Ciências Veterinárias

URI Permanente desta comunidade

http://repositorio.ufes.br/handle/10/17470
Programa de Pós-Graduação em Ciências Veterinárias

Centro: CCAE
Telefone: (28) 3552 8983
URL do programa: http://www.cienciasveterinarias.ufes.br/pos-graduacao/PPGCV

Navegar

Navegando Ciências Veterinárias por Autor "Andrade, Gracielle Ferreira"

Agora exibindo 1 - 1 de 1
  • Nenhuma Miniatura disponível
    Item
    Desenvolvimento farmacotécnico e atividade antimicrobiana e antioxidante de gel de carbômero contendo extrato de casca de Punica granatum para uso em lesões cutâneas em cães
    (Universidade Federal do Espírito Santo, 2021-08-30) Silva, Thaís Martins da; Konishi, Janaina Cecilia Oliveira Villanova; https://orcid.org/0000000161661724; http://lattes.cnpq.br/4226187656439843; http://lattes.cnpq.br/4355128335306705; Andrade, Gracielle Ferreira; https://orcid.org/0000-0001-9038-1254; http://lattes.cnpq.br/9137739604308170; Resende, Juliana Alves; https://orcid.org/0000000254763754; http://lattes.cnpq.br/8223821041049149; Giuberti, Cristiane dos Santos; https://orcid.org/0000000205608731; http://lattes.cnpq.br/3644255863698492
    The peel is one of the biowaste of pomegranate’s fruits formed after the extract of the juice, with the potential to be converted into products with added value for many applications, including the pharmaceutical. The purpose of this present work was to prepare a gel containing dry total extract from pomegranate peel (named EBSCR) with purpose on the use of handling of skin lesions in veterinary’s pharmacotherapy of dogs, with the intention of substantiating the interest of value’s added on the biowastes of pomegranate. At first, was carried out the determination of the content of total phenols and the total antioxidant activity on the extract, being defined as CE50 (effective concentration to suppress 50%) e a CE90 (effective concentration to suppress 90%), using BHT (butylhydroxytoluene) as a pattern. After chemical characterization, was prepared formulations of carbomer’s gel with 1% p/p containing the EBSCR with different concentrations (1.25, 2.5, and 3.75% p/p), called FG1, FG2, and FG3, then was submitted to activity’s research antimicrobial through the disc-diffusion method. In order to do this, was used strains of Staphylococcus aureus (ATCC 25.923), Staphylococcus epidermidis (ATCC 12228), Escherichia coli (ATCC 25.922) e Candida albicans (ATCC 24.433). After of being elected the formulation with the best concentration of the extract for the continuation of the research was prepared 1.5 kg of base gel e in 1 kg was incorporated the extract in the concentration elected for the research (FG2; 2.5% p/p). The product was fractionated, and the samples were stored under refrigeration (5 ± 2º C) and at a temperature (27 ± 2º C), determined on executions’ period of the research to the evaluation of preliminary stability. The physicochemical tests were carried out on FG2 for 90 days: aspect, pH, thermal and mechanical stress, texture profile, contents of all phenols, microbiological quality, and antimicrobial activity maintenance. The research of the constituents on the extract by UHPLC suggests the existence of phenols composites of interest to the antimicrobial and healing activity in the extract. The content of total phenols found was of 61.92 g/EAG and the antioxidant activity total determined for a solution with 40 µg.mL-1 was 93.8% with the values of the minimum effective concentrations (EC) to reduce the DPPH to 50% and 90% of 14.44 µg.mL-1 and 39.82 µg.mL-1 , respectively. The incorporation of the EBSCR to 2.5% on the base’s gel created an opaque gel, with intense yellow color, with a pH of 6.10. During the time, the pH, texture profile, and phenols content decreased, being the biggest alterations noted on the products kept out of the refrigerator. At the end of 30 days, the reduction on the phenols content total in the formulation was lower than 9%, only to the sample stored under refrigeration. Concerning about the microbiological quality, the formulations were in according to the count of mesophiles microorganism total and absence of pathogenic microorganisms, without changes for 90 days. The antimicrobial effectiveness was kept during the study time. With the results can infer that the formulation FG2, prepared as of carbomer base’s gel to 1% p/p containing EBSCR at 2.5% p/p, stored during 30 days under refrigeration can be used to the intended purpose, being useful to add value to biowastes of pomegranate.