Mestrado em Genética e Melhoramento
URI Permanente para esta coleção
Nível: Mestrado Acadêmico
Ano de início: 2013
Conceito atual na CAPES: 5
Ato normativo: Homologado pelo CNE/CES Parecer nº 250/2014, portaria nº 187 de 06/03/2015, publicado no DOU de 09/03/2015 seção 1, página 11
Periodicidade de seleção: Semestral
Área(s) de concentração: Genética e Melhoramento
Url do curso: https://geneticaemelhoramento.ufes.br/pt-br/pos-graduacao/PPGGM/detalhes-do-curso?id=1415
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Navegando Mestrado em Genética e Melhoramento por Autor "Bento, Cintia dos Santos"
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- ItemAcessos de Capsicum spp. com potencial ornamental e resistência a múltiplas doenças(Universidade Federal do Espírito Santo, 2021-10-15) Alves, Thayllon de Assis; Bento, Cintia dos Santos; https://orcid.org/0000000316394166; http://lattes.cnpq.br/4236145191829946; https://orcid.org/000000032478851X; http://lattes.cnpq.br/0420240855848079; Sudré, Cláudia Pombo; https://orcid.org/0000-0001-6743-7205; http://lattes.cnpq.br/4185732042391423; Xavier, André da Silva; https://orcid.org/0000000292510301; http://lattes.cnpq.br/5661020509713522Peppers, belonging to the genus Capsicum L., have been cultivated for a long time in family farming, as they have a good economic return, due to their diversification of use. In recent times, peppers are no longer only cultivated for the culinary, pharmaceutical and cosmetics market and have also started to stand out in the ornamental plant market, mainly due to their variability in color and shape of fruits and leaves. However, the emergence of diseases, caused by different phytopathogens, represents one of the limiting factors for the production of this crop. Therefore, the objective of this work is to identify accessions of Capsicum spp. belonging to the BAG - active germplasm bank of the Federal University of Espírito Santo -Brazil, Campus de Alegre, with ornamental potential and resistant to multiple diseases. To assess the ornamental potential,were used, 42 accessions of Capsicum spp. and the hot pepper cultivar for pot, as a control, totaling 43 genotypes. The morphoagronomic evaluations were performed using the descriptor for Capsicum from Bioversity International (IPGRI). For the selection of genotypes, the analysis of variance and the Scott-Knott mean comparison test were performed. To evaluate disease resistance, were used, 41 accessions and two controls were evaluated, cultivar Ikeda (negative control) and UENF 1381 (positive control), totaling 43 genotypes. For the resistance evaluation, a completely randomized experimental design was used. For the inoculation of Xanthomonas, the Isolate ENA 4135 was used, at concentrations of 108 CFU/mL, for the hypersensitivity reaction, and 105 CFU/mL for the quantitative evaluation. The assessment was performed using a grading scale, suggested by Riva-Souza (2009). For resistance to anthracnose, five fruits were inoculated, in the both immature and mature stages, of each plant, using a suspension of 106 conidia/mL. The assessment was carried out using the grading scale suggested by Montri et al. (2009). To identify resistance to PepYMV, accessions were kept under protected conditions, in cages lined with an anti-aphid screen, to prevent transmission of the virus to other plants in the area. The plants were visually evaluated, using the grading system from 1 to 5. For data analysis, the Area Below the Disease Progress Curve (AUDPC) and the incubation period were used. Genotypes with ornamental potential were found, both for potted cultivation and for outdoor garden ornamentation. Genotype 23 had the best characteristic for potted trade, and genotype 52 for garden ornamentation. Genotypes with resistance to the three diseases were also observed, these were 56 and 82 for all variables and 25 showed resistance for all variables except for anthracnose in unripe fruit. Other genotypes were characterized for simple resistance to one disease, and in some genotypes, resistance to two of the studied diseases was observed.
- ItemMoringa oleifera Lam: caracterização molecular(Universidade Federal do Espírito Santo, 2023-12-15) Silva, Ronei Costa; Bento, Cintia dos Santos; https://orcid.org/0000000316394166; http://lattes.cnpq.br/4236145191829946; http://lattes.cnpq.br/7091004633430310; Sudre, Claudia Pombo; Moulin, Monique Moreira; http://lattes.cnpq.br/8654999153826715Moringa oleifera Lam is a medium-sized tree belonging to the Moringaceae family, native to South Asia. It is popular in different parts of the world for its diverse nutritional and medicinal uses. Moringa is cultivated in various parts of the world and is well adapted in many regions, which provides greater variability of the species. Therefore, this study aimed to evaluate the genetic diversity among 10 genotypes of M. oleifera using 15 ISSR primers and 20 SSR primers. The experiment was conducted in a greenhouse at the Federal University of Espírito Santo (UFES) Alegre campus and at the Federal Institute of Espírito Santo, Alegre campus. The material was collected and donated. The seeds were sown in 290 cm³ tubes containing commercial substrate in order to generate seedlings to obtain plant material for molecular characterization. For the evaluation of genetic diversity, 10 genotypes of M. oleifera were analyzed. When the seedlings presented two to four pairs of definitive leaves, they were collected for DNA extraction using the Doyle and Doyle protocol adapted by the IAC. The genetic distance matrix was estimated through quantitative variables based on the Standardized Average Euclidean distance, and the genotypes were grouped using the Unweighted Paired Group Method using Arithmetic Averages (UPGMA) and validated using the cophenetic correlation coefficient. These procedures were performed for both SSR and ISSR primers. With the results obtained, it was possible to identify the formation of three distinct groups using the ISSR marker and the formation of four distinct groups using the SSR primers. In this work, it was possible to estimate the genetic variability among the studied genotypes, making it possible to make genotypes available for future M. oleifera genetic improvement programs. The ISSR primers were more efficient in discriminating the collected genotypes.
- ItemÓleo essencial de Lavandula hybrida Reverchon no controle da antracnose em frutos do gênero Capsicum(Universidade Federal do Espírito Santo, 2023-05-15) Silva, Mateus Mendes da; Bento, Cintia dos Santos; https://orcid.org/0000000316394166; http://lattes.cnpq.br/4236145191829946; https://orcid.org/0000000335610397; http://lattes.cnpq.br/1774047030676800; Moraes, Willian Bucker; https://orcid.org/0000000174787772; http://lattes.cnpq.br/6727861982577995; Moulin, Monique Moreira; https://orcid.org/0000-0001-8993-4835; http://lattes.cnpq.br/8654999153826715The misuse of synthetic products in agriculture causes damage to the environment and human health, which leads to the search for less harmful compounds to the environment and to man. Studies with natural products prove the efficiency of essential oils in controlling diseases in agricultural crops, it is possible to reduce production costs, preserve the environment and reduce chemical contamination in food. The objective of this work was to characterize, chemically the essential oil of lavandin and evaluate the efficiency of the oil, as well as its major compounds, in the control of Colletotrichum scovillei in vitro and in vivo, in fruits of peppers of the genus Capsicum. The properties and major substance(s) of lavandin essential oil were identified through chemical analysis. The efficiency of the essential oil in the control of C. scovillei, was evaluated from preliminary tests in vitro, using the concentrations of 1 µl/mL, 4 µl/mL, 8 µl/mL, 12 µl/mL, 16 µl/mL, 20 µl/mL and 24 µl/mL in order to determine the inhibitory concentrations to the fungus. The essential oil was used in the form of an emulsion. The best results were used for the in vivo assay. As a positive control for the development of anthracnose, a commercial fungicide was used, recommended for culture, Azoxystrobin (200 g/L) + Difenoconazole (125 g/L), in the Suspension Concentrate (SC) formulation, as a negative control, the emulsifier Tween 80 was used. In this step, five fruits in the immature stage of Capsicum spp. of the Ikeda cultivar, susceptible to anthracnose. After applying the emulsions, the fruits were pierced with the aid of a needle, to facilitate penetration of the fungus into the fruits and inoculated with a suspension of 1.0 x 106 conidia/mL. After inoculation, the fruits were placed in a humid chamber and kept at room temperature. After obtaining data on the inhibition of mycelial growth of C. scovillei in the in vitro test and the severity of the disease in the in vivo test, statistical analyzes that best represent the data were performed. Chemical analysis of lavandin essential oil revealed the predominance of chemical compounds linalool and linalool acetate. Lavandin essential oil and the major compounds linalool and linalool acetate were effective in controlling C. scovillei, under in vitro conditions, at concentrations 4.00 µL/mL, 1.45 µL/mL and 24 µL/mL, respectively. Linalool was more effective in inhibiting the mycelial growth of C. 10 scovillei, presenting the lowest CIs (CI 50 and CI 90). With the results obtained, it was possible to detect the inefficiency of lavandin essential oil in controlling the growth of C. scovillei in Capsicum fruits, of the Ikeda cultivar. Therefore further studies are needed: suitable protocols for emulsion storage, its mechanisms of action to ensure effectiveness of this ecologically correct product in antifungal control and use of higher concentrations.