DNA-barcode na identificação de espécies do complexo Hermanella (Ephemeroptera: Leptophlebiidae) e relações filogenéticas do grupo baseadas em multi-genes
Nenhuma Miniatura disponível
Data
2015-03-20
Autores
Gómez, María Paula Rozo
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal do Espírito Santo
Resumo
The generic complex Hermanella is a conspicuous group among the leptophlebiid mayflies and currently comprises eight genera, in spite of being a well accepted monophyletic group, the relationship between its members, the correct classification of some species and even the supra-specific status of some taxa is subject to debate. This study proposes to apply a DNA barcode to delimitate species of Hermanella complex using the intra-specific mean divergence value, in addition, confirming such data by using two methods: Poisson Tree Process (PTP) and the General Mixed Yule-Coalescent (GMYC) and infer the phylogenetic relationships between the taxa by using molecular phylogenetic reconstruction with nuclear and mitochondrial markers to test the effectiveness of species delimitation model by DNA barcode. In order to do so, 13 species of the Hermanella complex were identified and three from the Homothraulus complex and two mtDNA fragments: Cytochrome Oxidase I (COI) and Cytochrome Oxidase II (COII); as well as two nuclear genes: Histone 3 (HEX) and the 28S-D1 region. Analysis of genetic divergence, PTP and GYMC were carried out to delimit species based on the COI gene (DNA barcode) as well as Maximum Likelihood (MV) and Bayesian Inference (IB) for each gene both independently and concatenated. Analysis of species delimitation with DNA barcode agreed to validate 7 out of the 13 original species as phylogenetic species: Hd. plagatus, Hd. primanus, Hd. gilliesae, Hm. sallesi, Hy. obliquus, Needhamella ehrhardti e T. insolita. Similarly, agreed to split as different phylogenetic species the populations of Hy. plaumanni haplótipo 23, Hy. plaumanni haplótipo 24, L. palpalis AP, L. palpalis ES, L. palpalis RO, L. palpalis RR, Needhamella sp. nov GO, Needhamella sp. nov PE, P. convexa AP, P. convexa BA, P. convexa RO e P. convexa RR. The monophyle of the Hermanelle complex was confirmed by both topopogies MV and BI, besides to recover Hermanella as sister group to Hylister and Hydrosmilodon as paraphyletic group. Needhamella related to Paramaka, a Hd. gilliesae related to Leentvaaria and Hd. primanus as sister group of Hd. gilliesae. All phylogenetic species delimited through DNA barcode were grouped as well supported clades with IB hypothesis and reasonably supported with MV. This agreement between the results of the analysis is a clear evidence of the DNA - barcode was and effective method to delimit species in the Hermanella complex and the molecular markers were successful in recovering phylogenetic hypotheses that complement and support the results, however it is important to remember that morphological studies, ecological and biogeographic are needed to deepen the information obtained in this study.
Descrição
Palavras-chave
Phylogeny , Species delimitation , Sistemática filogenética , Inferência bayesiana , Bayesian inferense , Maximum likelihood
Citação
GÓMEZ, María Paula Rozo. DNA barcode na identificação de espécies do complexo Hermanella ( Ephemeroptera: Leptophlebiidae) e relações filogenéticas do grupo baseadas em multi-genes. 2015. 64 f. Dissertação (Mestrado em Biodiversidade Tropical) - Universidade Federal do Espírito Santo, Centro Universitário Norte do Espírito Santo, São Mateus, 2015.