Efeito modulador da laser terapia sistêmica na inflamação em modelo de acidente vascular encefálico
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Data
2024-10-18
Autores
Araújo, Stephanie Oliveira de
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Universidade Federal do Espírito Santo
Resumo
This master's dissertation analyzed the impacts of systemic photobiomodulation, through the non-invasive technique of Intravascular Laser Irradiation of Blood (ILIB), in an experimental model of global cerebral ischemia in mice. The main focus was to examine how this therapy acts in a dose-dependent manner in the recovery of brain injuries caused by bicarotid occlusion. The mice were exposed to this occlusion, after which ILIB was applied at a dose of 3.2 J (TL group) and 6 J (TH group), and the therapeutic effects were measured through functional and biochemical parameters. Motor evaluations of the mice were performed at two different times: before and after the induction of stroke, showing a significant improvement in the groups treated with ILIB, especially at the higher doses, improving the motor ability, strength and mobility of the mice, as well as a decrease in inactivity time. The macroscopic area of cerebral infarction was analyzed, and the treated group showed a reduction (p<0.01) in the area damaged by ischemia. In addition, blood samples were collected for analysis of blood cell counts, revealing a modulation in the inflammatory response in the groups that received ILIB (p<0.05). Plasma and brain were also extracted to measure oxidative stress (OS) levels and inflammatory markers, and in the TL and TH groups there was a modulation in the production of interleukins (IL-12p70, IL-6, IL-10, MCP-1 and IFN-γ); among these, there was a significant increase in anti-inflammatory interleukin and others that are responsible for the response to post-stroke infections; these effects were dose-dependent. A decrease in OS was observed in the TH group compared to the AVC group in the analysis of protein oxidation in plasma of 37% and in the brain of 35%, however, in lipid peroxidation it was 70% in plasma and 37% in the brain. It is noteworthy that ILIB in this model showed surprising results. Clinical studies should be carried out to standardize the dose in humans and the number of applications, which prove the effect, as well as clarify the specific mechanisms that allow ILIB to exert its neuroprotective effects
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Laser baixa intensidade , Acidente Vascular Encefálico , Inflamação , Estresse oxidativo , Fotobiomodulação sistêmica