Doutorado em Biologia Vegetal

URI Permanente para esta coleção

http://repositorio.ufes.br/handle/10/17505

Nível: Doutorado
Ano de início: 2013
Conceito atual na CAPES: 4
Ato normativo: Homologado pelo CNE (Portaria MEC Nº 609, de 14/03/2019. Publicação no DOU 18 de março de 2019, seç. 1, p.63 - Parecer CNE/CES nº 14/03/2019, Processo no 23001.000335/2018-51).
Periodicidade de seleção: Anual
Url do curso: https://biologiavegetal.ufes.br/pt-br/pos-graduacao/PPGBV/detalhes-do-curso?id=99

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    Diversidade genética de populações de Bidens pilosa e Tithonia diversifolia no Espírito Santo e respostas do metabolismo primário e secundário sob diferentes condições de fertilização e irrigação
    (Universidade Federal do Espírito Santo, 2018-03-22) Luz, Anny Carolyne da; Batitucci, Maria do Carmo Pimentel; Werner, Elias Terra; Ventura, Jose Aires; Vargas, Sarah Maria; Gonçalves, Rita de Cássia Ribeiro
    Bidens pilosa L. and Tithonia diversifolia (Hemsley) A. Gray are plants of the Asteraceae family with distribution in tropical and subtropical regions. They are plants used for various therapeutic purposes, mainly anti-inflammatory, antiseptic, hepratoprotective and prevention of cancer. The plants of this family present a complex chemical defense system, with derived secondary metabolites: phenolic compounds, flavonoids, serquiterpene lactones and polyacetylenes. These compounds have their production and accumulation modified in response to environmental, ontogenetic and also hereditary variations. Thus, it is important to control and standardize all phases involved in the production of phytopharmaceuticals, from the selection of the initial plant material, the conditions of cultivation, post-harvest management and extraction methods of the bioactive compounds to final product. The objectives of this study were to evaluate using RAPD molecular markers, the genetic diversity of different populations of B. pilosa and T. diversifolia. Also analyze how the management of the growth conditions can influence the growth, the photosynthetic parameters, the production of secondary metabolites, the antioxidant and cytotoxic activity of these plants. B. pilosa presented low levels of similarity, indicating that the grouping found is not only related to the geographic proximity, but probably to the dispersion of seeds through of animals and the contamination of species cultivated with B. pilosa seeds, which facilitated the gene flow, enabling the population of distant sites to be grouped in a closer cluster due to their common origin. In the analyzes of T. diversifolia high levels of genetic diversity were found, not positively correlated with the proximity of geographic locations. Although it is often propagated by cuttings, T. diversifolia reproduces sexually, with a large number of seeds and presents an abundant variety of pollinators insects, characteristics that contribute to a greater variability genetic. Regarding the management of the cultivation conditions, in general, the fertilizer applications increased the photosynthesis and the total dry mass for the two species studied. However, the plants that did not receive fertilization had the highest levels of phenolic compounds and higher antioxidant activity, a result related to the lower availability of nitrogen to no fertilized plants. These results indicate that there is an increase in the production of secondary metabolites when liquid photosynthesis decreases, resulting from the positive regulation of the shikimic acid in the pentose phosphate pathway. The species did not show cytotoxic activity in human lymphocytes while decreasing cell viability in sarcoma 180. It is important to standardize the management practices in Asteraceae cultivation in order to achieve high levels of phenolic compounds and biomass, linking the increasing of biological activity efficiency with increased productivity.