Agronomia

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http://repositorio.ufes.br/handle/10/17348
Programa de Pós-Graduação em Agronomia

Centro: CCAE
Telefone: (28) 3552 8983
URL do programa: https://producaovegetal.ufes.br/pt-br/pos-graduacao/PPGA/

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    Análises fenotípicas e moleculares na identificação de SNPs associados aos conteúdos de proteínas total e de reserva de soja [Glycine max (L.) Merril]
    (Universidade Federal do Espírito Santo, 2020-02-28) Lorenzoni, Rodrigo Monte; Soares, Tais Cristina Bastos; https://orcid.org/0000000163567993; http://lattes.cnpq.br/6580031598802359; https://orcid.org/0000000268337692; http://lattes.cnpq.br/0777442719925154; Costa, Maximiller Dal Bianco Lamas; https://orcid.org/0000-0002-7001-4841; http://lattes.cnpq.br/5843464065448139; Meira, Eduardo Frizzera; https://orcid.org/0000-0002-0210-4319; http://lattes.cnpq.br/9651828257315064; Zucoloto, Moises; https://orcid.org/0000000305394750; http://lattes.cnpq.br/9552365183163692; Piovesan, Newton Deniz; http://lattes.cnpq.br/6391356469775029; 0000-0003-0249-8562
    Storage proteins correspond to approximately 70% of the total protein content in soybean seeds and the proportions between its main fractions (7S and 11S) influence nutritional quality. This characteristic affects the soy-based food industry for human consumption and also the use of bran in animal feed. Due to this dynamic among the stotage proteins, breeding programs have been developed in order to modulate the nutritional quality of soybeans. In order to contribute to the identification of useful markers for assisted selection, the objective of this study was to estimate the effect of SNPs markers in a population of RILs derived from the crossing between two elite pre-selected genotypes of the ‘breeding program for soybean quality' from BIOAGRO-UFV (PMQS-80 and PMQS-12) aimed at increasing protein content and nutritional quality, and identifying candidate genes present in the region proximal to the validated marker and that have an effect on the characteristics of interest. For this, 271 F5-derived RILs were tested in field trials in Capinópolis and Viçosa-MG, in the 2017/18 and 2018/19. This material was analyzed for characteristics: total protein content in soybean seeds, 26 content of storage proteins 7S and 11S and their respective subunits. Subsequently, the individuals were genotyped and an associative analysis was carried out between the morphological and molecular characters. The average total protein content in the Capinópolis environments was 48.947 and 47.651%, being higher than those observed in Viçosa, which were 45.078 and 45.629%. The same pattern was observed for reserve proteins with 40.225 and 38.019% in Capinópolis and 33.091 and 31.010% in Viçosa. As a percentage, the PTN_Res / PT ratio was higher in Capinópolis than Viçosa. Heritabilities above 70% were observed for PT, while for PTN_Res the values were between 50 and 60%. Correlations between PT and the storage proteins and their subunits were verified. PT showed a positive correlation with all variables, while the subunits of the 7S fraction showed a positive correlation between them and negative with the subunits of the 11S fraction, the reverse was also verified. The correlation between 7S and 11S proteins was close to 0, but both showed positive correlations with the sum of 7S + 11S. In the associative analysis, SNP 190 was associated in the four environments for the variables PT, PTN_Res, acidic and basic subunits, 11S and 7S + 11S protein and presented the maximum explanation of 31.042% of the phenotypic variation in CAP 2 cultivation, as well as the largest additive effects for both PT and PTN_Res. Finally, to search for DEGs related to protein accumulation in soybean seeds, the readings of the PMQS80 and PMQS12 readings were mapped against the reference genome and then four DEGs were selected within the region close to the SNP190, among them the Glyma.20g084500 which is related to the development of the endosperm and, therefore, may be associated with the accumulation of proteins in soybean seeds. In view of all the observed results, SNP190 is a potential marker to be used in assisted selection in soybean breeding programs, since it has stability and, the protein content and the subunit composition of the storage proteins are strongly influenced by environmental factors and pleiotrophic effects