Biologia Vegetal
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Programa de Pós-Graduação em Biologia Vegetal
Centro: CCHN
Telefone: (27) 4009 2588
URL do programa: http://www.biologiavegetal.ufes.br/
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- ItemDesinfestação, estabelecimento e calogênese in vitro de Paubrasilia echinata Lam E. Gagnon, H. C. Lima & G. P. Lewis(Universidade Federal do Espírito Santo, 2024-08-09) Mariano, Gustavo Fernandes; Co-orientador1; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador2; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador3; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador4; ID do co-orientador4; Lattes do co-orientador4; Orientador1; https://orcid.org/; http://lattes.cnpq.br/; Orientador2; https://orcid.org/; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; 1º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 2º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 3º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 4º membro da banca; http://lattes.cnpq.br/; 5º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 6º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 7º membro da banca; https://orcid.org/; http://lattes.cnpq.br/The species Paubrasilia echinata, known worldwide as pau-brasil and considered the national tree of Brazil, is a tree species belonging to the Fabaceae family and native to the Atlantic Forest. P. echinata is endangered due to intense exploitation, driven by the high demand for its wood during the colonial period in Brazil, making it necessary to develop strategies for its conservation, propagation, and reforestation, especially considering its representative role in the Atlantic Forest biome. In this context, in vitro propagation or micropropagation emerges as an excellent alternative for the species' preservation. However, native species face challenges in establishing in vitro due to the difficulty of disinfecting biological material (explants) to eliminate microorganisms originating from the field. These microorganisms, usually bacterial or fungal in nature, can proliferate excessively in the in vitro environment and lead to the death of the explant. Tests with different explants, different exposure times, and different concentrations of conventional disinfecting agents (alcohol and sodium hypochlorite), as well as new methodologies such as the use of silver nanoparticles (AgNPs), have been developed to improve the disinfection technique of explants and favor the in vitro establishment of tree species like pau-brasil. Therefore, the present study aimed to test the disinfecting agents sodium hypochlorite (NaClO) from the Qboa® brand, silver nanoparticles (AgNPs), and their combination, at different exposure times (5- 30 min) and concentrations (60 and 100%) using fruits, seeds, and axillary buds as explants for the disinfection and callogenesis induction stages with P. echinata from leaflets of in vitro plants and zygotic embryos with different growth regulators (2,4-D, Dicamba, and Picloram). The results showed that the disinfection of P. echinata fruits treated in a solution containing only NaClO and Manzate® fungicide had contamination rates below 25%, with the lowest percentage of contamination. For in vitro germination percentage, the values reached 75% compared to the other explants and treatments containing NaClO and AgNPs, offering more effective disinfection and a more suitable in vitro establishment of the species, providing in vitro biological material for various uses and purposes in in vitro propagation. For callogenesis induction, the formation of non-embryogenic calli was observed in all treatments
- ItemEcofisiologia, fenologia e adaptação de espécies florestais arbóreas nas condições edafoclimáticas de Viana-ES(Universidade Federal do Espírito Santo, 2014-07-11) Barbosa, Jesus Fernando Miranda; Silva, Diolina Moura; Ventura, José Aires; 1º membro da bancaThe intense human activity devastates large native forests areas, either for agriculture expansion or to supply the growing wood market; anyway, the small forest fragments remaining suffer constant anthropic pressure. However, the use of adapted tree species may contribute to the natural resources protection. This present study aimed the evaluation of phenological patterns from 109 forest tree species, belonging to 37 families and 82 genders, between native and exotic, with 9 years old, to differentiate the most adapted and with potential to be used in reforesting programs. The search was conducted at Incaper’s Experimental Farm, in Jucuruaba, Viana-ES city, (UTM E-345524, N-7741039). It was performed soil chemical analysis of the planted area and the weather data was obtained in Viana’s meteorological station. The study was based on the observation of the number of surviving plants from each specie and its growth evaluation. Observations of the budding phenophases, leaves senescence, flowering and fruiting were performed. Phenological evaluations were developed monthly, from November 2012 to October 2013. It were collected data from the measurement of tree height, diameter at breast height (DBH), index of foliage, survival rate and calculating the CGI (Combined Growth Index), as well as the determination of the most appropriate species. From the 109 species studied, 64.22% showed functional and structural adaptation to soil and climate conditions of the experimental region; 42.22% flowered and fruited; and 90% showed senescence and budding following seasonality. Twenty-nine species showed a GIC higher than the average. Most species stood out as an alternative to local vegetation recovery, especially Inga uruguensis and Schizolobium amazonicum. The chlorophyll fluorescence analysis revealed that the photosynthetic apparatus of Schizolobium amazonicum was able to protect it from photoinhibition and promote good conversion of light energy into photochemical.
- ItemIdentificação de mecanismos relacionados à resistência à fusariose do abacaxizeiro(Universidade Federal do Espírito Santo, 2009-02-12) Zorzal, Poliana Belisário; Fernandes, Patricia Machado Bueno; 1º membro da banca