Doutorado em Química

URI Permanente para esta coleção

http://repositorio.ufes.br/handle/10/17501

Nível: início
Ano de início: 2014
Conceito atual na CAPES: 5
Ato normativo: Homologação da 85ª Reunião do CTC-ES, Parecer CNE/CES nº 163/2005.
Processo nº 23001.000081/2005-56 do Ministério da Educação.
Publicado no DOU 28/07/2005, seção 1, página 11)
Periodicidade de seleção: Anual
Área(s) de concentração: Química
Url do curso: https://quimica.vitoria.ufes.br/pt-br/pos-graduacao/PPGQ/detalhes-do-curso?id=956/a>

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Navegando Doutorado em Química por Assunto "5-HMF"

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    Estudo de méis de abelha com ferrão (Apis mellifera) e sem ferrão por ressonância magnética nuclear
    (Universidade Federal do Espírito Santo, 2025-10-01) Vieira, Thays Cardoso Valim; Cunha Neto, Álvaro; https://orcid.org/0000-0002-1814-6214; http://lattes.cnpq.br/7448379486432052; https://orcid.org/0000-0002-7577-6458; http://lattes.cnpq.br/4042494798216516; Berrué, Fabrice; https://orcid.org/0000-0003-2389-4388; Garrido, Bruno Carius; https://orcid.org/0000-0001-5068-9879; http://lattes.cnpq.br/6241191567638205; Ferreira, Rafael de Queiroz; https://orcid.org/0000-0002-5190-8508; http://lattes.cnpq.br/5053247764430323 ; Filgueiras, Paulo Roberto; https://orcid.org/0000-0003-2617-1601; http://lattes.cnpq.br/1907915547207861
    The growing demand on functional or nutraceutical foods shows that people are increasingly seeking healthier diets, with a preference for natural products that, in addition to their nutritional benefits, also provide antioxidant, antitumor, anti-inflammatory, and other bioactive properties. In this context, studying foods, from their chemical composition to quality control, is essential to ensure both safety and authenticity prior to commercialization. Regulatory agencies such as Anvisa (Brazilian Health Regulatory Agency) in Brazil and the FDA (U.S. Food and Drug Administration) in the United States are continuously developing control methods to assess food composition, detect adulterants, verify shelf life, and even determine geographical origin. Honey, one of the most widely consumed functional foods worldwide, stands out not only for its nutritional benefits and high commercial value but also for the increasing incidence of adulteration, which highlights the need for strict quality parameters. Current protocols for honey analysis largely rely on chromatographic, spectrophotometric, and colorimetric techniques. Based on these considerations, the present work aimed to investigate the potential of nuclear magnetic resonance (NMR) spectroscopy as an analytical tool and to propose it as an alternative for honey quality control. NMR, as a spectroscopic technique capable of providing both qualitative and quantitative information in a single run, was applied to honey samples from sting (Apis mellifera) and stingless bees collected in Brazil and Canada. Critical instrumental parameters, such as relaxation time (T1) and receiver gain (RG), were evaluated, and their direct influence on the robustness, reproducibility, and accuracy of the results was demonstrated. The effect of relaxation times on analytes was studied using three spectrometers (Varian 400 MHz, Bruker 500 and 700 MHz) equipped with different probes. Furthermore, a method was validated for the simultaneous quantification of glucose, fructose, maltose, sucrose, and 5-hydroxymethylfurfural (5-HMF) in honey. The method exhibited excellent performance in terms of linearity and precision, along with satisfactory detection and quantification limits and accuracy. It was successfully applied to 60 honey samples from different bee species, showing that despite the equimolar response characteristic of the technique, calibration curves can be essential for analytes with long T1 values. The results also demonstrated the effectiveness of the methodology in distinguishing samples according to bee species and geographical origin, confirming NMR as a robust alternative to conventional honey analysis protocols. Finally, the annexed work reflects the importance of international collaborations, which expand access to diverse samples, enable the use of different equipment, promote the exchange of technical expertise, and ultimately contribute to the improvement of analytical protocols and the researcher’s academic development.