Mestrado em Ciências Odontológicas
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- ItemAnálise de alterações nucleares nas mucosas jugal, labial e lingual em indivíduos com epidermólise bolhosa residentes no Espírito Santo e correlações clínico-patológicas(Universidade Federal do Espírito Santo, 2025-02-27) Badaró, Rebeca Ferreira; Souza, Letícia Nogueira da Gama de; https://orcid.org/0000-0001-6330-996X; http://lattes.cnpq.br/2797885632539769; Freitas, Vanessa Morais; Barroso, Danielle Resende CamisascaEpidermolysis Bullosa (EB) is a severe genetic disorder characterized by the continuous formation of blisters on the skin and mucous membranes, potentially leading to significant structural alterations in the oral cavity. This study aimed to analyze nuclear aspects of cytotoxicity and mutagenicity in exfoliated cells from the buccal, labial, and lingual mucosa of patients with EB. Sociodemographic and clinical data were collected through physical examination and a standardized questionnaire. Exfoliated cells from the buccal, labial, and lingual mucosa were assessed for the frequency of micronuclei (MN), karyorrhexis (KR), karyolysis (KL), and pyknosis (PN). The Mann-Whitney and Kruskal-Wallis tests were used for statistical analysis. The study included 20 EB patients and 20 age- and sex-matched controls. A similar distribution was observed between males (40%) and females (60%), with a predominance of individuals ≤19 years old (65%). Quantitative analysis revealed a significant increase in KR and KL in EB patients compared to controls, particularly in the buccal (p=0.0002; p<0.0001) and labial mucosa (p=0.0005; p=0.0240). Additionally, MN frequency was higher in the EB group, especially in the buccal mucosa (p=0.0083). These findings indicate an increase in cytotoxic and mutagenic events in the oral mucosa of EB patients, suggesting a link between genomic instability and the disease. Cytological smear analysis may serve as a promising tool for the dental monitoring of these patients, particularly in severe cases
- ItemAvaliação do impacto da saúde bucal na qualidade de vida de uma população rural pomerana(Universidade Federal do Espírito Santo, 2025-03-31) Maria, Lorrayne Cesario; Miotto, Maria Helena Monteiro de Barros; https://orcid.org/0000-0002-3227-7608; Sanglard, Luciana Faria; Barcellos, Ludmilla AwadStudies dedicated to assessing the quality of life of the rural population and the community are scarce. Expanding research on Pomeranians is essential in order to contribute to the development of public policies that meet their specific needs. This study assessed the impact of oral health on quality of life, possible associations with sociodemographic variables, demand for dentures, dental status, type of access and reason for using dental services in a rural Pomeranian population. This is a cross-sectional study carried out with 300 descendants of Pomeranians, preferably of direct descent, who live in the district of Melgaço, municipality of Domingos Martins, state of Espírito Santo. Data was collected through face-to-face interviews at home, using the Oral Health Impact Profile - 14 instrument to assess quality of life and a structured questionnaire with the other study variables. The Fischer Exact and Mantel Haenszel tests were used for statistical analysis, and the odds ratio was calculated. The impact of oral health on quality of life was declared by 68 individuals (22.7%), with a higher prevalence in the following dimensions: psychological discomfort (13%), physical pain (12.3%) and psychological disability (12%). This impact was associated with individuals aged 41 or over, who had studied up to 12 years, who were non-white and who used the public health service or had no access to it. The absence of at least one tooth, the demand for dental prostheses and the use of dental services as a matter of urgency were factors that had an impact on the quality of life of 22.7% of these individuals.
- ItemAtitude dos professores da rede municipal de ensino frente à situação de traumatismo dentário(Universidade Federal do Espírito Santo, 2024-02-08) Santos, Pamela Barbosa dos; Miotto, Maria Helena Monteiro de Barros ; https://orcid.org/0000-0002-3227-7608; http://lattes.cnpq.br/4289442514763843; https://orcid.org/0000-0002-4155-7441; http://lattes.cnpq.br/8951452929618117; Sanglard, Luciana Faria ; https://orcid.org/0000-0002-2520-7062; http://lattes.cnpq.br/4718823259148176; Barcellos, Ludmilla Awad ; https://orcid.org/0000-0003-3732-9515; http://lattes.cnpq.br/7262427405068645Objective: To evaluate the attitudes of teachers from the municipal education network of the Vitória City Hall in different dental trauma scenarios and associate them with socioeconomic, demographic, and functional variables. Material and Methods: A quantitative, cross-sectional, and probabilistic study was conducted. Data collection was carried out through self administered and validated questionnaires, addressing sociodemographic and functional information as well as knowledge about dental trauma. Correct or incorrect attitudes were assessed in three different scenarios. Statistical analyses included chi-square tests (p < 0.05), descriptive analyses, and frequency tables with numbers and percentages. A total of 292 teachers from 37 schools participated in the study. Results: Among the participants, 137 (46.9%) teachers had witnessed cases of dental trauma, with 94 (68.6%) of these cases occurring in the school environment. Additionally, 274 (93.8%) teachers had never received formal guidance on the subject. It was observed that teachers who received guidance on dental trauma in scenario 3 had a statistically significant association (p = 0.030) with a higher frequency of correct attitudes among those who had undergone training on the topic. Similarly, teachers who had experienced dental trauma situations showed a potentially significant association (p = 0.056) with correct attitudes. No statistically significant differences were observed concerning socioeconomic and sociodemographic variables. Conclusion: The scenario assessment revealed a significant prevalence of incorrect attitudes regarding dental avulsion cases among teachers. This underscores the urgent need to implement educational actions and training programs, as well as to include the topic in teacher education, aiming to prepare them to effectively handle such situations.
- ItemEfeitos da terapia a laser de baixa intensidade sobre as células da gengiva de camundongos com periodontite experimental(Universidade Federal do Espírito Santo, 2024-07-15) Braga, Larissa Trarbach Figueiredo; Porto, Marcella Leite; Meyrelles, Silvana dos Santos; https://orcid.org/0000-0003-0167-4093; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; Campagnaro, Bianca Prandi; https://orcid.org/; http://lattes.cnpq.br/; Rosetti, Elizabeth Pimentel; https://orcid.org/; http://lattes.cnpq.br/Introduction: Periodontitis is a multifactorial chronic inflammatory disease associated with dysbiotic microorganisms that, through the presence of bacterial biofilm, alter the homeostatic environment of the periodontium and impair systemic health. The inflammatory changes hinder the host's immune response and cause degradation of the protective and supportive tissues of the teeth, which can consequently lead to tooth loss. Low-Level Laser Therapy (LLLT) has been studied for its potential to reduce inflammation and influence cellular processes with positive results. However, its use in conjunction with periodontal treatments remains debatable. Objectives: To evaluate the effect of LLLT on reactive oxygen species (ROS) and apoptosis in gingival tissue, and on systemic parameters related to inflammation and oxidative stress caused by experimental periodontitis. Methods: This experimental research was conducted on 16-week-old C57/BL6 mice, randomly divided into four groups: control (C) - no intervention; control+LLLT (C+L) - no intervention and treated with LLLT; periodontitis (P) - with ligature-induced periodontitis; periodontitis+LLLT (P+L) - with ligature-induced periodontitis and treated with LLLT. After 28 days of induction, the ligature was removed and the animals were treated with scaling and root planing (SRP), and LLLT was applied for 3 days (4 joules per session). At the end of the treatment, the animals were euthanized; the gingiva, mandible, and plasma were separated for analysis. In the mandible, to confirm periodontitis, we measured alveolar bone loss using scanning electron microscopy (SEM). In gingival tissue, ROS levels and cell apoptosis were evaluated by flow cytometry. In plasma, inflammatory activity was analyzed through the enzyme myeloperoxidase (MPO), and protein oxidation was assessed using the advanced oxidation protein products (AOPP) assay, both by spectrophotometry; cytokine levels were measured using flow cytometry. Results: Periodontitis was confirmed. LLLT reduced superoxide anion and hydrogen peroxide levels in gingival tissue by approximately 18% and 26%, respectively. Apoptosis in gingival cells was 32% lower in LLLT-treated animals with periodontitis. Systemic inflammatory activity and protein oxidation decreased by 49.3% and 38.6%, respectively. In the P+L group, plasma levels of pro-inflammatory cytokines IL-6 and IL-12p70 were reduced, while the anti-inflammatory cytokine IL-10 increased compared to the P group (p<0.05). Conclusion: In this experimental model, the use of LLLT effectively regulated ROS levels and apoptosis in gingival tissue, influencing plasma markers of oxidative stress and inflammation.
- ItemEpidermólise bolhosa no Espírito Santo: caracterização sociodemográfica e avaliação de alterações na mucosa oral(Universidade Federal do Espírito Santo, 2024-07-26) Franzosi, Ana Carolina Sias Franco; Souza, Letícia Nogueira da Gama de; https://orcid.org/0000-0001-6330-996X; http://lattes.cnpq.br/2797885632539769; https://orcid.org/0009-0000-8665-4993; http://lattes.cnpq.br/6128025364510979; Coburn, Karla Loureiro Almeida; https://orcid.org/0000-0001-8424-1394; http://lattes.cnpq.br/1128536176975528; Errera, Flavia Imbroisi Valle ; https://orcid.org/0000-0002-8069-6372; http://lattes.cnpq.br/9337327437538048Introduction: Epidermolysis Bullosa (EB) is a severe genetic disease characterized by continuous blistering of the skin and mucous membranes, potentially presenting significant structural changes in the mouth. Objective: To analyze the nuclear aspects of cytotoxicity and mutagenicity obtained from exfoliated cells of the buccal mucosa in EB patients. Materials and Methods: Sociodemographic and clinical information were collected through physical examination and a standardized questionnaire. Buccal mucosa cells were obtained from oral exfoliations and evaluated to determine the frequency of micronuclei, karyorrhexis, karyolysis, and pyknosis. The statistical tests used were Mann-Whitney and Kruskal-Wallis. Results: 20 EB individuals were selected, and the control group (n=20) was matched for sex and age with the EB sample. A similar distribution was observed between men (n=8, 40%) and women (n=12, 60%), and individuals aged ≥ 19 years represented the majority (n=13, 65%). Quantitative analysis revealed that most cells were identified as normal in both groups. A higher number of karyorrhexis (p=0.0002) and karyolysis (p<0.0001) was observed in the EB sample. Micronuclei were found only in the latter group. When comparing non-EB patients with the most severe cases of the disease, a higher number of karyolysis was identified in the EB sample (p=0.0006). Conclusion: Nuclear alterations of cytotoxicity were more frequent in the EB group. Mutagenicity-related changes were found only in this group. This reinforces the need for constant medical and dental follow-up to prevent the establishment of more severe conditions.