Análise de cocaína e seus metabólitos em fios de cabelo por MALDI-TOF MS
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Data
2023-12-12
Autores
Donateli, Patrícia Oliveira
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Universidade Federal do Espírito Santo
Resumo
Hair can provide chemical information about drug use, which generates great interest in forensic sciences. A technique that has shown promising results for the detection of drugs in this study matrix is the matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), in which it is possible to achieve, in a single shot, the detection of drugs of abuse, even if a long time has elapsed since the use of the illicit compound. In this study, the MALDI ionization source was used to detect drugs and metabolites in the hair strands of drug users and non-drug users. For this, a method was developed with an initial evaluation of the most efficient MALDI matrix for the ionization of the monitored analytes. Among the evaluated matrices (2,5-dihydroxybenzoic acid (DHB) and α-cyano-4-hydroxycinnamic acid (CHCA)), CHCA was chosen, and among its tested concentrations (from 1.5 to 10 mg mL-1). Subsequently, the results were compared with gas chromatography-mass spectrometry analyses, where a good relationship was observed between the responses of both methods. In addition, the effect of the pre-analysis (hair washing) and its influence on the detection of the monitored compounds were evaluated, in addition to the evaluation of the effect of hair lightening on hair fortified with a drug and analyzed with the proposed method. The results show little influence on the preanalysis stage and confirm the reduction in drug detection in hair submitted to bleaching. In this sense, the results add information that asserts the applicability of the method for rapid detection of drugs and metabolites in hair.
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Química forense , Drogas de abuso , MALDI