Doutorado em Doenças Infecciosas

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http://repositorio.ufes.br/handle/10/17578

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Navegando Doutorado em Doenças Infecciosas por Autor "Braga, Fabio Ribeiro"

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    Alcoolismo e Strongyloides stercoralis : investigação de possíveis fatores associados à maior prevalência do nematoide em alcoolistas crônicos
    (Universidade Federal do Espírito Santo, 2017-02-17) Ribeiro, Steveen Rios; Pereira, Fausto Edmundo Lima; Falqueto, Aloísio; Gomes, Daniel Claudio de Oliveira; Braga, Fabio Ribeiro; Leite, Gustavo Rocha
    Introduction. Strongyloides stercoralis (Bavay, 1876) is an intestinal nematode that can proliferate in the host by autoinfection, and persist for decades without further exposure to exogenous infection. The prevalence of the parasite is poorly known in the state. Higher prevalence of S. stercoralis in chronic alcoholic patients than in non-alcoholics, attended at the same hospital, has been reported, but there are doubts about if there is a greater exposure to the parasite or greater survival and fecundity of females in the duodenum would increase the chance of encountering larvae in the feces. Objectives. To evaluate: (a) the prevalence of S. stercoralis in the state on samples of schoolchildren aged 7 to 14 years and samples from patients attended at the different outpatient clinics of the Hospital C.A. Moraes (HUCAM) and Hospital Concórdia at Sta. Maria de Jetibá; (b) the prevalence of the parasite on samples of alcoholics and non-alcoholics in the Department of Gastroenterology of HUCAM and Hospital Concordia; (c) the number of S. stercoralis rabditoid larvae on stools of alcoholics and non-alcoholics patients; (d) the anti-S. stercoralis antibodies on serum and Treg lymphocytes (Treg) in peripheral blood in alcoholics and non-alcoholics; (e) the use of the conventional PCR method on stool samples of alcoholic and non-alcoholic patients, to confirm the difference in prevalence observed between the two groups.
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    "Ocorrência de Ehrlichia spp., Anaplasma spp., Babesia spp., Hepatozoon spp. e Rickettsia spp. em cães domiciliados em seis municípios do Estado do Espírito Santo, Brasil"
    (Universidade Federal do Espírito Santo, 2017-07-20) Vieira, Fernanda de Toledo; Dietze, Reynaldo; Braga, Fabio Ribeiro; Moraes Filho, Jonas; Santos, Kênia Valéria dos; Palaci, Moises
    Tick-borne diseases are an important cause of morbidity and mortality in dogs (Canis lupus familiaris) worldwide, with the tick Rhipicephalus sanguineus sensu lato (s.l.) as a vector of various agents. It is very important to know the prevalence of these diseases, their vectors and hosts in order to control and prevent them in the given region. Tick-borne diseases have importance for both veterinary medicine and for public health. This work aims to identify and quantify the percentage of infection by Rickettsia spp., Ehrlichia spp., Babesia spp. and Hepatozoon spp. in pet dogs and identify ticks collected on these animals in the municipalities of Santa Teresa, Colatina, Alegre, Vila Velha, Vitória and Serra. The study included 378 dogs, 226 females and 152 males, of various breeds and ages (mean 4.1 years) with ectoparasites (ticks) or parasitism recent history (30 days). Of the 378 dogs examined, 157 (41.53%) had ticks at the time of consultation, which were identified as R. sanguineus s.l. in 154 animals (98.1%), Amblyomma ovale in one animal (0.63%) and Amblyomma sculptum in 2 animals (1.27%). All animals were examined and whole blood were collected and processed by molecular tests: Real Time PCR for Ehrlichia canis, and conventional PCR protocols for Babesia spp., Hepatozoon spp., and for Anaplasmataceae. In the Real Time PCR specific for E. canis, 28 animals (7.4%) were positive. For Anaplasmataceae, 34 animals (9%) were positive, of which 10 generate DNA sequences with 100% similarity with Ehrlichia canis and the other 24 samples generated fragments 100% identical to Anaplasma platys. In the PCR for Babesia spp., 5 animals (1.3%) were positive, producing DNA sequences 100% identical to Babesia vogeli. Finally, 39 animals (10.31%) were positive by PCR for Hepatozoon spp., producing sequences 100% identical to Hepatozoon canis. It was observed coinfection with 2 or 3 agents in 22 animals (5.8%). Of the 378 dogs in the study, 312 had serum samples collected for serological tests: indirect immunofluorescence assay (IFA) for E. canis, with positive reaction in 71 animals (22,75%) and five species of Rickettsia (R. rickettsii, R. parkeri, R. amblyommatis, R. rhipicephali and R. bellii). Among the sera analyzed by IFA, 16 animals (5.13%) had titers ≥ 64 for at least one species of Rickettsia of the five tested, and 5 (1.6%) of those had titers ≥ 64 for R. rickettsii. These results indicate that some of the studied municipalities have agents transmitted by ticks circulating in the canine population, such as E. canis, A. platys, B. vogeli, H. canis, and possibly spotted fever group rickettsiae.