool to identify and monitor abiotic stress in Schinus terebinthifolia Raddi, which occurs due to nutrient deficiency or excess, affecting its growth and metabolism. The deficiency reduces photosynthesis and development, while excess can cause toxicity. This results in chlorosis, necrosis and reduced resistance to pathogens, compromising its adaptation and regeneration. In addition, we sought to correlate changes in fluorescence indices and the modulation of proteins involved in the protection and adaptation of the photosynthetic apparatus. In vivo analyses of chlorophyll a fluorescence were performed using a portable fluorometer at four stations located in Espírito Santo (AE1, AE2, AE3 and AE4) in plants under abiotic stress conditions. These analyses allowed obtaining parameters such as maximum photosynthetic efficiency (Fv/Fm) and non-photosynthetic energy dissipation (NPQ), evaluated by OJIIP and SPAD. In addition, a search was conducted at NCBI for potential proteins to be used in the development of diagnostic tests in plants under mineral stress. The results of the study demonstrated that the exposure of Schinus terebinthifolia Raddi to different abiotic stress conditions caused marked changes in photosynthetic efficiency and a significant reduction in maximum photosynthetic efficiency (Fv/Fm) indices, indicating damage to the photosynthetic system resulting from the applied stresses. The increase in energy dissipation via NPQ evidences functional changes in the photosynthetic system that occur early in the face of mineral stress conditions suffered by the red pepper plant. Furthermore, five proteins, OHP1, OHP2, PSBS, ELIP1 and cpSRP43, were identified as potential proteins to be tested in a greenhouse using Arabidopsis thaliana as a model organism. Thus, the study showed that chlorophyll a fluorescence is a promising tool for the diagnosis of abiotic stress in Schinus terebinthifolia Raddi, allowing the early detection of imbalances in the photosynthetic apparatus. Furthermore, the proteins mentioned can be applied in later studies because they are associated with the assembly of the photosynthetic apparatus and interaction with pigments for the synthesis of chlorophyll
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Palavras-chave
Estresse abiótico , Eficiência fotossintética , NPQ , Proteínas fotossintéticas , Abiotic stress , Photosynthetic efficiency , Photosynthetic proteins