Doutorado em Ciências Fisiológicas

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Navegando Doutorado em Ciências Fisiológicas por Autor "Abreu, Glaucia Rodrigues de"

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    Caracterização dos efeitos da euphorbia tirucalli sobre a função cardíaca de ratos normotenso
    (Universidade Federal do Espírito Santo, 2023-02-15) Barroso, Maria Eduarda de Souza; Meyrelles, Silvana dos Santos; https://orcid.org/0000-0003-0167-4093; http://lattes.cnpq.br/7731215198101947; https://orcid.org/0000-0002-7696-0919; http://lattes.cnpq.br/7624081067739433; Abreu, Glaucia Rodrigues de; https://orcid.org/0009-0008-8772-8470; http://lattes.cnpq.br/0229590907405570; Baldo, Marcelo Perim; https://orcid.org/0000-0002-7673-3580; http://lattes.cnpq.br/7820422119282248; Pereira, Thiago de Melo Costa; https://orcid.org/0000-0003-4053-5061; http://lattes.cnpq.br/2027151219358701; Kuster, Ricardo Machado; https://orcid.org/0000000289615348; http://lattes.cnpq.br/4149814906786366
    There are many reports of people about using medicinal plants, ignore the existence of real scientifically proven effectiveness and many others end up abandoning allopathic treatments in the hope of healing through these plants. This behavior has become very common among patients diagnosed with cancer who, in search of a cure. The aveloz (Euphorbia tirucalli) it is a plant of African origin that is popularly used for various diseases, including the treatment of cancer. This latex has already presented several therapeutic actions such as antioxidant, immunomodulatory and even anticancer activity in some cell colonies. Most of the studies, however, were carried out in vitro, with a lack of in vivo studies to certify its properties. The aim of the present work was to characterize the actions of Euphorbia tirucalli on the cardiovascular system of wistar rats. For this, wistar rats were divided into 2 groups: control treated with water and treated with E. tirucalli at a dose of 13,06mg/Kg for 15 days by gavage. The animals were evaluated for blood pressure by tail plethysmography and functional aspects of the cardiovascular system such as contractility and relaxation were analyzed by cardiac hemodynamics. Tissue parameters such as cardiac remodeling and pulmonary congestion were also analyzed. The investigation of cellular parameters included the analysis of oxidative stress markers in the DNA of blood cells, bone marrow cells and heart cells, as well as biomolecular analyzes of oxidative stress in plasma. The percentage of apoptosis and possible necrosis was evaluated in cardiac cells from animals in both groups. The animals showed no difference between their weights and blood pressure before and after the treatments. Cardiac remodeling and pulmonary congestion indices did not differ significantly between groups. In the hemodynamic evaluation, the treated animals did not show changes in intraventricular pressure and heart rate, but a significant increase in the duration of the cardiac cycle can be observed in animals treated with E. tirucalli. In the evaluation of ventricular contractility (dp/dtmax + ) there was a significant increase in animals treated with E. tirucalli and a decrease in TAU, with no changes between groups in the dp/dtmax -. In the biochemical analyzes, a significant increase in AOPP can be observed (15,86±0,8µmol/L control group; 30,48±2,4µmol/L E. tirucalli group.) and Myeloperoxidade (0,051±0,006UA control group; 0,111±0,016UA E. tirucalli group) with this difference not being maintained between groups in the TBARS analysis. During the evaluation of reactive species in the isolated cells, an increase in the marking of DHE and DCF in the blood of the animals treated with speed 15 days can be observed, a result that is not repeated in the isolated cells of the bone marrow and only DCF demonstrated to be increased in cells heart of rats treated with E. tirucalli. No significant difference was demonstrated between the parameters and viability, apoptosis and necrosis evaluated in cardiac cells. The results indicate a possible harmful action of E. tirucalli on parameters of contractility and relaxation of the cardiac muscle and that this phenomenon probably involves oxidative stress. This implies the need for further investigations to better elucidate their mechanisms of action on a healthy organism.
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    Efeito protetor do ácido elágico sobre a reatividade vascular e parâmetros morfofuncionais da aorta de ratos expostos por trinta dias ao cloreto de cádmio
    (Universidade Federal do Espírito Santo, 2025-08-29) Simões, Rakel Passos; Vassallo, Dalton Valentim; https://orcid.org/0000-0002-4463-4174; http://lattes.cnpq.br/7749285591179880; Padilha, Alessandra Simão ; https://orcid.org/0000-0002-9585-1347; http://lattes.cnpq.br/7658998034219799; https://orcid.org/0000-0003-0027-8128; http://lattes.cnpq.br/1703421305699091; Nunes, Karolini Zuqui ; https://orcid.org/0000-0003-3433-4925; http://lattes.cnpq.br/6888896554912256; Batista, Priscila Rossi de; https://orcid.org/0000-0001-5850-0989; http://lattes.cnpq.br/9629149780640340; Pereira, Camila Almenara Cruz ; https://orcid.org/0000-0001-7889-4161; http://lattes.cnpq.br/3103606418826712; Abreu, Glaucia Rodrigues de; https://orcid.org/0009-0008-8772-8470; http://lattes.cnpq.br/0229590907405570
    Cadmium (Cd) is a toxic heavy metal widely present in the environment, and its chronic exposure is associated with several deleterious effects on the cardiovascular system, including arterial hypertension and endothelial dysfunction. Ellagic acid (EA), a natural polyphenol with antioxidant, anti inflammatory, and chelating properties, has been proposed as a potential vascular protective agent. This study investigated the effects of EA on cardiovascular alterations induced by subchronic exposure to CdCl₂ (100 ppm for 30 days) in Wistar rats. Animals were divided into four experimental groups: Control (Ct), Cd, EA, and Cd+EA. Cd was administered via drinking water, and EA was given by gavage (30 mg/kg/day). Systolic blood pressure (SBP) was monitored weekly using tail-cuff plethysmography. At the end of the protocol, blood Cd concentration was consistent with levels observed in human occupational exposure (31.15 ± 2.8 µg/L). To assess vascular function, thoracic aortic rings were mounted in organ baths and subjected to protocols with phenylephrine, acetylcholine (ACh), and sodium nitroprusside (SNP), with or without endothelium, and in the presence of inhibitors (L-NAME, apocynin, catalase, allopurinol, and SOD). Cd exposure reduced body weight, increased SBP, enhanced vascular reactivity to phenylephrine, and impaired endothelium dependent relaxation, effects associated with reduced nitric oxide (NO) bioavailability and increased reactive oxygen species (ROS) production. Increased collagen deposition and endothelial cell damage were also observed. EA treatment prevented these changes, restoring endothelial function, normalizing vascular tone, and preserving collagen content and endothelial cell integrity. These findings indicate that EA exerts a significant protective effect against Cd-induced vascular toxicity and appears promising as a functional agent for the prevention of cardiovascular diseases
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    Efeitos do ácido elágico sobre a reatividade vascular em artérias de resistentes de ratas hipertensas e ovariectomizadas
    (Universidade Federal do Espírito Santo, 2019-08-23) Silva, Fabricio Braganca da; Abreu, Glaucia Rodrigues de; https://orcid.org/0009-0008-8772-8470; http://lattes.cnpq.br/0229590907405570; https://orcid.org/; http://lattes.cnpq.br/3532755171099760; Mill, Jose Geraldo; https://orcid.org/000000020987368X; http://lattes.cnpq.br/2497419234600362; Mauad, Helder; https://orcid.org/0000-0001-9694-2593; http://lattes.cnpq.br/4554702077415995; Brasil, Girlandia Alexandre; https://orcid.org/0000-0002-5455-7141; http://lattes.cnpq.br/1402295792093274; Padilha, Alessandra Simao; http://lattes.cnpq.br/7658998034219799
    The postmenopausal period is considered a milestone for the emergence of chronic noncommunicable diseases, including cardiovascular diseases. In this context hypertension and endothelial dysfunction form the basis of the main risk factors and injuries. The introduction of nutraceuticals as therapeutic alternatives has attracted the attention of researchers for presenting promising results. These include ellagic acid, a phytochemical with potent antioxidant properties found in fruits, plants, and nuts. Therefore, the objective of this research was to evaluate the effects of ellagic acid treatment on vascular reactivity of resistance arteries in an experimental model of hypertension and menopause. Therefore 40 SHR rats were evaluated for 4 weeks and separated into 5 groups according to treatment: vehicle-treated SHAM (carboxymethylcellulose); OVX, castrated and vehicle treated; OAE, castrated and treated with ellagic acid (10 mg / kg / day); OE2, castrated and treated with estrogen (1g / kg / day); OAE + E2, treated with ellagic acid and estrogen. Mesenteric artery rings were maintained in vitro and subjected to contraction strength tests in the absence and presence of the nitric oxide synthase enzyme inhibitor to record the effect of treatments and the participation of nitric oxide (NO). Vessel samples were taken for biochemical analysis. Castration produced an increase in blood pressure (MAP: 190.4 ± 4.4 mmHg); effect that did not occur in groups treated with ellagic acid (MAP: 165.7 ± 1.4 mmHg, p
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    Efeitos dos diferentes tempos de início de treinamento físico após o infarto do miocárdio sobre a função cardíaca e o remodelamento ventricular esquerdo em ratas ovariectomizadas
    (Universidade Federal do Espírito Santo, 2017-05-12) Claudio, Erick Roberto Gonçalves; Abreu, Glaucia Rodrigues de; Leite, Richard Diego; Gouvêa, Sônia Alves; Futuro Neto, Henrique de Azevedo; Mauad, Helder
    The aim of the present study was to evaluate the effects of the different time of exercise training (ET) initiation after myocardial infarction (MI) on the cardiac function and the ventricular remodeling in ovariectomized rats (OVX). Twelve-week-old OVX female Wistar rats (Rattus Norvegicus Albinus) were randomly divided into four groups: MI-SHAM (SHAM), MI sedentary (MI), MI which started ET 3 days after MI (MI3+ET) and MI which started ET 15 days after MI (MI15+ET). Low to moderate intensity training protocol in a motorized treadmill was performed during 8 weeks (60 min/d, 5 days/wk). Forty-eight hours after the last training session, animals were anesthetized and subject to evaluation of the following parameters: i) Cardiac function after a catheter insertion in the left ventricle (LV); ii) MI area; iii) collagen deposition and myocyte hypertrophy by histology; iv) myocardial oxidative stress by the “in situ” generation of superoxide (DHE) and by the evaluation of the advanced oxidation protein products (AOPP); v) protein expression of type 1 receptor of angiotensin II (AT-1R) and of the membrane isoform of multi-enzymatic complex of NADPH oxidase (gp91phox) by the western blotting (WB) method; vi) antioxidants protein expression, such as superoxide dismutase (SOD-1 and SOD-2) and catalase (CAT), also by WB; vii) antioxidant enzymes activities (SOD and CAT).
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    Eficácia dos exercícios de alongamento e de estabilização lombar no tratamentro de lombalgia crônica
    (Universidade Federal do Espírito Santo, 2010-08-03) Maria Angelica Ferreira Leal Puppin; Marques, Amélia Pasqual; Futuro Neto, Henrique de Azevedo; Ferreira , Elizabeth Alves Gonçalves; Abreu, Glaucia Rodrigues de; Araújo , Maria Teresa Martins de
    Low back pain affects over 70% of the population, mostly economically active adults. In addition to the high incidence, chronicity and disability makes this disorder a public health problem in industrialized countries. Kinesitherapy is the first line of choice in physical therapy, but there is no evidence of which type of exercise is more effective. The aim of this study, was to assess, the effectiveness of the GDS method on pain, functional disability, global flexibility and ability to contract the transverse muscle of abdomen (TrA) in individuals with chronic low back pain. 82 patients were randomized into three groups: Stretching (n = 30, age 37.5 ± 12.1) subjected to stretching exercises; Stabilization (n = 27, age 39.0 ± 12) which performed exercises to recruit the deep muscles of the lower trunk and Control (n = 25, age 37.8 ± 13.6) that were not treated. The intervention groups were treated with two weekly sessions lasting 40 minutes, for eight weeks. Pain was assessed by visual analogue scale; functional disability by the Oswestry Index, global flexibility by the third finger to the ground and the ability of TrA contraction by the pressure biofeedback unit. The variables were analyzed before, after and after eight weeks of treatment. We used the single-factor ANOVA with repeated measures and post hoc Holm-Sidak for parametric variables and Friedman's test and Tukey for non parametric at comparisons within and between groups. A significance level of 5% was employed. We also evaluated the relative clinical gain. The results showed significant improvement in pain, functional disability and overall flexibility after treatment and after eight weeks (p <0.05) in the intervention groups. Only the stabilization group was effective in improving the capacity of contraction of TrA (p <0.05). When comparing groups, the two forms of intervention were effective in reducing pain and functional disability (p <0.05). Nevertheless only the Stretching group showed significant improvement in global flexibility (p = 0.01) There was no differences between groups after eight weeks of treatment in functional disability (p = 0.10) and global flexibility (p = 0.07). There was no difference between groups in the ability of contraction of TrA in any of the times evaluated. The two experimental groups had gains on clinical relevance, especially the Stabilization group, except for global flexibility variable in which the stretching exercises had higher gains. The two treatments, stretching and lumbar stabilization used in GDS were effective in reducing pain, functional disability and improving global flexibility, but only the stabilization exercises showed improved ability of the contraction of the TrA in patients with chronic low back pain.