Biologia Vegetal
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Programa de Pós-Graduação em Biologia Vegetal
Centro: CCHN
Telefone: (27) 4009 2588
URL do programa: http://www.biologiavegetal.ufes.br/
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- ItemDesinfestação, estabelecimento e calogênese in vitro de Paubrasilia echinata Lam E. Gagnon, H. C. Lima & G. P. Lewis(Universidade Federal do Espírito Santo, 2024-08-09) Mariano, Gustavo Fernandes; Co-orientador1; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador2; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador3; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador4; ID do co-orientador4; Lattes do co-orientador4; Orientador1; https://orcid.org/; http://lattes.cnpq.br/; Orientador2; https://orcid.org/; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; 1º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 2º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 3º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 4º membro da banca; http://lattes.cnpq.br/; 5º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 6º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 7º membro da banca; https://orcid.org/; http://lattes.cnpq.br/The species Paubrasilia echinata, known worldwide as pau-brasil and considered the national tree of Brazil, is a tree species belonging to the Fabaceae family and native to the Atlantic Forest. P. echinata is endangered due to intense exploitation, driven by the high demand for its wood during the colonial period in Brazil, making it necessary to develop strategies for its conservation, propagation, and reforestation, especially considering its representative role in the Atlantic Forest biome. In this context, in vitro propagation or micropropagation emerges as an excellent alternative for the species' preservation. However, native species face challenges in establishing in vitro due to the difficulty of disinfecting biological material (explants) to eliminate microorganisms originating from the field. These microorganisms, usually bacterial or fungal in nature, can proliferate excessively in the in vitro environment and lead to the death of the explant. Tests with different explants, different exposure times, and different concentrations of conventional disinfecting agents (alcohol and sodium hypochlorite), as well as new methodologies such as the use of silver nanoparticles (AgNPs), have been developed to improve the disinfection technique of explants and favor the in vitro establishment of tree species like pau-brasil. Therefore, the present study aimed to test the disinfecting agents sodium hypochlorite (NaClO) from the Qboa® brand, silver nanoparticles (AgNPs), and their combination, at different exposure times (5- 30 min) and concentrations (60 and 100%) using fruits, seeds, and axillary buds as explants for the disinfection and callogenesis induction stages with P. echinata from leaflets of in vitro plants and zygotic embryos with different growth regulators (2,4-D, Dicamba, and Picloram). The results showed that the disinfection of P. echinata fruits treated in a solution containing only NaClO and Manzate® fungicide had contamination rates below 25%, with the lowest percentage of contamination. For in vitro germination percentage, the values reached 75% compared to the other explants and treatments containing NaClO and AgNPs, offering more effective disinfection and a more suitable in vitro establishment of the species, providing in vitro biological material for various uses and purposes in in vitro propagation. For callogenesis induction, the formation of non-embryogenic calli was observed in all treatments
- ItemPotencial bioestimulante do extrato de microalgas na germinação e crescimento de culturas agrícolas(Universidade Federal do Espírito Santo, 2022-10-05) Santos, Nair Hildelgard Soares dos; Machado, Levi Pompermayer; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador2; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador3; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador4; ID do co-orientador4; Lattes do co-orientador4; Fernandes, Valéria de Oliveira ; https://orcid.org/; http://lattes.cnpq.br/; Orientador2; https://orcid.org/; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; Corte, Viviana Borges ; https://orcid.org/; http://lattes.cnpq.br/; Almeida, Stéfano Zorzal de ; https://orcid.org/; http://lattes.cnpq.br/; Alves, Fernanda Brêda ; https://orcid.org/; http://lattes.cnpq.br/; Dutra, Jean Carlos Vencioneck ; http://lattes.cnpq.br/; 5º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 6º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 7º membro da banca; https://orcid.org/; http://lattes.cnpq.br/Currently the global demographic pressure on agricultural production requires new and sustainable approaches to meet the growing demand for plant biomass for human feed, animal feed and energy production. Therefore, there has been growing interest in natural biostimulant substances in agriculture due to the challenge of balancing technological development with environmental conservation. Microalgae have several biotechnological applications, including biostimulants. In modern agriculture, microalgae are an ecologically correct option to replace chemical fertilizers, as they can be used as biostimulants, soil modifiers and food additives. The objective of this work was to evaluate the effects of the application of microalgae extracts on the germination and growth of bean (Phaseolus vulgaris L.), corn (Zea mays L.) and papaya (Carica papaya L.) crops. It was possible to evidence that aqueous extracts obtained from microalgae presented biostimulating growth potential, considering that all seeds used in this study showed positive germ responses. It is noteworthy that most seeds obtained higher germination values, mean time, good speed indices, and better initial performance when submitted to extracts of species of the genus Chlorella, mainly at concentrations 1.0 g/L and 1.5 g/L. For the growth experiment, Scenedesmus acuminatus extract showed efficacy in stimulating the growth of bean plants, considering that plants submitted to this extract obtained higher grain yield per plant and higher number of pods. Thus, it can be affirmed that the biostimulant, when applied by foliar, is capable of promoting the transport of photoassimilates for the seeds, which would result in greater accumulation of mass in the seeds. It is also possible to attribute the positive effect of S. acuminatus extract on the yield and productivity of the bean crop to the availability of micronutrients present in this microalgae. It is necessary to highlight that to evaluate the action of extracts produced from algae, one should consider the agricultural crop to be tested, and concentrations. Because the seeds may present a different physiology and this influences the mechanism of action of the extracts, consequently presenting different responses from those obtained in this study. From the results it is possible to attribute the importance of microalgae in the agricultural sector, for its potential for the development of new biostimulating products of plant growth