Identificação de alterações bioquímicas no soro de indivíduos submetidos à gastroplastia redutora em Y de Roux por espectroscopia no infravermelho médio
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Data
2024-06-11
Autores
Bortoli, Amanda Motta de
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Universidade Federal do Espírito Santo
Resumo
Bariatric surgery, especially Roux-en-Y gastric bypass (RYGB), is the most indicated treatment for severe obesity, promoting significant biochemical alterations. Fourier transform infrared vibrational spectroscopy (FTIR) has been applied in biological analyses with the potential to detect such changes. This study investigated the use of FTIR for identifying biochemical alterations in the serum of individuals undergoing RYGB. An observational and descriptive study was conducted with patients from the Bariatric and Metabolic Surgery Program of a university hospital, before (T0) and approximately two months after RYGB (T1). Biochemical parameters, anthropometric data, and body composition were evaluated. FTIR spectra were recorded using ALPHA II equipment (Bruker, Germany), pre-processed, and subjected to Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA) (MATLAB 2023b). Data normality was tested using the Kolmogorov-Smirnov test, followed by paired T-test or Wilcoxon test. Spearman correlation analysis of spectral information with biochemical parameters was performed. A significance level of p <0.05 was adopted for all tests (GraphPad Prism 8 software). Twenty-nine individuals (86.2% female) with a mean age of 41.2 ± 7.8 years were evaluated. Anthropometric and body composition variables significantly decreased (p <0.001). Serum concentrations of albumin, transthyretin, C-reactive protein, alkaline phosphatase, glucose, triglycerides, total cholesterol, and LDL cholesterol also showed significant reductions (p <0.05). PCA demonstrated a trend of separation at T0 and T1 generated by biochemical alterations. Different significant correlations between biochemical parameters and spectral regions were identified at T0 and T1; however, only serum triglyceride concentration at both time points (p <0.05) correlated significantly with the spectral region characteristic of lipids (1796 - 1685 cm-1 ). The results suggest that FTIR exhibits sensitivity to identify alterations related to the lipid profile of individuals undergoing surgical treatment for severe obesity.
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Cirurgia Bariátrica , Perfil lipídico , FTIR