Doutorado em Ciências Florestais

URI Permanente para esta coleção

http://repositorio.ufes.br/handle/10/17466

Nível: Doutorado
Ano de início: 2013
Conceito atual na CAPES: 5
Ato normativo: Portaria nº 398 de 29 de maio de 2025, publicado no DOU de 02/06/2025. Homologação do Parecer CNE/CES nº 176/2025
Periodicidade de seleção: Semestral
Área(s) de concentração:Ciências Florestais
Url do curso: https://cienciasflorestais.ufes.br/pt-br/pos-graduacao/PPGCFL/detalhes-do-curso?id=1425

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Navegando Doutorado em Ciências Florestais por Autor "Alexandre, Rodrigo Sobreira"

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    Conservação Ex Situ E Tolerância À Dessecação Em Sementes De Lecythis Pisonis Cambess
    (Universidade Federal do Espírito Santo, 2024-02-23) Araujo, Caroline Palacio de; Alexandre, Rodrigo Sobreira; https://orcid.org/0000-0002-5248-6773; http://lattes.cnpq.br/5340049196888351; https://orcid.org/0000-0002-0259-2543; http://lattes.cnpq.br/1424851890250661; Santos, Heloisa Oliveira dos; https://orcid.org/0000-0003-1384-4969; http://lattes.cnpq.br/7905575260641836; Lopes, José Carlos; https://orcid.org/0000-0002-4880-0547; http://lattes.cnpq.br/1183524198654764; Pereira, Wilson Vicente Souza ; https://orcid.org/0000-0002-7931-8382; http://lattes.cnpq.br/2641981653845321; Maciel, Khétrin Silva ; https://orcid.org/0000-0002-8590-7917; http://lattes.cnpq.br/6753673403909545
    The species Lecythis pisonis Cambess. is a chestnut tree belonging to the Lecythidaceae family, commonly found in the Atlantic Forest and Amazon biomes. Studies related to its propagation and ex situ conservation are important, contributing to the protection of its genetic resources over time. This research answers important questions related to the propagation of the seminiferous species L. pisonis, whose objectives were a) Chapter I – to study fixed temperatures and thermal shocks at different exposure times on the physiology of seeds and initial growth of L. pisonis seedlings; b) chapter II – analyze the influence of seed size and shading on seedling emergence and the phenotypic and photosynthetic plasticity of L. pisonis seedlings; c) chapter III – analyze tolerance to desiccation and maintenance of seed viability of the chestnut tree L. pisonis; and d) chapter IV – analyze the physiological quality of L. pisonis seeds during ex situ storage under different temperature conditions and time variations. Chapter I was divided into experiment 1 (fixed temperatures 30; 35; 40; 45 and 50 °C and thermal shocks at temperatures 30-35; 30-40; 30-45; 30-50; 35-40; 35-45; 35-50; 40-45; 40-50 and 45-50 °C at exposure times of 20 h/4 h and 21 h/3 h) and experiment 2 (fixed temperatures 30 and 45 °C and thermal shock 30-45 °C at exposure times 23.5 h/0.5 h; 23 h/1 h; 22 h/2 h; 21 h/3 h; 19 h/5 h; 18 h/6 h and 17 h/7 h ). Both were conducted in a completely randomized design, with four replications of 25 seeds each, totaling 100 seeds/treatment. In Chapter II, the treatments were arranged in a randomized block design and split-plot system, analyzing seed mass (small: 4 to 5.9 g; medium: 6 to 7.9 g and large: 8 to 9.9 g) x reduction in light capacity (0; 30; 50; 70 and 80%), using four repetitions of 25 seeds each, totaling 100 seeds/treatment. In Chapter III, the treatments were arranged in a randomized block design, consisting of treatments with different water contents in the seeds (30%, 15%, 10%, and 4%). Then, part of the seeds with a water content of 4% was stored in aluminum containers for three months at freezer (-20) and ultrafreezer (-86 °C) temperatures. Each treatment consisted of 100 seeds, organized in four replications of 25 seeds each. Chapter IV was divided into two storage experiments, with L. pisonis seeds placed in Kraft Nat® paper bags (jute bags) measuring 23.5 x 10 x 31.5 cm and stored in germination chambers of the Biochemical Oxygen Demand (BOD) type, at temperature and relative humidity of 25 °C (RH = 63%); 6 °C (RH = 49%); 2 °C (RH = 31%) and -6 °C (RH = 10%), with the emergence of experiment 1 conducted in BOD and that of experiment 2 in a greenhouse. In Chapter, I, experiment 1 presented efficient results when exposing L. pisonis seeds to thermal shock 30-45 °C (20 h/4 h) and in experiment 2, when subjecting the seeds to thermal shock 30-45 °C (21h/3h). In both cases, there was high seedling emergence, high vigor, and an efficient antioxidant system, with increased activity of the enzymes SOD, CAT, and APX. In Chapter II, it was observed that large seeds showed the highest seedling emergence (83.9%) when exposed to shading of 49.32% (~50%). In Chapter III, seedling emergence decreased depending on the water content in the seeds (30>15>10>4%), varying between 88 and 64% emergence, respectively, which were classified as intermediate seeds. In Chapter IV, experiment 1 obtained greater germination (95%) and seedling emergence (65%) in seeds stored at 2 °C for 11 months. For experiment 2, seeds stored at a temperature of 6 °C achieved seedling emergence rates greater than 70% for most months of storage. It is concluded in Chapter I that the thermal shock of 30-45 °C at exposure times of 20 h/4 h and 21 h/3 h are optimal for the emergence of L. pisonis seedlings. In Chapter II, the use of large seeds (8 to 9.9 g) and 50% shading is recommended for the emergence of L. pisonis seedlings. In Chapter III, it is concluded that L. pisonis seeds have intermediate characteristics. In Chapter IV, it is concluded that it is possible to preserve L. pisonis seeds for one year in storage at a temperature of 6 °C, which are classified as intermediate seeds. Keywords: seeds, germination, oilseed plants, seed storage, seed physiology.
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    Conservação ex situ e tolerância à dessecação em sementes de Lecythis pisonis cambess
    (Universidade Federal do Espírito Santo, 2024-02-23) Araujo, Caroline Palacio de; Alexandre, Rodrigo Sobreira; https://orcid.org/0000-0002-5248-6773; http://lattes.cnpq.br/5340049196888351; https://orcid.org/0000-0002-0259-2543; http://lattes.cnpq.br/1424851890250661; Lopes, José Carlos; https://orcid.org/0000-0002-4880-0547; http://lattes.cnpq.br/1183524198654764; Pereira, Wilson Vicente Souza; https://orcid.org/0000-0002-7931-8382; http://lattes.cnpq.br/2641981653845321; Santos, Heloisa Oliveira dos; https://orcid.org/0000-0003-1384-4969; http://lattes.cnpq.br/7905575260641836; Maciel, Khétrin Silva; https://orcid.org/0000-0002-8590-7917; http://lattes.cnpq.br/6753673403909545
    Plants are sessile life forms, which makes them more susceptible to environmental temperature variations, especially during their reproduction and adaptation. Germination is a sensitive and crucial stage in the life cycle of seed-producing plants and occurs within temperature ranges suitable for the species. The objective of this study was to investigate fixed temperatures and thermal shocks at different exposure times on the physiology of seeds and initial growth of L. pisonis seedlings. Vigor, emergence, seedling growth, chlorophyll fluorescence, chlorophyll a and b index, maximum PS II quantum yield, oxidative stress enzymes, lipid peroxidation, hydrogen peroxide, and DNA methylation in seedlings were evaluated. In experiment 1, L. pisonis seeds exposed to thermal shock 30-45 °C (20 h/4 h) showed high seedling emergence (88%), vigor, and an efficient antioxidant system, with increased activity of SOD, CAT, and APX enzymes. This same treatment showed high concentrations of H2O2, indicating that it may have acted as a signaling molecule favoring seedling emergence. It was also observed in experiment 1 that fixed temperatures of 40, 45, and 50 °C led to loss of seed viability, and that high temperatures for long periods caused decreased vigor, seed deterioration, damage to the photosynthetic apparatus (PS II), and formation of seedlings with absent root systems. In experiment 2, it was observed that the treatment 30-45 °C (21 h/3 h) achieved high percentages of emergence (84%) and vigor in a short period of time (23 days), corroborating with the results of experiment 1. This treatment also provided high activity of SOD, CAT, and APX, evidencing an efficient antioxidant system. In experiment 2, it was observed that the quality of seedlings was affected in the thermal shock 30-45 °C when exposed at a temperature of 45 °C for 5, 6, and 7 hours. It is concluded from this study that L. pisonis seeds do not tolerate long exposure to high temperatures, however, they express greater vigor and seedling emergence when subjected to short periods of time under these conditions. Thermal shock 30-45 °C at exposure times 20 h/4 h and 21 h/3 h are suggested as optimal for the emergence of L. pisonis seedlings.
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    Desenvolvimento, maturação e germinação in vitro de sementes, e embriogênise somática de Euterpe edulis Martius
    (Universidade Federal do Espírito Santo, 2022-02-28) Mello, Tamyris de; Alexandre, Rodrigo Sobreira; https://orcid.org/0000000252486773; http://lattes.cnpq.br/5340049196888351; https://orcid.org/0000000311895404; http://lattes.cnpq.br/9519462640256364; Otoni, Wagner Campos; https://orcid.org/0000-0002-9614-9373; https://orcid.org/http://lattes.cnpq.br/6132560404570245; Santos, Heloisa Oliveira dos; https://orcid.org/0000-0003-1384-4969; http://lattes.cnpq.br/7905575260641836; Carvalho, Virginia Silva; https://orcid.org/0000-0002-8703-286X; http://lattes.cnpq.br/3199872902054955; Schmildt, Edilson Romais; http://lattes.cnpq.br/0648502408490406
    The species Euterpe edulis Martius, is a palm belonging to the Arecaceae family, popularly known as juçara, it is widely distributed in the Atlantic Forest. In recent decades this palm tree has been exploited for its palm heart, and the fact that it grows and propagates solely by seeds, the species is threatened with extinction. Its seeds are recalcitrant and its germination is slow and uneven, making it necessary to study the maturation process and another propagation technique. Therefore, the objective was to analyze seed maturation, twinning and seedling formation, in line with the development of somatic embryogenesis protocols. For this, seeds were collected during the maturation of different matrices, analyzing morphological, physiological, biochemical and physicochemical characteristics at each stage. For somatic embryogenesis, immature zygotic embryos and seedling stem segments were used as explants in a culture medium supplemented with different growth regulators at each stage, such as dichlorophenoxyacetic acid (2,4-D), 4-amino-3,5,6-trichloropicolinic acid (picloram), 3,5,6-trichloro-2-pyridinyloxyacetic acid (triclopyr), 3,6-dichloro-pyridine-2-carboxylic acid (clopyralyd), abscisic acid (ABA), 1 -naphthaleneacetic acid (ANA), 2-isopentenyladenine (2-iP), in different concentrations. At the end of each stage of somatic embryogenesis, quantitative and qualitative characteristics were analyzed. The time required for the maturation of E. edulis seeds depends on the region and the environmental conditions of each year. The seeds have sufficient reserves and physical-chemical structure that allow their maximum in vitro germination, vigor and formation of normal seedlings still at an immature stage. Embryogenic induction was efficient with the use of picloram (150 µM) or triclopyr (100 µM) in zygotic embryos, and the use of longitudinally sectioned segments and stems, with the wound positioned upwards in the culture medium, of six-month-old seedlings, and picloram (200 µM). The maturation of somatic embryos was effective with the use of ABA (5 µM) for both types of explants. Therefore, it was possible to conclude with the study of seed maturation the best stage for use in in vitro germination and obtaining normal seedlings, with a gain of up to 126 days compared to the use of the last stage of maturation. And the technique of somatic embryogenesis is promising and efficient in the propagation of E. edulis.
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    Ecophysiological aspects of production of Melanoxylon brauna Schott. seedlings
    (Universidade Federal do Espírito Santo, 2025-02-27) Simões, Ingridh Medeiros; Lopes, José Carlos; https://orcid.org/0000-0002-4880-0547 ; http://lattes.cnpq.br/; Alexandre, Rodrigo Sobreira; https://orcid.org/0000-0002-5248-6773 ; http://lattes.cnpq.br/5340049196888351; https://orcid.org/0000-0002-7483-9349; http://lattes.cnpq.br/1675759323773218; Santos, Heloisa Oliveira dos; https://orcid.org/0000-0002-7931-8382 ; http://lattes.cnpq.br/7905575260641836; Maciel, Khetrin Silva; https://orcid.org/0000-0002-8590-7917; http://lattes.cnpq.br/6753673403909545; Pezzopane, José Eduardo Macedo; https://orcid.org/0000-0003-0024-4016 ; http://lattes.cnpq.br/3640768649683482
    The species Melanoxylon brauna Schott. is classified as vulnerable to extinction due to its high level of logging and lack of replanting. Numerous problems are attributed to this species, such as obtaining and viability of seeds, as it is attractive to predators; production of seedlings on a commercial scale in nurseries, due to the substrate used in their propagation, fertilization and excess water, which leads to death. The objective of this work is to analyze the survival and quality of M. brauna seedlings when produced in different soil/substrate compositions, aiming to define the optimal soil/substrate relationship for large-scale seedling production. The soil close to the mother tree (T3) was the one that showed the greatest growth of seedlings after 150 days of setting up the experiment. The orthogonal contrast shows that the underground land (T2) is different for treatments 4; 5; 6 and 7, which have soil close to the matrix in the substrate mixture, which is associated with the activity of the enzymes APX, CAT, POD and SOD. The soil enzymes β-glucosidase and arylsulfatase showed high activity in the organic substrate (T1), followed by the soil close to the parent tree (T3), suggesting that these enzymes are important biochemical markers for evaluating soil quality, however, it is not sufficient to maintaining the survival of seedlings in the organic substrate. For the production of M. brauna seedlings, it is recommended to use 100% soil close to the braúna parent tree. Therefore, it is necessary to elucidate whether the interaction with specific microorganisms, or some metabolite produced by them, by the plant and/or this interaction, are responsible for this survival of M. brauna seedlings
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    Efeito dos tutores de madeira tratada com CCA-C na qualidade da pimenta-do-reino (Piper nigrum L.) cultivada no norte do estado do Espírito Santo
    (Universidade Federal do Espírito Santo, 2023-08-31) Rosa, Arlon Bastos da; Paes, Juarez Benigno; https://orcid.org/0000000347764246; http://lattes.cnpq.br/3454401627877927; https://orcid.org/0000000161335988; http://lattes.cnpq.br/0436776646557918; Alexandre, Rodrigo Sobreira; https://orcid.org/0000000252486773; http://lattes.cnpq.br/5340049196888351; Medeiros Neto, Pedro Nicó de; https://orcid.org/0000-0001-5647-0050; http://lattes.cnpq.br/6441001734932075; Baraúna, Edy Eime Pereira; https://orcid.org/0000-0001-5239-9592; http://lattes.cnpq.br/3160416445274012; Lopez, Yonny Martinez
    The objective of the research was to analyze the effects of using wooden stakes treated with chromated copper arsenate, type C (CCA-C) on the quality of black pepper (Piper nigrum L.) cultivated in the north of the State of Espírito Santo. To evaluate the concentration of copper, chromium and arsenic elements, components of CCA-C, samples were collected from underground roots, green and mature fruits of plants aged 5; 7 and 13 years, plus the control (5-year-old plants, untreated wooden tutor). For the quantification of the elements, the material was dried, crushed and digested in a 2N hydrochloric acid (HCl) solution, and submitted to chemical analysis in mass spectrometry with inductively coupled plasma (ICP-MS/MS), using helium (He), as a collision gas. The results indicated that copper was found in higher concentrations in plants, being more adsorbed by underground roots, with a decrease in green and ripe fruits. Chromium behaved similarly to copper, and arsenic did not show differences between the analyzed ages, being with a concentration lower than that allowed by the Resolution of the Collegiate Board - RDC 26, June 2nd, 2015, of the Brazilian Health Ministry. For plants aged 5 and 7 years (treated tutors) copper concentration was observed above the maximum allowed. However, copper concentrations in mature fruits from plants aged 13 years were within acceptable values. Thus, fruits of different ages and plant staking must be mixed and homogenized in order to obtain a good quality product that can be consumed without major consequences to human health.
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    FISIOLOGIA DE SEMENTES E METABOLISMO ANTIOXIDATIVO NA EMERGÊNCIA DE Lecythispisonis CAMBESS.: INFERÊNCIAS SOBRE TEMPERATURA ÓTIMA E APTIDÃO CLIMÁTICA PARA A SEMEADURA
    (Universidade Federal do Espírito Santo, 2022-02-24) Rosa, Thuanny Lins Monteiro; Alexandre, Rodrigo Sobreira; https://orcid.org/0000000252486773; http://lattes.cnpq.br/5340049196888351; https://orcid.org/0000000169796914; http://lattes.cnpq.br/; Ferreira, Adesio; https://orcid.org/0000000270001725; http://lattes.cnpq.br/5400370038397801; Mengarda, Liana Hilda Golin; https://orcid.org/; http://lattes.cnpq.br/; Freitas, Allan Rocha de; https://orcid.org/; http://lattes.cnpq.br/; Maciel, Khetrin Silva
    The climate changes registered in the last decades have triggered a severe loss of biodiversity. High temperatures induce a high reproductive effort of trees that is not translated into high production of viable seeds. The temperature is one of the most i
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    PROPAGAÇÃO DE Paratecoma peroba POR MINIESTAQUIA
    (Universidade Federal do Espírito Santo, 2020-02-28) Araujo, Emanuel Franca; Goncalves, Elzimar de Oliveira; https://orcid.org/0000000176752493; http://lattes.cnpq.br/4127505406039950; https://orcid.org/0000000190906006; http://lattes.cnpq.br/; Alexandre, Rodrigo Sobreira; https://orcid.org/0000000252486773; http://lattes.cnpq.br/5340049196888351; Klippel, Valeria Hollunder; https://orcid.org/; http://lattes.cnpq.br/; Xavier, Talita Miranda Teixeira; https://orcid.org/; http://lattes.cnpq.br/
    The forest species Paratecoma peroba (Record and Mell) Kuhlm, belongs to the family Bignoniaceae, being native to the Atlantic Forest, presenting relevant ecological and economic interest. It has a restricted area of natural occurrence within the coastal