Biotecnologia

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http://repositorio.ufes.br/handle/10/17565
Programa de Pós-Graduação em Biotecnologia

Centro: CCS
Telefone: (27) 3335 7447
URL do programa: http://www.biotecnologia.ufes.br/pt-br/pos-graduacao/PPGBIOTEC

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Navegando Biotecnologia por Autor "Abreu, Gláucia Rodrigues de"

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    Efeitos do tratamento com L-arginina e benazepril isolados e em associação sobre músculo cardíaco de ratos com hipertensão renovascular
    (Universidade Federal do Espírito Santo, 2022-08-26) Silva, Otávio Lélio de Jesus da; Gouvea, Sonia Alves; https://orcid.org/000000015180471X; http://lattes.cnpq.br/7268228122543743; https://orcid.org/0000-0003-1779-4145; http://lattes.cnpq.br/1504092955740146; Abreu, Gláucia Rodrigues de; https://orcid.org/0009-0008-8772-8470; http://lattes.cnpq.br/0229590907405570; Endlich, Patrick Wander; https://orcid.org/0000-0002-7597-3603; http://lattes.cnpq.br/9669721267985878
    Hypertension is the result of blood pressure disorders and can be caused by several factors such as sedentary lifestyle, poor diet and even insufficient hours of sleep. It is also related to kidney disease influenced by physiological factors, which are related to the renin-angiotensin-aldosterone process responsible for regulating blood water volume through sodium levels and also disorder in the sympathetic nervous system. The two-kidney, one-clip experimental model (2K1C) promotes renovascular hypertension, in addition to left ventricular hypertrophy, endothelial dysfunction, and increased levels of reactive oxygen species in the body (ROS), and ischemic heart disease. Treatments with angiotensin-converting enzyme (ACE) inhibitors and supplementation with precursors that potentiate NO synthesis may be one of the alternatives in the control of renovascular hypertension. Renovascular hypertension was induced in Wistar rats with a silver clip implanted in the left renal artery and the animals were divided into the following experimental groups: SHAM, 2-kidneys, 1-clip (2K1C), 2K1C treated with benazepril (BEN), 2K1C treated with L-arginine (L-ARG) and 2K1C treated with an association of Benazepril+L-arginine (Association), treatments started 7 days after clipping and lasted for 3 consecutive weeks. Blood pressure was measured at the beginning, seven days after clipping and at the end of the treatment (28º). After 21 days of treatment, only the BEN+L-ARG group was effective in normalizing systolic blood pressure when compared to the 2K1C and SHAM groups. Our findings also show that the treatments were effective in improving blood pressure in rats with renovascular hypertension. The antioxidant effect of benazepril and its ability to reduce oxidative stress and the supply of NO by Larginine may be an important mechanism for the improvement in vascular remodeling found in our studies. Therefore, our work demonstrates that treatment with BEN associated with L-arginine was effective in reducing BP and preventing hypertrophy, in addition, we point out that the mechanisms involved in these effects seem to be related to a reduction in oxidative stress in the increase of NO bioavailability.
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    Novas abordagens no diagnóstico não invasivo de câncer bucal utilizando espectroscopia ftir em amostras de sangue e saliva
    (Universidade Federal do Espírito Santo, 2021-09-29) Thebit, Marcela Marçal; Gouvea, Sonia Alves; https://orcid.org/000000015180471X; http://lattes.cnpq.br/7268228122543743; https://orcid.org/0000-0002-7305-5753; http://lattes.cnpq.br/7117284804597883; Abreu, Gláucia Rodrigues de; https://orcid.org/0009-0008-8772-8470; http://lattes.cnpq.br/0229590907405570; Endlich, Patrick Wander; https://orcid.org/0000-0002-7597-3603; http://lattes.cnpq.br/9669721267985878; Carvalho, Luis Felipe das Chagas e Silva de; https://orcid.org/0000-0003-1063-4624; http://lattes.cnpq.br/5669726117269568; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108
    Oral cancer is usually diagnosed through invasive methods, for although systemic changes can be easily detected, the identification of an effective systemic biomarker using a simple and affordable approach has yet to take place. Attenuated total reflection Fourier transform infrared (ATR-FTIR) appears as an alternative for the chemical and structural characterization of organic and inorganic materials, including liquid samples as serum, plasma and saliva and a possible bridge between molecular analysis and clinical practice. Objective: The aim of this study was to evaluate if ATR-FTIR spectroscopy can be applied as a tool in oral cancer screening through the analysis of plasma and salivary samples. Methodology: Plasma and saliva samples from oral cancer patients and controls were analyzed by ATR-FTIR spectroscopy. Experimental data were fed into the MATLAB 2018b software and them analyzed through two different routines in order to identify points of differentiations between the groups. Results: Biochemical analysis of the plasma samples through ATR-FTIR yielded 92% sensitivity and 100% specificity in differentiating between groups. The biggest difference was found between peaks that might be related to nucleic acids and nucleic acid phosphate. Pilot study with salivary samples reached similar accuracy Conclusion: We conclude that ATR-FTIR successfully discriminated between oral cancer patients and healthy subjects using plasma samples, with nucleic acids being found as major biomarkers.