Ciência e Tecnologia de Alimentos

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http://repositorio.ufes.br/handle/10/17468
Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos

Centro: CCAE
Telefone: (28) 3552 8719
URL do programa: https://cienciaetecnologiadealimentos.ufes.br/pt-br/pos-graduacao/PPGCTA

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Navegando Ciência e Tecnologia de Alimentos por Assunto "Ácido clorogênico"

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    Efeito do extrato aquoso de café verde (Coffea canephora) na saúde intestinal de animais alimentados com dieta hipercalórica e hiperlipídica
    (Universidade Federal do Espírito Santo, 2024-09-29) Meneguelli, Natália Alves de Souza; Trivilin, Leonardo Oliveira ; https://orcid.org/0000-0002-3039-3269; http://lattes.cnpq.br/5723897284006298; Grancieri, Mariana; https://orcid.org/0000-0001-8888-5496; http://lattes.cnpq.br/6646062052507980; Costa, André Gustavo Vasconcelos ; https://orcid.org/0000-0002-2393-6384; http://lattes.cnpq.br/5406130298857736; https://orcid.org/0009-0007-3247-3004; http://lattes.cnpq.br/9189123775450715; Sant' Ana, Cíntia Tomaz ; https://orcid.org/0000-0002-1385-9274; http://lattes.cnpq.br/5267705234203118; Cintra, Dennys Esper Corrêa ; https://orcid.org/0000-0002-7954-5630; http://lattes.cnpq.br/2329875440704117
    Inadequate eating habits, such as excessive caloric intake and diets high in saturated fats, are associated with the development of obesity and intestinal alterations. Green coffee is rich in bioactive antioxidant compounds, which may assist in obesity management. However, its effects on intestinal health, particularly in the context of obesity, remain unclear. This study aimed to evaluate the effect of aqueous green coffee extract on intestinal health in an animal model of obesity. A total of 32 adult Wistar rats were divided into four experimental groups (n=8): CT – negative control (commercial control diet without green coffee extract supplementation), HF – positive control (obesity-induced without green coffee extract), HFP – prevention experimental group (obesity-induced with extract supplementation from the first experimental week), and HFT – treatment experimental group (obesity-induced with extract supplementation starting from the 8th week). Obesity was induced over eight weeks through a hypercaloric and high-fat diet (HF) diet. At the end of the 16th week, the animals were euthanized. Colon tissue samples were collected for analysis of oxidative markers (catalase, superoxide dismutase - SOD, and nitric oxide - NO), morphometric parameters and junction protein. Cecal and colonic contents were used to analyze pH, short-chain fatty acids (SCFAs), and immunoglobulin A (IgA). Food intake was monitored daily, and body weight was measured weekly. For statistical analysis, at test was applied between CT and HF groups, while ANOVA followed by Tukey’s post hoc test was used for HF, HFP, and HFT groups, adopting a 5% significance level. Compared to the CT group, the HF group exhibited significantly higher means (p<0.05) for feed and caloric efficiency coefficients, weight gain, and murinometric parameters, confirming the induction of obesity. A significant reduction (p<0.05) in intestinal pH, NO, SOD, morphometric parameters, claudin, and occludin was also observed. However, supplementation with the green coffee extract did not influence parameters related to food intake or murinometric measurements. On the other hand, in the HFP group, the extract prevented mucosal alterations and the reduction of junction proteins, whereas in the HFT group, it promoted a reduction in intestinal pH, an increase in butyrate and acetate levels, morphometric recovery, and increased claudin and occludin quantification. These findings demonstrate the beneficial effects of aqueous green coffee extract on intestinal health, highlighting its potential as a strategy for preventing and treating intestinal alterations induced by a high-fat diet
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    Obtenção do extrato de café verde conilon (Coffea canephora) e seus efeitos na resposta pós-prandial de incretinas e na homeostase da glicose
    (Universidade Federal do Espírito Santo, 2026-02-25) Silva, Thalia Gomes; Grancieri, Mariana ; https://orcid.org/0000-0001-8888-5496; https://lattes.cnpq.br/6646062052507980; Sant' Ana, Cíntia Tomaz; https://orcid.org/0000-0002-1385-9274; https://lattes.cnpq.br/5267705234203118; Costa, André Gustavo Vasconcelos ; https://orcid.org/0000-0002-2393-6384; https://lattes.cnpq.br/5406130298857736; https://orcid.org/0009-0007-2824-4778; https://lattes.cnpq.br/5338614493677967; Costa, Neuza Maria Brunoro ; https://orcid.org/0000-0002-4967-9937; https://lattes.cnpq.br/9592871700382838; Bressan, Josefina ; https://orcid.org/0000-0002-4993-9436; https://lattes.cnpq.br/6723886773861125
    Coffee is one of the most consumed beverages worldwide and has been extensively investigated due to its potential beneficial health effects, mainly attributed to its phenolic and bioactive compounds. Robusta coffee (Coffea canephora) presents higher levels of these compounds in the form of green beans, since the roasting process promotes significant losses of these compounds. In this context, the present study aimed to evaluate different extraction methods of green robusta coffee and investigate the effects of consumption on glycemic, hormonal, and subjective appetite parameters in eutrophic or obese individuals. The different extraction methods were compared in relation to the content of total phenolic compounds, antioxidant capacity, and content of bioactive compounds. According to the results found, among the methods evaluated, conventional infusion for 10 minutes at 90 ºC presented higher concentrations of compounds of interest and high antioxidant capacity when compared to the other methods evaluated, thus being chosen for use in the acute clinical trial. A randomized, single-blind, crossover study was conducted with a one-week washout period between tests. Volunteers, divided into two groups—one of eutrophic individuals (n=10) and the other of obese individuals (n=9)—consumed a control syrup containing 75g of glucose and 250 mL of water, and a syrup containing 55 mL of green coffee extract, 195 mL of water, and 75g of glucose. Blood samples were collected at 0 (fasting), 15, 30, 60, and 120 minutes, simulating an oral glucose tolerance test (OGTT). At the same collection times, volunteers also answered questionnaires regarding subjective appetite parameters using a visual analog scale, allowing for an assessment of subjective appetite perceptions throughout the postprandial period. Postprandial glycemic and hormonal responses showed expected physiological behavior after glucose ingestion, with an initial increase in blood glucose followed by increased insulin secretion and the release of the incretins GIP and GLP-1. However, no significant differences were observed in blood glucose and GIP in the total and incremental areas under the curve (AUC) (p>0.05). However, ingestion of glucose syrup containing green coffee extract significantly reduced the total and incremental AUC of insulin (p<0.05), without altering blood glucose, suggesting greater metabolic efficiency and possible improvement in insulin sensitivity. A reduction in the incremental AUC of GLP-1 (p<0.05) and an increase in the tAUC of glucagon (p<0.05) were also observed. Regarding subjective appetite parameters, assessed using the visual analog scale, no significant diferences were observed. The results suggest that, although green coffee extract did not modulate postprandial glycemia, it was able to alter hormonal responses related to glycemic homeostasis, reinforcing the potential modulating effect of acute consumption of the extract on glucose metabolism