Ciências Odontológicas

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http://repositorio.ufes.br/handle/10/17573
Programa de Pós-Graduação em Ciências Odontológicas

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URL do programa: http://www.odontologia.ufes.br/pt-br/pt-br/pos-graduacao/PPGCienciasOdontologicas

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Navegando Ciências Odontológicas por Autor "Campagnaro, Bianca Prandi"

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    Efeito da fotobiomodulação na peridontite experimental de camundongos hipercolesterolêmicos Apoe knockout
    (Universidade Federal do Espírito Santo, 2023-08-21) Ribeiro, Isadora Martins; Campagnaro, Bianca Prandi; https://orcid.org/0000-0002-9023-4892; http://lattes.cnpq.br/6847063606394701; Meyrelles, Silvana dos Santos ; https://orcid.org/0000-0003-0167-4093 ; http://lattes.cnpq.br/7731215198101947; https://orcid.org/0000-0003-1008-1724; http://lattes.cnpq.br/4926826167047170; Ramos, Umberto Demoner; https://orcid.org/0000-0002-3759-1364; http://lattes.cnpq.br/7171760362653486; Tavares, Marcella Porto; https://orcid.org/0000-0003-4234-9721; http://lattes.cnpq.br/0850829266775500; Silva, Daniela Nascimento; https://orcid.org/0000-0003-0145-2247; http://lattes.cnpq.br/3613001852759565
    Photobiomodulation therapy (PBM) has attracted special attention and can lead to positive physiological responses or inhibitory biological effects. PBM has been studied mainly in the treatment of Periodontal Disease (PD), which is one of the most common inflammatory conditions. The periodontitis-systemic disease relationship constitutes an important part of clinical periodontal research. Cardiovascular diseases, which are an example of diseases worsened by PD, can be caused by hypercholesterolemia, which has been proposed as a possible link between chronic periodontal inflammation and atherosclerosis. Therefore, this study seeks to evaluate the effects of photobiomodulation by infrared irradiation (NIR-PBM) on oxidative stress and inflammatory activity in the knockout animal model (ApoE-/-) with PD caused by ligation. ApoE-/- mice aged 16 weeks and 25-30 g were used. Induction of periodontitis by ligation was performed over a period of four weeks. The animals were divided into three experimental groups: ApoEC (n=6-8), which did not receive intervention; ApoEP (n=6-8), with periodontitis caused; and ApoEP+PBM (n=6-8), with periodontitis caused and NIR-PBM with an energy density of 6 J per session (808 nm, continuous wave, ø ~ 3 mm2, 100 mW) for 60 seconds for seven consecutive days. Alveolar bone loss was assessed using scanning electron microscopy of the mandible; the quantification of reactive oxygen species (ROS) by flow cytometry; plasma cholesterol levels; inflammatory activity (myeloperoxidase levels) and plasma oxidative stress (lipid peroxidation) through biochemical assays. The success of PD caused by ligation was due to the significant alveolar bone loss in the ApoEP group compared with the ApoEC group. Periodontitis increased plasma cholesterol levels in the ApoEP group, compared to ApoEC. Animals in the ApoEP group showed higher levels of myeloperoxidase (MPO) when compared to the ApoEC group. The levels of superoxide anion and hydrogen peroxide are different when compared to ApoEP and ApoEC, showing an increase in ROS production in animals with periodontitis. The ApoEP+PBM group presented reduced levels when compared to animals in the ApoEP group, and the ApoEC group presented lower levels of hydrogen peroxide than the ApoEP+PBM group. Higher levels of lipid peroxidation were presented in the ApoEP group than in the ApoEC group and animals that received NIR-PBM had lower levels than untreated animals, implying that periodontitis increases oxidative stress, but PBM treatment decreases it. these levels. We conclude that in hypercholesterolemic animals, NIR-PBM is effective in reducing systemic disruptions involved in the progression and worsening of periodontal disease. Furthermore, further studies with longer evaluation times are needed to elucidate the effects of PBM on experimental periodontitis related to hypercholesterolemia
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    Efeitos da terapia a laser de baixa intensidade sobre as células da gengiva de camundongos com periodontite experimental
    (Universidade Federal do Espírito Santo, 2024-07-15) Braga, Larissa Trarbach Figueiredo; Porto, Marcella Leite; Meyrelles, Silvana dos Santos; https://orcid.org/0000-0003-0167-4093; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; Campagnaro, Bianca Prandi; https://orcid.org/; http://lattes.cnpq.br/; Rosetti, Elizabeth Pimentel; https://orcid.org/; http://lattes.cnpq.br/
    Introduction: Periodontitis is a multifactorial chronic inflammatory disease associated with dysbiotic microorganisms that, through the presence of bacterial biofilm, alter the homeostatic environment of the periodontium and impair systemic health. The inflammatory changes hinder the host's immune response and cause degradation of the protective and supportive tissues of the teeth, which can consequently lead to tooth loss. Low-Level Laser Therapy (LLLT) has been studied for its potential to reduce inflammation and influence cellular processes with positive results. However, its use in conjunction with periodontal treatments remains debatable. Objectives: To evaluate the effect of LLLT on reactive oxygen species (ROS) and apoptosis in gingival tissue, and on systemic parameters related to inflammation and oxidative stress caused by experimental periodontitis. Methods: This experimental research was conducted on 16-week-old C57/BL6 mice, randomly divided into four groups: control (C) - no intervention; control+LLLT (C+L) - no intervention and treated with LLLT; periodontitis (P) - with ligature-induced periodontitis; periodontitis+LLLT (P+L) - with ligature-induced periodontitis and treated with LLLT. After 28 days of induction, the ligature was removed and the animals were treated with scaling and root planing (SRP), and LLLT was applied for 3 days (4 joules per session). At the end of the treatment, the animals were euthanized; the gingiva, mandible, and plasma were separated for analysis. In the mandible, to confirm periodontitis, we measured alveolar bone loss using scanning electron microscopy (SEM). In gingival tissue, ROS levels and cell apoptosis were evaluated by flow cytometry. In plasma, inflammatory activity was analyzed through the enzyme myeloperoxidase (MPO), and protein oxidation was assessed using the advanced oxidation protein products (AOPP) assay, both by spectrophotometry; cytokine levels were measured using flow cytometry. Results: Periodontitis was confirmed. LLLT reduced superoxide anion and hydrogen peroxide levels in gingival tissue by approximately 18% and 26%, respectively. Apoptosis in gingival cells was 32% lower in LLLT-treated animals with periodontitis. Systemic inflammatory activity and protein oxidation decreased by 49.3% and 38.6%, respectively. In the P+L group, plasma levels of pro-inflammatory cytokines IL-6 and IL-12p70 were reduced, while the anti-inflammatory cytokine IL-10 increased compared to the P group (p<0.05). Conclusion: In this experimental model, the use of LLLT effectively regulated ROS levels and apoptosis in gingival tissue, influencing plasma markers of oxidative stress and inflammation.