Relação das proteínas da via de estresse oxidativo com elementos traços, hábitos de vida e prognóstico em pacientes com carcinoma epidermóide de cavidade oral
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Data
2019-05-09
Autores
Assis, Aricia Leone Evangelista Monteiro de
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Universidade Federal do Espírito Santo
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Introduction: Squamous cell carcinoma of the oral cavity is a multifactorial disease with a strong association with the use of tobacco, which has metal and metalloid constituents responsible for the generation of reactive oxygen species (ROS). The loss of balance between production and elimination of ROS is called oxidative stress and can lead to damage in DNA, RNA, lipids and proteins. In addition to DNA fragmentation, ROS can cause the DNA repair system to malfunction, contributing to the development of diseases, such as cancer. However, the cells have antioxidant mechanisms for the maintenance of oxidative homeostasis and repair if the damage occurs in the DNA. Thus, the main objective of this study was to understand the mechanisms and cellular interactions of oxidative stress and repair enzymes and their association with epidemiological characteristics and elements present in the tumor samples. Casuistry and methods: In this context, the proposal sought to study tumors of 78 patients with oral cancer related to the biological interactions of proteins involved in oxidative stress (SOD-1 and Trx) and repair (Ref-1 and OGG1 / 2), besides evaluating its associations with epidemiological and prognostic characteristics and the presence of chemical elements in the tumor tissues. In order to do so, we analyzed the expression of the proteins by means of immunohistochemistry, elemental characterization by μ-XRF and verified the associations with epidemiological and prognostic characteristics of the patients. Results: The analysis of the metal and lifestyle protein interactions showed that the smoking habit is associated with increased expression of SOD-1, OGG1 / 2 and Trx, the chromium element was associated with Re-1 and Trx, nickel with Ref-1 and magnesium with OGG 1/2. It was also possible to observe a strong protein-protein interaction of OGG 1/2 with SOD-1. On the other hand, the results more focused on the prognosis of the patient, it was possible to verify that the elements of chlorine and chromium were related to the recurrence of the tumor. Perineural and vascular invasion were important indicators for aggression and tumor prognosis, and expression of SOD-1, Trx and Ref-1 showed a prognostic relationship with tumor growth. The alcoholic habit and vascular invasion had an impact on disease-free and disease-specific survival. Conclusions:The data lead to a better understanding of the performanceof the chemical elements, absorbed through the smokinghabit, in the possible modulat ion of the expression ofproteins of oxidative stress and DNA repair and itsassoc iation with the clinicopathological characteristics andPrognostic of the patients.
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Cancer oral , Estresse oxidativo , Metais , Enzimas de reparo , Prognóstico , Oral cancer , Oxidative stress , Metals , Repair enzymes , Prognosis