Atividade antimicrobiana de fenóis naturais contra microrganismos isolados do trato genital de éguas
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Data
2022-01-11
Autores
Lorenzoni, Priscila de Oliveira
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Universidade Federal do Espírito Santo
Resumo
Endometritis is one of the main causes of infertility in mares, generating economic losses in the equine reproduction sector. The difficulty in treating this disease is related to the emergence of resistant strains and the limited range of drugs available for specific use in the genital tract of mares. The need for a multifaceted medicine points to the discovery of new active ingredients. Thus, the study focuses on the study of antibacterial and antifungal properties of thymol and carvacrol, whose objective was to analyze the antimicrobial activity of these natural phenols on microorganisms isolated from the uterus of mares. In the experiment, uterine samples from embryo recipient mares (n=38) were collected for microbiological analysis and endometrial cytology. Isolated microorganisms were identified by DNA sequencing and the sensitivity of the microorganisms Escherichia coli, Staphylococcus sciuri and Rhodulotora glutinis to thymol or carvacrol was evaluated by microdilution assays in broth.In the results, it was possible to infer that 55.26% (n=21) of the animals had samples with microbiological growth, that is, they had endometritis. DNA sequencing identified 12 distinct species, 80% of which were bacteria and 20% fungi or yeasts. The most frequent microorganism was E. coli (50%). Timol and carvacrol showed bactericidal effect against E. coli, S. sciuri and fungicidal effect against R. glutinis isolated from the uterus of mares. The mean inhibitory concentration values (IC50) for thymol / carvacrol were, respectively, 0.101 / 0.076 mg/mL for E. coli, 0.192 / 0.197 mg/mL for S. Sciuri and 0.0378 / 0.0331 mg/mL for R. glutinis. The results observed in this study emphasize the potential of these natural phenols as an alternative therapeutic approach against uterine isolates resistant to other drugs or formulations available for the treatment of endometritis.
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carvacrol , endometrite , sequenciamento de DNA , timol