Mestrado em Biologia Vegetal

URI Permanente para esta coleção

http://repositorio.ufes.br/handle/10/17506

Nível: Mestrado Acadêmico
Ano de início: 2002
Conceito atual na CAPES: 4
Ato normativo: Homologado pelo CNE (Portaria MEC Nº 609, de 14/03/2019. Publicação no DOU 18 de março de 2019, seç. 1, p.52 - Parecer CNE/CES no 487/2018, Processo no 23001.000335/2018-51).
Periodicidade de seleção: Anual
Área(s) de concentração: Filosofia
Url do curso: https://biologiavegetal.ufes.br/pt-br/pos-graduacao/PPGBV/detalhes-do-curso?id=28

Navegar

Navegando Mestrado em Biologia Vegetal por Autor "Andrade, Marcieni Ataíde de"

Agora exibindo 1 - 4 de 4
  • Nenhuma Miniatura disponível
    Item
    Avaliação dos efeitos alelopáticos e mutagênicos de formas extrativas de Passiflora edulis Sims por meio do bioensaio Allium cepa
    (Universidade Federal do Espírito Santo, 2009-12-15) Costalonga, Schirley Aparecida; Batitucci, Maria do Carmo Pimentel; Valéria de Oliveira Fernandes; Andrade, Marcieni Ataíde de
    The human being use plants for different things, like powerful medicine instrument and biological control. The Allium cepa system is a efficient tool for identify dangerous substances for animals and vegetables. Passiflora edulis is a native plant used abundantly as phitoteraphic in Brazil; besides it produce many substances with allelophatic potencial, but without intense studies. Thus, the aim was evaluated the allelophatic and mutagenic effects of four leave extracts and two infusions of P. edulis over A. cepa seeds treaty with continuous and discontinuous acute and chronics treatments in middle with water, three concentration of extracts (1, 2 e 5 mg/mL) or three concentrations of infusions (10, 50 e 100%). Happened a accentuated allelophatic potential for the ethanolic extract, which reduced the germination and speed’s germination index; for the hexane and acetate extracts, same results were obtained for 2 e 5 mg/mL concentration and for dichlorometane extract, only in the major concentration. Didn’t have changes on this parameters when mesocárpico and leave infusions were used. For the roots developments, none change was reported. The mitotic index was affected in all three extracts’ concentrations, in 100% of continuous treatments, all concentration of acute discontinuous and 10 e 100% of chronic treatment with mesocarpic infusion. Aneugenic effects were related only in 1 mg/mL of dichlorometane extract and 100% acute discontinuous treatment of leave infusion. Clastogenic effects happened in 2 mg/mL of acetate extract in acute discontinuous treatment and 2 mg/mL of ethanolic extract in continuous treatment. The seeds treated with 1 e 2 mg/mL of acetate extract for 72h presented abnormal roots growth and didn’t response for coloration. Happened elevated number of dead cells and in all chronic discontinuous treatment. The results above denote the deep relation between the allelophatic and mutagenic effects and reinforce the importance of study the native species. For more security concerning the use of P. edulis for human beings, advice the establish tests in animals
  • Nenhuma Miniatura disponível
    Item
    Avaliação morfofisiológica, fitoquímica e mutagênica de Passiflora edulis Sims f. flavicarpa Deg exposta a diferentes concentações de alumínio
    (Universidade Federal do Espírito Santo, 2012-02-27) Perdigão, Tatiane Lemos; Jamal, Cláudia Masrouah; Batitucci, Maria do Carmo Pimentel; Andrade, Marcieni Ataíde de; Falqueto, Antelmo Ralph
    The influence of aluminum (Al) in two cultivars of Passiflora edulis is presented in two chapters: first a discussion on the experiment with the cultivar (cv.) FB 100 and second with the cultivar (cv.) FB 200. The study with cv. FB100 in greenhouse aimed to verify morphophysiological changes after 15 days of treatment with Al. For cv. FB 200 plants was evaluated the relationship between the mutagenic and the concentration flavonoids produced by them, when grown in soils with different concentrations aluminum. Analyses of fresh and dry weight organs, stem length, leaf area, nutritional analysis and verification of Al in leaves and roots were studied on cv.FB100. For the leaves of cv. FB100 and cv. FB200 phytochemical screening of extracts were used staining and precipitation reactions for identification of the main chemical classes present. The concentration of the flavonoids was determined by spectrometric measurement. To mutagenicity test was found the frequency of micronucleus in 2000 polychromatic erythrocytes using the cv. FB200 leaves extract. All cv. FB100 morphological parameters analyzed did not differ from controls. However a higher Al concentration was presented in the treatment of 5.32 mM roots. All treatments showed the presence of alkaloids, flavonoids and steroids in leaves. The extract hexane/dichloromethane at a higher flavonoid concentration, has been observed in treating 0.33 mM Al. The cv. FB200 results showed higher foliar Al concentration in the field with 0.33 mM Al (T2), and saponins, steroids and flavonoids were the major groups found. The T2 treatment had the highest flavonoids concentration and higher mutagenic potential. These results indicate that Al did not promote significant changes in cv. FB 100 morphophysiological parameters. In P.edulis cv. FB 200 saponins, flavonoids and steroids occur regardless of the Al available and possibly the largest concentration of flavonoid mutagenicity can be induced in cells of mouse bone marrow
  • Nenhuma Miniatura disponível
    Item
    Estudos fitoquímico, alelopático, tóxico e mutagênico de Erythrina mulungu MART. ex BENTH. utilizando bioensaios
    (Universidade Federal do Espírito Santo, 2009-02-27) De Bona, Ana Paula; Andrade, Marcieni Ataíde de; Batitucci, Maria do Carmo Pimentel; Fernandes, Valéria de Oliveira; Cunha, Marcia Regina Holanda da
    The plants produce a range of secondary compounds, which can act as active principle in drugs or can produce toxic effect on animals and other plants. Thus, as the Erythrina mulungu (Mart. ex Benth.) a kind of pharmacological and ecological importance, this study aims to evaluate the structure phytochemistry and allelopathic and mutagenic effects potential of the crude extract of the inflorescences and leaves of this plant through of different bioassays. The phytochemical survey was conducted through chemical reactions that resulted in the development of color and/or precipitate characteristic. The allelopathic tests in Allium cepa were conducted and consisted of 8 treatments (distilled water and 0025; 0.05, 0.1, 0.2, 0.3, 0.4 and 0.6 mg/mL of extracts) and 5 repetitions with 20 seeds each. Evaluated the germination speed, the first count, the germination percentage and the media root. For analysis cytotoxic and mutagenic in plants, seeds of Allium cepa were subjected to continuous and discontinuous treatment (20 and 72 hours) and assessed the mitotic index, aneugenic effect index and clastogenic effect index. The acute toxicity in animals was achieved through the establishment of the median lethal dose (LD50) and cytotoxic and mutagenic analysis made by the micronucleus test, using the method of smear, after exposure of animals to 5 days of treatment. The results show the presence of reducing sugars, phenols and tannins, proteins and amino acids, alkaloids, flavonoids, depsides and depsidones, derived from coumarin in both organs; saponins, steroids and triterpenes in the leaves and cardiac and anthraquinones glycosides in inflorescences. The bodies have allelopathic activity on the root development, potential cytotoxic and no aneugenic effect on the onion. The inflorescences have potential clastogenic. To the LD50, the leave showed non toxic and inflorescence, moderately toxic. Both showed lack of cytotoxic and genotoxic potential in cells of rodents. The use ofE. mulungu, both for the management of degraded or agricultural areas, as for the manufacture of medicines, must be done so cautiously
  • Nenhuma Miniatura disponível
    Item
    Prospecção fitoquímica e avaliação dos efeitos biológicos do extrato etanólico das partes aéreas de Pilea microphylla (L.) Liebm. (Urticaceae): aspectos tóxico, mutagênico, antimutagênico e antioxidante
    (Universidade Federal do Espírito Santo, 2009-02-26) Gomes, Tarsila Daysy Ursula Hermogenes; Andrade, Marcieni Ataíde de; Batitucci, Maria do Carmo Pimentel; Silva, Diolina Moura; Antunes, Lusânia Maria Greggi
    Pilea microphylla (L.) Liebm. (Urticaceae), popularly known as brilhantina in Brazil, is used as antipyretic, purifying the bladder, anti-diarrhea, anti-asthma and used for abdominal pain. Despite this medical use, there is no information about its biology and therapeutic efficacy. The objective of this work is to characterize the phytochemical, quantifying the total phenolic composition and total antioxidant capacity, and assess possible effects of the acute toxicity by LD50 (Median Lethal Dose), mutagenic and antimutagenic by micronucleus test in bone marrow and peripheral blood of mice. The dried aerial parts were subjected to maceration in absolute alcohol, resulting the crude ethanolic extract (CEE). The qualitative phytochemical prospection indicated the presence of reducing sugars, phenols and tannins, depsides and depsidons, coumarins, steroids and triterpenes. The concentration of phenolic compounds was 9.75 tannic acid equivalents and gallic acid equivalents 17.5 (reading in spectrophotometer), lower than the result found by other authors with other plant species. The antioxidant activity showed values similar or even higher than those found for other plant species. The test indicated no acute toxicity LD50, as obtained by another study. Nevertheless, the test for mutagenicity of CEE in doses 250 mg/Kg and 500 mg/Kg in bone marrow and peripheral blood of mice showed no significant increase in the frequency of micronuclei in polychromatic erythrocytes (MNPCEs) and normochromatic (MNNCEs) in marrow and peripheral blood, respectively, independent of concentration. The antimutagenic potential was evaluated in dose 250 mg/Kg in pre-treatment, post-treatment and simultaneous treatment. Only the post-treatment showed a decrease not significant in the frequency of MNPCEs on the positive control. In peripheral blood, it was found that post-treatment had lower values of MNNCEs. The results suggest that the indiscriminate and prolonged use (subchronic/chronic) preparations of Pilea microphylla may be harmful to health, due to the formation of micronuclei, but provide information for other studies to confirm the antimutagenic effect, to elucidate the mechanisms of mutagenicity and phytochemical composition, as important sources of bioactive compounds for the pharmaceutical industry for use in an isolated manner or in association with conventional treatments in various diseases such as cancer.