Doutorado em Agronomia
URI Permanente para esta coleção
Nível: Doutorado Acadêmico
Ano de início: 2010
Conceito atual na CAPES: 4
Ato normativo: Doutorado - Reconhecido Port. MEC 609, de 14/03/2019, DOU 18/03/2019, parecer 487/2018).
Periodicidade de seleção: Semestral
Área(s) de concentração: Produção Vegetal
Url do curso: https://producaovegetal.ufes.br/pt-br/pos-graduacao/PPGA/detalhes-do-curso?id=1440
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- ItemMétodos de manejo para Liriomyza sativae Blanchard, 1938 (Diptera: Agromyzidae(Universidade Federal do Espírito Santo, 2021-08-27) Tamashiro, Luiza Akemi Gonçalves; Pratissoli, Dirceu; https://orcid.org/0000-0003-4485-1491; http://lattes.cnpq.br/4015405807686646; https://orcid.org/0000-0002-4853-0006; http://lattes.cnpq.br/9884791626282822; Menini, Luciano; https://orcid.org/0000000336565428; http://lattes.cnpq.br/2646344913961349; Bueno, Regiane Cristina Oliveira de Freitas; https://orcid.org/0000-0002-6210-8231; http://lattes.cnpq.br/9921033869437455; Santos Junior, Hugo José Gonçalves dos; https://orcid.org/0000000267806610; http://lattes.cnpq.br/8499663924650322; Araujo Junior, Luis Moreira de; http://lattes.cnpq.br/1398623308889710Leafminer (Liriomyza spp.) (Diptera: Agromyzidae) are insects distributed throughout the world, have a polyphagous habit, feed on a wide variety of hosts belonging to the Fabaceae, Curcubitaceae and Solanaceae family. They have developmental behavior in the leaf mesophyll, causing the formation of galleries in the leaves. The injuries can result in a reduction in the photosynthetic rate of the plant, drying and loss of leaves and, consequently, a reduction in fruit production. The application of synthetic insecticides is the main control strategy for these pest species. However, the excessive use of these products causes problems such as human and environmental contamination and genotype resistance. Thus, research related to more sustainable management strategies is of great relevance. The objective of this research was to develop alternative control strategies through studies with (1) attractive substances for the capture of adults of L. sativae (2) insecticidal and deterrent effect of essential oils and major compounds on different phases of L. sativae, 3) evaluate the compatibility of entomopathogenic nematodes with essential oils, fixed oils and major compounds in the infectivity of L. sativae larvae. From the results obtained, it was found that: (1) formulations containing banana vinegar (10%), molasses (10%) and citric acid (0.5%) and invert sugar (3%), molasses (5%), hydrolyzed protein (0.5%) and citric acid (0.5%) captured the largest number of adults of L. sativae during the period of 12 and 14 days, respectively, compared to the other formulations, (2) citronella essential oil showed the highest toxicity in L. sativae larvae and neem oil the highest deterrence in adults compared to other oils, (3) essential oils, fixed oils and major compounds caused mortality, reduced number of lateral body strokes and infectivity in L. sativae larvae, with emphasis on eucalyptus, ginger and rosemary oils. The evaluated strategies can be used to help reduce the population of L. sativae in integrated management programs.
- ItemMultiplicação de Tetrastichus howardi (OLLIFF,1893) (Hymenoptera: Eulophidae) em hospedeiros anternativos(Universidade Federal do Espírito Santo, 2022-07-13) Olive, Emily Lopes; Zago, Hugo Bolsoni; https://orcid.org/0000000319753590; http://lattes.cnpq.br/6021586329353959; https://orcid.org/0000-0003-1537-186X; http://lattes.cnpq.br/9523118113798215; Fernandes, Kenner Morais; https://orcid.org/; http://lattes.cnpq.br/4670384069546459; Junior, Hugo Jose Goncalves dos Santos; https://orcid.org/0000000267806610; http://lattes.cnpq.br/8499663924650322; Araujo Junior, Luis Moreira de; https://orcid.org/0000-0003-3354-9465; http://lattes.cnpq.br/1398623308889710; Zuim, Vitor; http://orcid.org/0000-0001-9702-8568; http://lattes.cnpq.br/6046477852251944Tetrastichus howardi (Olliff, 1893) (Hymenoptera: Eulophidae) is a parasitoid of several insect pests in Brazil. However, it is necessary to ensure quality control in the process of successive multiplication on the hosts under laboratory conditions. Thus, the biological and morphological characteristics of T. howardi multiplied in two alternative hosts, Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) and Tenebrio molitor (Linnaeus, 1758) (Coleoptera: Tenebrionidae) were evaluated. In addition, we identified the parasitoid via molecular taxonomy and determined the metabolic profile of the number of T. howardi descendants from successive multiplications in alternative hosts. In S. frugiperda the parasitoid managed to maintain a higher emergence than in T. molitor, where it tended to decrease the number of offspring until non-emergence after the 10th generation. The cycle length tended to increase in more advanced generations, but the sex ratio was above 60% in both hosts. The length of the tibia and wing did not vary significantly between generations, but parasitoids from pupae of S. frugiperda were larger than those of T. molitor. The obtained DNA sequences had efficiency superior to 99%, through the Barcode of Life Data System. The analysis by gas-chromatography with mass spectometry allowed the quantification of 118 main metabolites of T. howardi. There is potential to use S. frugiperda pupae up to the 19th generation of T. howardi and T. molitor pupae up to the third generation without needing to reinvigorate. The DNA sequences will contribute to the purity of laboratory creations, in addition to providing the molecular database of T. howardi in the neotropical region. The pupae of S. frugiperda and T. molitor are suitable for mass multiplication of T. howardi. However, T. molitor pupae should not be used for more than three successive generations, as there are losses in the quality of the parasitoid.
- ItemTransferibilidade de marcadores microssatélites e adaptação de Trichogramma Pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae) sob estresse de temperatura(Universidade Federal do Espírito Santo, 2018-02-28) Araujo Junior, Luis Moreira de; Pratissoli, Dirceu; Zago, Hugo Bolsoni; Ferreira, Marcia Flores da Silva