Doenças Infecciosas

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http://repositorio.ufes.br/handle/10/17577
Programa de Pós-Graduação em Doenças Infecciosas

Centro: CCS
Telefone: (27) 3335 7504
URL do programa: http://doencasinfecciosas.ufes.br/pos-graduacao/PPGDI

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Navegando Doenças Infecciosas por Autor "Antunes, João Marcelo Azevedo de Paula"

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    Pesquisa de anticorpos anti-PGL-I em pacientes infectados pelo HIV em área endêmica para hanseníase
    (Universidade Federal do Espírito Santo, 2015-02-26) Madureira, Brunela Pitanga Ramos; Deps, Patrícia Duarte; Falqueto, Aloisio; Palaci, Moises; Antunes, João Marcelo Azevedo de Paula; Hadad, David Jamil
    The present study investigated subclinical Mycobacterium leprae infection in human immunodeficiency virus (HIV) infected or uninfected individuals by measuring antibodies against M. leprae phenolic glycolipid I (PGL-I). It also sought to establish whether the serology results correlated with the HIV-infected participants’ state of immunosuppression. This was a cross-sectional study that analysed the anti-PGL-I immunoglobulin M antibody levels in 350 HIV-infected and 350 non-HIV-infected individuals residing in an area endemic for the two investigated conditions. The possible correlation between the state of immunosuppression of the HIV-infected participants (CD4+ cell count, viral load and the use or not of antiretroviral therapy) and anti-PGL-I seropositivity was assessed. Approximately 6% (21/350) of the HIVinfected and 29.1% (102/350) of the non-HIV-infected participants were anti-PGL-I antibody seropositive. The number of anti-PGL-I antibody seropositive individuals was approximately five-fold higher in the non-HIV-infected group, compared with the HIV-infected group. There was no significant correlation between the state of immunosuppression of the HIV-infected participants and the anti-PGL-I serology results. The HIV-infected individuals exhibited less anti-PGL-I antibody production relative to the control group, which may indicate a lower rate of subclinical M. leprae infection and/or lower specific production of this antibody. B cell dysregulation might cause the low anti-PGL-I antibody production in HIV-infected individuals. The patients’ state of immunosuppression did not correlate with the anti-PGL-I serology results.
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    Pesquisa de Mycobacterium leprae em tatus selvagens da espécie Dasypus novemcinctus no Estado do Espírito Santo
    (Universidade Federal do Espírito Santo, 2007-11-05) Antunes, João Marcelo Azevedo de Paula; Zanini, Marcos Santos; Deps, Patrícia Duarte; Virmond, Marcos da Cunha Lopes; Silva, Paulo César Amaral Ribeiro da
    Introduction: Mycobacterium leprae, was the first agent to be linked to an infectious disease, and leprosy still today an enigmatic disease which is not fully understood. Multibacillary patients are thought to be the main source of M. leprae. Identification of M. leprae is difficult, partly due to the inability of the bacillus to grow in vitro. The natural transmission among nine-banded armadillos in the southern parts of the United States has been described since 1975. This discovery suggested the possibility that the nine-banded armadillo plays a role in the transmission of human leprosy. The search of M. leprae sources is the main point of strategy for leprosy elimination, such as multibacillary patients and environmental sources. It was realized a prevalence study in 69 nine-banded armadillos, Dasypus novemcinctus, from state of Espírito Santo, Brazil, during 2004 to 2005. Objectives: Assess the M. leprae infection in armadillos from D. novemcinctus from state of Espírito Santo through the clinical signs of leprosy found in the necropsy and PCR technique using two set of primers, ML1/ML2 and 18 kDa, such as compare the set of primers. Methods: After anesthesia, the armadillos were weighed, sex was determined, clinical exam was made and samples were collected for PCR. Tissue samples (liver, spleen, brain and lymph nodes) from 65 nine-banded armadillos were collected to looking for natural infection of M. leprae by PCR technique. Results and Conclusion: 95% of the armadillos had at least one clinical sign for leprosy in wild armadillos. Four (6%) animals were positive in conventional PCR. Two animals that were positive in conventional PCR remained positive in Real Time PCR, and confirmed by sequencing. The best method to detect M. leprae was the PCR using the ML1/ML2 set of primers. These results suggest the infection acquired naturally in D. novemcinctus from state of Espírito Santo, Brazil.