O papel da L-arginina sobre o sistema oxidativo em células mda-mb-231 de câncer de mama triplo negativo
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Data
2025-03-18
Autores
João Augusto Diniz Moura
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Universidade Federal do Espírito Santo
Resumo
L-Arginine is a semi-essential amino acid whose role in cancer has been under investigation, showing beneficial results due to its involvement in various physiological systems. Therefore, it is necessary to investigate which pathways involving this amino acid directly contribute to tumor regression, such as those related to oxidative stress. In this context, the use of the MDA-MB-231 triple-negative breast cancer cell line, known for its high aggressiveness and limited treatment options due to the lack of therapeutic targets, highlighted the effects of different concentrations of L-ARG on cell viability and the oxidative system. The treatment involved exposure to L-ARG at concentrations of 800 µg/mL, 1600 µg/mL, and 3200 µg/mL for 48 hours. A dihydroethidium (DHE) staining assay was performed to detect the increased presence of superoxide anion (O₂⁻). The AlamarBlue assay was employed to measure cell proliferation and assess cell viability. AOPP values were determined according to the protocol described by Witko-Sarsat et al. (1996) to evaluate the production of advanced oxidation protein products. Western blotting was used to determine the influence of L-arginine treatment on SOD1 protein expression in MDA-MB-231 cells across the NT, 800 µg/mL, 1600 µg/mL, and 3200 µg/mL groups. Data were analyzed using GraphPad Prism® version 8.0.2 and corrected by Tukey’s test. The main findings of the study were: (I) Reduced cell viability; (II) Increased production of superoxide anion radicals (O₂⁻) at all concentrations; (III) Elevated levels of advanced oxidation protein products; and (IV) Reduced SOD1 expression. Therefore, through the significant results that contributed to elucidating the effects of L-arginine on the oxidative system in MDA-MB-231 cells, this study opens new perspectives with potential translational implications.
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Câncer de mama , Estresse oxidativo , MDA-MB-231